Abstract Discovery of novel biomarkers is critical for early diagnosis acute coronary syndrome (ACS). Serum metabolite profiling ST-elevation myocardial infarction (STEMI), unstable angina (UA) and healthy controls was performed using gas chromatography mass spectrometry (GC/MS), solid-phase microextraction coupled to (SPME-GC/MS) nuclear magnetic resonance ( 1 H-NMR). Multivariate data analysis revealed a metabolic signature that could robustly discriminate STEMI patients from both UA...

Chemometrics, which applies mathematical and statistical methods to aid in experimental design, data analysis, process optimization, is gaining more importance. It plays an important role enhancing the sustainability of analytical when integrated with green chemistry. Applying chemometrics liquid chromatographic can speed up method, saving time, energy, solvent consumption. This would be reflected by a low energy footprint environmental impact. review discusses application chemometric like...

Stanozolol, a synthetic derivative of testosterone, is one the common doping drugs among athletes and bodybuilders. It metabolized to large extent metabolites are detected in urine for longer duration than parent compound. In this study, novel dummy molecularly imprinted polymer (DMIP) developed as sorbent solid-phase extraction stanozolol from spiked human samples. The optimized DMIP composed template, methacrylic acid functional monomer, ethylene glycol dimethacrylate cross-linker ratio...

Abstract Wheat is considered as the most important cereal grain globally. It has a vast economic importance it used in producing bread, pastries, and household flour serving food for livestock among other uses. Different biological activities of wheat were correlated with presence polyphenols due to their antioxidant preventative capabilities. can also be an antidiabetic, anti-inflammatory, anticancer, antimicrobial, antiaging agent. Omics established itself during past 20 years crucial tool...

Moringa oleifera (MO) known as the miracle tree is a famous nutritional source in many countries. In this study, neuroprotective activity of MO seeds was investigated. Fractions 70% ethanol seed extract were injected at dose 250 mg kg-1 day-1 to albino rats for 15 days, after-which induction dementia done using 100 mg/kg AlCl3 over 30 days. Results revealed that all fractions ameliorated effects where methylene chloride and ethyl acetate fractions, containing major bioactive compound...

The present study describes a generic strategy using capillary electrophoretic (CE) method for chiral enantioseparation of anti-Alzheimer drugs, namely, donepezil (DON), rivastigmine (RIV), and antifungal ketoconazole (KET), Itraconazole (ITR), fluconazole (FLU), sertaconazole (SRT) in which these drugs have different basic acidic properties. Several modified cyclodextrins (CDs) were applied racemates such as highly sulfated α, γ CDs, hydroxyl propyl-β-CD, Sulfobutyl ether-β-CD. starting...

The nanoparticle (NP) protein corona represents an interface between biological components and NPs, dictating their cellular interaction fate. To assess the success of targeting, NPs modified with targeting ligands are incubated target cells in serum-free culture medium or presence fetal bovine serum (FBS). In former, role is overlooked, latter, effects a that does not represent one forming humans nor respective disease state considered. Via proteomic analysis, we demonstrate how difference...

Accumulating evidence has stipulated a strong correlation between vitamin D (vitD) deficiency and cardiovascular disease (CVD); however, mechanistic link is missing. This study investigated the association of vitD with endothelial dysfunction parameters. Subjects comprised male patients verified coronary artery (CAD) (n=69) age- sex-matched controls (n=20). 25-Hydroxyvitamin [25(OH)D] was determined using high performance liquid chromatography ultraviolet detection whereas asymmetric...

Although high-performance liquid chromatography–mass spectrometry (HPLC–MS) is adopted as the method of choice for determination vitamin D and its metabolites in plasma, yet unavailability this expensive detection technique many clinical laboratories makes ultraviolet (UV) alternative places worldwide. In regard, parameters affecting HPLC separation vitamins D2, D3 their hydroxyl plasma a systematic way would put an end to irrelevant trials more optimization. A new robust HPLC–UV was...

The chemical compositions of essential oils from cumin and coriander fruits were investigated using GLC GLC/MS. Forty six compounds identified in both oils. major constituents oil as γ- terpinene (22.7%), β-pinene (19.2%), cuminaldehyde (18.0%), p-cymene (11.5%), whereas linalool (70.43%) is the most abundant component oil. antioxidant anti-inflammatory activity cytotoxicity assessed. Cumin exhibited stronger (IC50 values, 2.37 mg/ml for DPPH• assay 27.7 μg/ml OH• assay) compared to Both...

Analysis of water-soluble vitamins has been tremendously approached through the last decades. A multitude HPLC methods have reported with a variety advantages/shortcomings, yet, design space analysis these was not defined in any reports. As per food and drug administration (FDA), implementing quality by approach for commercially available mixtures is hypothesized to enhance pharmaceutical industry via facilitating process analytical method development approval. This work illustrates...

Nitrated fatty acids (NO2-FAs) are a newly discovered class of biologically active compounds with distinct biochemical features that induce physiologically beneficial alterations in transcriptional regulatory protein function, leading to variety modulatory and protective actions. The most common NO2-FAs identified vivo so far nitro oleic acid (NO2-OA), linoleic (NO2-LA) its structural isomer nitro-conjugated (NO2-cLA). Analytical limitations compromise accurate quantitation these endogenous...

ABSTRACT The present work aimed to investigate the predictability of chromatographic behavior for separation underivatized amino acids on ristocetin A, known as Chirobiotic R, using a DryLab high‐performance liquid chromatography (HPLC) method development software, which is typically used predict effect changing various parameters resolution in reversed phase mode. After implementing basic runs, and judging via computed map, it can be deduced that chiral recognition mechanisms tend towards...