A5047078549- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Hepatitis B Virus Studies
- Genetic factors in colorectal cancer
- Cancer-related molecular mechanisms research
- Cancer-related Molecular Pathways
- SARS-CoV-2 and COVID-19 Research
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Lymphoma Diagnosis and Treatment
- Colorectal Cancer Treatments and Studies
- Cancer Cells and Metastasis
- Bladder and Urothelial Cancer Treatments
- RNA modifications and cancer
- Viral-associated cancers and disorders
- BRCA gene mutations in cancer
- Circular RNAs in diseases
- Liver physiology and pathology
- Monoclonal and Polyclonal Antibodies Research
- Pancreatic and Hepatic Oncology Research
- Cancer Research and Treatments
- COVID-19 Clinical Research Studies
- Urinary and Genital Oncology Studies
- Cervical Cancer and HPV Research
Cairo University
2016-2025
Gilead Sciences (United States)
2022
Children Cancer Hospital
2011-2022
National Cancer Institute
2022
Cancer Institute (WIA)
2002-2021
Institute of Zoology
2018
Misr University for Science and Technology
2018
Nile University
2016
Deleted Institution
2015
National Cancer Institute
2013
SARS-CoV-2 across Africa The impact of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has been hard to track in African countries, largely because patchy data. Wilkinson et al . curated viral genomes collected 2021 from several countries continent. Outbreaks during 2020 each country were initiated by imported cases, mostly Europe. As developed, case numbers likely many times higher than reported, and subsequent waves appear have more severe. Consequently,...
Investment in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing Africa over the past year has led to a major increase number of sequences that have been generated and used track pandemic on continent, now exceeds 100,000 genomes. Our results show an African countries are able sequence domestically highlight local enables faster turnaround times more-regular routine surveillance. Despite limitations low testing proportions, findings from this genomic surveillance study...
We have assessed the utility of autologous mesenchymal stem cell (MSC) peripheral vein infusion as a possible therapeutic modality for patients with end-stage liver diseases.Forty post-hepatitis C virus (HCV) disease were randomized into two groups: Group 1 (GI): 20 who received granulocyte colony-stimulating factor (G-CSF) 5 days followed by MSCs peripheral-vein and group 2 (GII): regular liver-supportive treatment only (control group).In MSC-infused (GI), 54% showed near normalization...
The identification of new high-sensitivity and high-specificity markers for hepatocellular carcinoma (HCC) is essential. We aimed at identifying serum microRNAs (miRNAs) as potential biomarkers early detection HCC on top hepatitis C virus (HCV) infection. investigated expression 13 miRNAs in 384 patients with HCV-related chronic liver disease (192 HCC, 96 cirrhosis (LC), (CHC)) addition to healthy participants enrolled a control group. miRNA was performed using real-time quantitative...
Colorectal cancer is a common all over the world. Aberrations in cell cycle checkpoints have been shown to be of prognostic significance colorectal cancer.The expression cyclin D1, A, histone H3 and Ki-67 was examined 60 cases for co-regulation impact on overall survival using immunohistochemistry, southern blot situ hybridization techniques. Immunoreactivity evaluated semi quantitatively by determining staining index studied proteins.There significant correlation between D1 gene...
The only presently viable treatment for end-stage liver disease is whole organ transplantation. However, there are insufficient livers available. aim of the present study to provide autologous bone marrow-derived stem cells as a potential therapeutic patients with cirrhosis. This retrospective chart review cell in 48 patients, 36 chronic hepatitis C-induced and 12 autoimmune disease. For all granulocyte colony-stimulating factor was administered mobilize their hematopoietic cells. Following...
Aim and background: MicroRNAs (miRNAs) are a class of naturally occurring small noncoding RNAs that regulate gene expression, cell growth, differentiation apoptosis by targeting mRNAs for translational repression or cleavage. The present study was conducted to miRNAs in Egyptian breast cancer (BC) their relation metastasis, tumor invasion addition association with the ER PR statuses. Methods: Real Time RT-PCR performed identify miRNA expression level eight metastatic-related genes 40 samples...
