A5083963101- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Hepatitis C virus research
- Circular RNAs in diseases
- Lipid metabolism and biosynthesis
- RNA modifications and cancer
- Immune Cell Function and Interaction
- interferon and immune responses
- Extracellular vesicles in disease
- RNA Research and Splicing
- RNA regulation and disease
- HIV Research and Treatment
- Reproductive System and Pregnancy
- Colorectal Cancer Treatments and Studies
- Cancer, Lipids, and Metabolism
- Mesenchymal stem cell research
- Endoplasmic Reticulum Stress and Disease
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Biochemical and Molecular Research
- COVID-19 Clinical Research Studies
- Pharmacological Receptor Mechanisms and Effects
- HIV/AIDS drug development and treatment
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
German University in Cairo
2012-2018
IBM (Egypt)
2016
Despite the vast vaccination campaigns against SARS-CoV-2, vaccine-resistant variants have emerged, and COVID-19 is continuing to spread with fear of emergence new that are resistant currently available anti-viral drugs. Hence, there an urgent need discover potential host-directed - rather than virus-directed therapies COVID-19. SARS-CoV-2 enters host cells through binding viral spike (S)-protein angiotensin-converting enzyme 2 (ACE2) receptor, rendering port entry attractive therapeutic...
Meis1 is a transcription factor involved in numerous functions including development and proliferation has been previously shown to harness cell cycle progression. In this study, we used silico analysis predict that miR-499-5p targets Malat1 sponges miR-499-5p. For the first time, demonstrated overexpression of led downregulation mRNA protein C166 cells by directly binding its 3'UTR. Moreover, knocking down increased expression, subsequently suppressing Meis1. Through BrdU incorporation...
Introduction The role of miRNAs in regulating variable molecular functions has been sought by scientists for its promising utility the immune response and, hence, treating various diseases. In hepatocellular carcinoma (HCC) specifically, a reduction number and efficiency circulating intrahepatic natural killer (NK) cells reported. Our project aims to investigate miR-216a-3p regulation NK cell cytotoxicity, especially since it plays tumor suppressor context HCC. Methods To achieve our aim, we...
MicroRNAs regulate the expression of many genes and subsequently control various cellular processes, such as immune response to viral infections mediated by type I interferon (IFN). In this study, pattern two interferon‐related microRNAs, miR‐146a miR‐155, was examined in healthy HCV‐genotype‐4‐infected peripheral blood mononuclear cells (PBMCs) using qRT‐PCR. contrast other infections, similar both infected PBMCs. This could be attributed attenuation IFN pathway HCV, which assessed...
miR-let-7a is a tumor suppressor miRNA with reduced expression in most cancers. Methylation of MIRLET7A3 gene was reported to be the cause this suppression several cancers; however, it not explicitly investigated hepatocellular carcinoma (HCC). We aimed at investigating and molecular mode HCC, identifying drug-targetable networks, which might affected by its abundance. Our results illustrated significant repression miR-let-7a, correlated hypermethylation origin MIRLRT7A3. This further...
Hepatits C virus (HCV) genotype 4 (GT4) shows low treatment response rates and discrepancies when compared to other genotypes. However, the reason underlying these remains unclear due limited number of studies on GT4. microRNA‑155 (miR‑155) is a noteworthy example discrepancy in GT4, as it was found be upregulated genotypes 1, 2 3 HCV infection, but downregulated GT4‑HCV‑infected peripheral blood mononuclear cells (PBMCs). The present study aimed investigate expression miR‑155 PBMCs, serum...
HCV entry involves a complex interplay between viral and host molecules. During post-binding interactions, the E2 complexes with CD81 receptor for delivery to tight junction proteins CLDN1 OCLN, which aid in internalization. Targeting receptors represents an appealing approach inhibit infectivity. This study aimed at investigating impact of targeting by microRNAs on miR-155 was previously shown target 3'UTR mRNA. Therefore, used as control this study. In-silico analysis luciferase reporter...
microRNA (miRNA) expression in organs does not always represent their quantity serum. A disparity the of miR‑181a has been reported tissues and serum hepatocellular carcinoma (HCC) patients. Since hepatitis C virus (HCV) is a major cause HCC never studied HCV, present study aimed to investigate profile genotype 4 (GT4)‑HCV patients evaluate whether this pattern also apparent HCV. RNA was extracted from liver tissues, peripheral mononuclear cells (PBMCs) samples GT4‑HCV‑infected healthy...
BACKGROUND Because there is a global shortage of intravenous immunoglobulin, need for new products to fill the gap. STUDY DESIGN AND METHODS This was multicenter, open‐label study investigating safety and efficacy newly developed mini‐pool immunoglobulin G children with immune thrombocytopenia. Seventy‐two patients ages 1 18 years diagnosed (<1 month) thrombocytopenia who had platelet counts from 5 20 × 10 9 /L no serious bleeding were recruited four centers in Egypt. Eligible randomized...
Background: Identification of factors to detect and improve chemotherapy-response in cancer is the main concern. microRNA-372-3p (miR-372-3p) has been demonstrated play a crucial role cellular proliferation, apoptosis metastasis various cancers including Hepatocellular Carcinoma (HCC). However, its contribution towards Doxorubicin (Dox) chemosensitivity HCC never studied. Objective: This study aims investigate potential miR-372-3p enhancing Dox effects on cell line (HepG2). Their correlation...
Hepatitis C Virus (HCV) promotes lipid droplet (LD) formation and perturbs the expression of LD associated PAT proteins ADRP TIP47, to promote its own lifecycle. HCV enhances TIP47 suppresses by displacing it from surface in infected cell models. We have previously shown that suppression miR‐148a miR‐30a decreased intracellular LDs RNA. Thus, this study aimed at examining whether microRNA‐mediated would limit HCV‐dependent displacement LDs. was examined 21 HCV‐infected liver biopsies 9...
Aims: To examine the regulation of SREBP-1c and CAV1 by microRNA-29a (miR-29a) in cells infected with hepatitis C virus (HCV) an attempt to control HCV-induced non-alcoholic fatty liver disease. Methods: In order manipulation miR-29a, oleic acid (OA)-treated JFH-I-infected Huh-7 were used. OA was added 24 h post-transfection gene expression investigated qRT-PCR at 48 post treatment. The functional impact observed alteration analyzed examining lipid droplet (LD) triglyceride (TG) content 72...
Background: Doxorubicin (DOX) is the most common drugs used in cancer therapy, including Hepatocellular Carcinoma (HCC). Drug resistance, one of chemotherapy’s significant problems. Emerging studies have shown that microRNAs (miRNAs) could participate regulating this mechanism. Nevertheless, impact miRNAs on HCC chemoresistance still enigmatic. Objective: Investigating role miR-520c-3p enhancement anti-tumor effect DOX against HepG2 cells. Methods: Expression profile for liver related (384...
Bioinformatics analysis revealed that miR‐148a and miR‐30a potentially target TIP47. Expression profiling showed both microRNAs were downregulated, while TIP47 was upregulated in liver biopsies of HCV‐infected patients. Forcing the expression JFH‐I infected, oleic acid‐treated Huh7 cells, significantly suppressed reduced cellular LDs with marked decrease viral RNA. This study shows miR‐30a, regulate HCV infected cells.