We have assessed the utility of autologous mesenchymal stem cell (MSC) peripheral vein infusion as a possible therapeutic modality for patients with end-stage liver diseases.Forty post-hepatitis C virus (HCV) disease were randomized into two groups: Group 1 (GI): 20 who received granulocyte colony-stimulating factor (G-CSF) 5 days followed by MSCs peripheral-vein and group 2 (GII): regular liver-supportive treatment only (control group).In MSC-infused (GI), 54% showed near normalization...

Abstract Allogeneic hematopoietic stem cell transplant (HSCT) for adults with severe sickle disease (SCD) is potentially curative but not commonly utilized therapy due to complications such as graft failure (GF) and organ toxicity. Herein, we are reporting our long‐term outcome data of non‐myeloablative (NMA) HSCT in SCD emphasis on factors predicting event free survival (EFS). Adults undergoing NMA match‐related donor allogeneic from 2015 2021 at least 12 months follow‐up were included. A...

Abstract Improved sperm motility is necessary for successful passage through the female genital system, efficacious fertilization, and a greater probability of pregnancy. By stimulating mitochondrial respiratory chain, low-level laser photobiomodulation has been shown to increase velocity. The chain in mitochondria primary site action cytochrome c oxidase because it can absorb light visible infrared ranges. present study aimed investigate effects red 650 nm, near (NIR) 980 combination both...

Abstract To investigate the potential of nitric oxide (NO) and urinary nerve growth factor (NGF) as indicators therapeutic outcomes in overactive bladder (OAB) patients undergoing low-level laser therapy (LLLT) via a prospective randomized controlled trial. Fifty OAB participated study were subjected to LLLT using 650-nm irradiation. The employed trial design. Nitric urine NGF levels assessed before after intervention evaluate their correlation with outcomes. provided evidence supporting...

It is critical to characterize the degradation products of therapeutic drugs determine their safety as these may possess fatal effects on human physiological system. Favipiravir (FVP), a novel anti-Covid-19 drug, that recently used all over world with great impact humanity was our target explore more about its toxicity, margins safety, and degradants in different conditions. The goal this study identify, characterize, confirm structures FVP oxidative alkaline breakdown products, well assess utilizing

MicroRNAs regulate the expression of many genes and subsequently control various cellular processes, such as immune response to viral infections mediated by type I interferon (IFN). In this study, pattern two interferon‐related microRNAs, miR‐146a miR‐155, was examined in healthy HCV‐genotype‐4‐infected peripheral blood mononuclear cells (PBMCs) using qRT‐PCR. contrast other infections, similar both infected PBMCs. This could be attributed attenuation IFN pathway HCV, which assessed...

A new green-validated and highly sensitive electrochemical method for the determination of molnupiravir (MOV) has been developed using cyclic voltammetry. The proposed analytical platform involves use a disposable laboratory-made screen-printed reduced graphene oxide 2.5% modified electrode (rGO-SPCE 2.5%) first time to measure MOV with high specificity. surface morphology sensor was investigated by scanning electron microscope armed an energy-dispersive X-ray probe. fabricated attained...

Abstract Background Hepatitis C virus ( HCV ) has a lower prevalence in children and knowledge is limited regarding the natural outcome of infection children. Aim To study risk factors acquisition predictors persistence Egyptian Methods Children, 1–9 years age, were evaluated for (anti‐ positive regardless viraemia) ‐ RNA positive) at two paediatric hepatology clinics airo enrolment 3 monthly intervals. Spontaneous clearance was defined as ≥ anti‐ antibody tests with negative least 6 months...

The Emerging Role of Circular RNA Homeodomain Interacting Protein Kinase 3 and 0046367 through Wnt/Beta-Catenin Pathway on the Pathogenesis Nonalcoholic Steatohepatitis in Egyptian Patients

Abstract Benign prostatic hyperplasia (BPH) is a chronic, progressive disease characterized by mesenchymal cell-predominance and stromal glandular cell-hyperproliferation. Although, the precise cause of BPH unknown, it believed to be associated with hormonal changes in aging men. Despite androgens ageing are likely play role development BPH, pathophysiology remains uncertain. This paper aims evaluate diagnostic efficacy platelet-to-lymphocyte ratio (PLR), neutrophil–lymphocyte (NLR) systemic...

Hepatits C virus (HCV) genotype 4 (GT4) shows low treatment response rates and discrepancies when compared to other genotypes. However, the reason underlying these remains unclear due limited number of studies on GT4. microRNA‑155 (miR‑155) is a noteworthy example discrepancy in GT4, as it was found be upregulated genotypes 1, 2 3 HCV infection, but downregulated GT4‑HCV‑infected peripheral blood mononuclear cells (PBMCs). The present study aimed investigate expression miR‑155 PBMCs, serum...

