Abstract Triple‐negative breast cancer (TNBC) represents an aggressive subtype. Among young females, TNBC is the leading cause of cancer‐related mortalities. Recently, long noncoding RNAs (lncRNAs) are representing a promising pool regulators for tuning aggressiveness several solid malignancies. However, this still needs further investigations in TNBC. The main aim study to unravel expression pattern sONE lncRNA and its mechanistic role Results showed that restrictedly expressed patients;...

Solidago virgaurea L. (Asteraceae) is traditionally used as an anti-inflammatory for the treatment of various symptoms including cystitis. However, little known concerning constituents responsible this activity and mechanism their action.To assess phenolic-rich fraction S. aerial parts in rats, isolate major compounds present.An HPLC method was developed analysis (EtFr). The vivo EtFr four isolated (at 25 50 mg/kg) were assessed adult male rats using carrageenan-induced rat paw oedema model....

This study focused on studying the impact of flavonoids isolated from Cleome droserifolia HCC cell lines and to further unveil their possible TP53 its downstream tumor suppressor miRNAs. Three flavonol glycosides were C. namely, Isorhamnetin-3-O-β-D-glucoside (1), Quercetin-3`-methoxy-3-O-(4``-acetylrhamnoside)-7-O-α-rhamnoside (2), Kaempferol-4`-methoxy-3,7-O-dirhamnoside (3). They showed a concentration time dependent reduction in cellular viability anchorage-independent growth cells....

Sulphur compounds in sulphur rich food have been shown to significantly reduce the risk of cancer development. One such compound is sulforaphane (SF), a chemopreventive agent identified broccoli (F. cruciferae). In this study, SF content was assessed extracts several crucifer vegetables including broccoli, brussels sprout, green cabbage, red Chinese kale and turnip, parallel with anticancer antioxidant activity. Among tested crucifers, cabbage demonstrated pronounced effect against A-549...

A comparative study was carried out with ethanolic (80%) extracts from leaves and cell cultures of three Callistemon species, namely C. lanceolatus (CL), viridiflorous (CV), comboynensis (CC). Cell suspensions the species were grown in liquid Murashige Skoog (MS) medium (100 ml) supplemented 0.9 mg·g-1 kinetin combination 1.1 NAA. The CL leaf extract standardized to contain highest amount phenolics (104 ± 2.0 mg·g-1), followed by CC (95.8 1.2 mg·g-1) CV (79.8 4.6 mg·g-1). On other hand,...

The activating immune ligands, MICA/B, act as a "kill me" signal through the NKG2D receptor expressed on natural killer (NK) cells. Recently, oncogenic miR-20a was found to mediate escape repressing MICA/B levels in breast cancer (BC) However, targeting miR-20a-MICA/B using compounds has rarely been investigated. Our group successfully isolated 3'-O-acetylvitexin that showed cytotoxic effects against colon cells but never evaluated BC. aim is investigate of BC cell lines and further...

Recently, a novel crosstalk between non-coding RNAs (ncRNAs) has been casted. However, this seldom investigated in metastatic BC (mBC). H19 and miR-486-5p role mBC are controversial. ICAM-1 is recently recognized engine mBC. Natural compounds were found to alter ncRNAs/target circuits. Yet, Hesperitin's modulatory altering such circuits never mBC.The aim of study investigate the impact hesperitin on miR-486-5p/H19/ICAM-1 axis.BC patients (n=20) recruited study. Bioinformatic analysis was...

Diabetes mellitus is a chronic disease affecting the globe and its incidence increasing pandemically. The use of plant-derived natural products for diabetes management great interest. Polar fraction Artemisia annua L. leaves has shown antidiabetic activity in vivo. In present study, three major compounds were isolated from this polar fraction; namely, 3,5-dicaffeoylquinic acid (1); 4,5-dicaffeoylquinic (2), 3,4- dicaffeoylquinic methyl ester (3), using VLC-RP-18 HPLC techniques. potential...