This study aimed to present the outcomes of retrograde and antegrade ureteroscopic laser lithotripsy in treatment proximal ureteral stones ranging size from 10 20 millimeters diameter.

This prospective cohort study aimed to assess the influence of stem cell therapy (SCT) on health-related quality life (HRQOL) by using SF-36 v2 and elucidate objective clinical variables subjective HRQOL. The included 100 chronic liver disease patients (50 received SCT, 50 supportive medical treatment (SMT)). Both groups completed a modified form before at 1-, 3-, 6-, 12-month intervals. Fifty healthy Egyptian volunteers were enrolled in once. SCT SMT showed significantly lower pretherapy SF...

AIM:To investigate the association of functional monocyte chemotactic protein-1 (MCP-1 ) promoter polymorphism (A-2518G) with spontaneous bacterial peritonitis (SBP). METHODS:Fifty patients post-hepatitis C liver cirrhosis and ascites were categorized into two groups; group Ⅰ included 25 SBP Ⅱ free from SBP.In addition, a 20 healthy volunteers included.We assessed 11793 September 7, 2014|Volume 20|Issue 33| WJG|www.wjgnet.com

microRNA (miRNA) expression in organs does not always represent their quantity serum. A disparity the of miR‑181a has been reported tissues and serum hepatocellular carcinoma (HCC) patients. Since hepatitis C virus (HCV) is a major cause HCC never studied HCV, present study aimed to investigate profile genotype 4 (GT4)‑HCV patients evaluate whether this pattern also apparent HCV. RNA was extracted from liver tissues, peripheral mononuclear cells (PBMCs) samples GT4‑HCV‑infected healthy...

Green, economic and sensitive two spectrofluorometric methods were developed for the quantitation of flibanserin (FB) in different matrices, which are based on FB native fluorescence properties. The first technique depends measuring relative intensity directly at emission excitation wavelengths(λem/λex) (371 nm/247 nm), while second is a derivative (D1) method, peak amplitudes 351 nm. Linear regressions observed range 0.1–1.5 μg/mL both methods. Moreover, efficiently extended to analyze...

HSV-1 encephalitis (HSE) is the most common cause of fatal sporadic in United States. HSE adults commonly due to reactivation HSV central nervous system (CNS) which results CNS necrosis leading neurological compromise. The symptoms include altered mentation, fever, seizures, and focal deficits. involves damage temporal lobes however can rarely involve other structures such as brainstem cerebellum. Immunocompromised status may increase risk atypical HSE. involvement brainstem, particularly...

Egypt has one of the highest prevalences hepatitis C virus (HCV) infection worldwide. Although IL28B gene polymorphism been shown to modify course chronic HCV infection, this not properly assessed in Egyptian population.The rs12979860 single nucleotide (SNP) was therefore examined 256 HCV-infected patients (group II) at different stages disease progression and 48 healthy volunteers I). Group II subdivided into GII-A (chronic patients, n=119), GII-B (post cirrhosis, n=66) GII-C (HCC on top...

A new green-validated and highly sensitive electrochemical method for the determination of molnupiavir (MOV) has been developed using cyclic voltammetry. The proposed analytical platform involves use disposable screen-printed reduced graphene oxide 2.5% electrode (SPrGOE 2.5%) first time to measure MOV with high specificity. surface morphology sensor was investigated by a scanning electron microscope armed an energy-dispersive X-ray probe. fabricated attains improved sensitivity when sodium...

This study describes a rapid and sensitive HPLC method using fluorescence detection, which enables the determination of Sitagliptin mono phosphate (SIT) Itopride Hydrochloride (ITP) as internal standard with good accuracy precision to allow its detection in human plasma. SIT ITP were extracted by liquid extraction (LLE) ethyl acetate. All factors studied optimize chromatographic conditions full factorial design central composite for screening optimization respectively. Chromatographic...

Purpose We studied the sonographic appearances of hepatic hydatid cysts and used sonography to follow up these after sonographically guided aspiration using PAIR technique. Methods Included in this study were 362 patients with 558 cysts. Initial examination revealed 510 noncomposite 48 composite (18 rosette 30 honeycomb pattern). Noncomposite (510 cysts) aspirated treated scolicidal agents Sonographic follow-up was done for 5 years aspiration. Results There immediate detachment germinal...

Summary. Hepatitis C virus (HCV) is a major health concern in Egypt being highly prevalent among Egyptians. The two genders experience different responses to HCV infection and show variations response interferon (IFN)-based therapy that may be attributed sex hormones. We previously demonstrated the suppressive effect of 17β-estradiol (E2) on expression IFN-stimulated gene MxA HCV-infected peripheral blood mononuclear cells (PBMCs). selective oestrogen receptor (ER) modulator Tamoxifen has...