Alzheimer's disease (AD) is an irreversible neurodegenerative disorder manifested by cognitive deterioration where the available treatments failed to delay its progression. The objective of this study was investigate neuroprotective activity in aluminum chloride (AlCl3)-induced AD vivo model and phytochemical profile traditional Egyptian herb Mentha longifolia (Ml). Male albino rats were injected with Ml fractions essential oil for 15 days followed AlCl3 30 days. Oxidative stress...

The active compounds present in Hypericum perforatum L. (Hypericaceae) include hyperforin, hypericins and flavonoids, which are assumed to be responsible for the activity of extract treatment wounds scars. study aimed incorporate H. standardized a known content phloroglucinols, naphthodianthrones polyphenolic into an effective transdermal drug delivery system capable entrapping both lipophilic hydrophilic constituents form niosomal gels wound treatment. An 80% ethanol (HE) was prepared on...

The hepatoprotective activities of two traditionally used plants, Cleome droserifolia (Forssk.) Delile, Cleomaceae, and Artemisia annua L., Asteraceae, were recently reported. However, the biologically active metabolites responsible for this activity not identified. aqueous extract C. aerial parts, polar fraction A. leaves screened their antioxidant using 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) assay. in vitro viability HepG-2 cells treated with CCl4 extracts assessed by MTT...

Diabetes is a major health problem that associated with high risk of various complications. Medicinal plants hold great promise against diabetes. The traditional use

Moringa oleifera (MO) known as the miracle tree is a famous nutritional source in many countries. In this study, neuroprotective activity of MO seeds was investigated. Fractions 70% ethanol seed extract were injected at dose 250 mg kg-1 day-1 to albino rats for 15 days, after-which induction dementia done using 100 mg/kg AlCl3 over 30 days. Results revealed that all fractions ameliorated effects where methylene chloride and ethyl acetate fractions, containing major bioactive compound...

Adenylyl cyclase Rv2212 from Mycobacterium tuberculosis has a domain composition identical to the pH‐sensing isoform Rv1264, an N‐terminal regulatory and C‐terminal catalytic domain. The maximal velocity of was highest all 10 mycobacterial cyclases investigated date (3.9 µmol cAMP·mg −1 ·min ), whereas ATP substrate affinity low (SC 50 = 2.1 m ATP). Guanylyl side activity absent. activities kinetics holoenzyme alone were similar, i.e. in distinct contrast Rv1264 adenylyl cyclase, which is...

Different parts of the fruit Punica granatum L. family Punicaceae cultivated in Egypt were extracted and standardized to be assessed for their anticancer antioxidant properties.An HPLC method was modified validated standardization using ellagic acid (EA) as a marker.The 50% ethanol proven better solvent extraction than 70% ethanol, hydroalcoholic extract contain 5.9 ± 0.15 % total polyphenols compared 4.4 0.35 extract.The extracts whole fruit, pulp peels 0.3 0.05, 0.02 0.01 1.9 0.1 EA,...

The antidiabetic activities of different extracts and fractions Balanites aegyptiaca were tested in cultured C2C12 skeletal muscle cells 3T3-L1 adipocytes. An 18-h treatment with 200 µg/mL the sugars fraction (A1) showed highest activity as it increased basal glucose uptake by 52% cells; which is twice 100 nM insulin [insulin equivalent (IE) = 2.0 ± 0.07]. dichloromethane (E) ethyl acetate (F) successive exerted 37 41% increase uptake, respectively. Only E F accelerated triglyceride...

The aqueous and alcoholic extracts of the fresh aerial parts Lippia citriodora Kunth, family Verbenaceae, cultivated in Egypt, exhibited variable antiinflammatory, antipyretic, analgesic antioxidant properties. Three phenolic compounds; two acids, dihydrocaffeic acid (1) 4-hydroxycinnamic (3) a flavonoid glycoside, luteolin-7-O-glucoside (2), were isolated identified from ethyl acetate fraction plant. structures compounds elucidated on basis spectral analysis. effects explant type, method...