Aim The study was designed to assess the possibility of using circulating miRNAs (serum miRNAs) as diagnostic biomarkers in colorectal cancer (CRC) and identify their candidates for targeted therapy. Methods involved two sample sets: 1- a training set which included 90 patients with related disease (30 CRC, 18 inflammatory bowel (IBD), colonic polyps (CP) 24 different symptoms but without any colonoscopic abnormality who were enrolled control group) 2- validation 100 CRC patients. Serum...
AIM:To assess the utility of an autologous CD34 + and CD133+ stem cells infusion as a possible therapeutic modality in patients with end-stage liver diseases. METHODS:One hundred forty endstage diseases were randomized into two groups.Group 1, comprising 90 patients, received granulocyte colony stimulating factor for five days followed by CD34+ CD133 cell portal vein.Group 2, 50 regular treatment only served control group. RESULTS:Near normalization enzymes improvement synthetic function...
In the current study, prognostic and predictive values of serum transforming growth factor-β1 (TGF-β1), insulin-like factor I (IGF-I)/IGF-I receptor (IGF-IR) vascular endothelial factor-A (VEGF-A) were evaluated in triple-negative non-triple-negative breast cancer (TNBC non-TNBC). The aim was to identify a group serological biomarkers possible candidates for targeted therapy patients with TNBC non-TNBC. Protein levels TGF-β1, IGF-I/IGF-IR VEGF-A measured 43 TNBC, 53 non‑TNBC 20 normal...
The role of Epstein-Barr virus (EBV) in breast carcinogenesis is still controversial. Unraveling this relationship potentially important for better understanding cancer etiology, early detection and possibly prevention cancer. aim the current study to unravel association between EBV primary invasive (PIBC) two different Arab populations (Egyptian Iraqi women).The was done on paraffin-embedded tissues 40 Egyptian 50 patients with PIBC addition 20 normal as controls each group. Both neoplastic...
Abstract Introduction The worldwide shortage of donor livers has prompted the search for alternative cell therapies. Previous data from our laboratory proved a supportive role stem therapy in treatment end-stage liver disease patients. Therefore; this study was conducted to assess clinical and biochemical effects repeated infusion. Methods Ninety patients with cirrhosis were randomized receive either one session (G-I) or two sessions 4 months apart (G-II) autologous haematopoietic cells...
Objective To evaluate the antioxidant and apoptotic inductive effects of Withania somnifera (Ashwagandha) leaf extract against a hepatocellular carcinoma cell line. Methods After treating HepG2cells with Ashwagandha water (ASH-WX; 6.25 mg/ml-100 mg/ml), proliferation was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Antioxidant activities (total antioxidant, glutathione S-transferase reductase), Fas-ligand level, tumour necrosis factor-α (TNF-α)...
Background: Global DNA methylation has an impact in cancer pathogenesis and progression. This study aimed at investigating the of global treatment outcome Colorectal Cancer (CRC). Patients Methods: was measured by LC/MS/MS peripheral blood leucocytes 102, 48, 32 Egyptian CRC patients baseline after 3 6 months Fluoropyrimidine (FP) therapy respectively, addition to normal healthy matched age sex. The genetic expressions methyl transferases (DNMTs) were determined correlated with patients'...
Abstract The progression of the SARS-CoV-2 pandemic in Africa has so far been heterogeneous and full impact is not yet well understood. Here, we describe genomic epidemiology using a dataset 8746 genomes from 33 African countries two overseas territories. We show that epidemics most were initiated by importations, predominantly Europe, which diminished following early introduction international travel restrictions. As progressed, ongoing transmission many increasing mobility led to emergence...
Abstract Background Rapid pulmonary replication of SARS-CoV-2 can potentially trigger a strong immune response. In people with coronavirus disease 2019 (COVID-19) symptoms, cytokine storm syndrome often leads to acute respiratory distress and failure, which are key causes mortality. Viral infections activate both the innate adaptive systems, cellular response particularly role T lymphocytes being crucial for actual antiviral defense beside COVID-19. Among variants SARS-CoV-2, D614G mutation...
Abstract Background Genetic profiling of the tumor microenvironment involves looking at a lot genes to figure out what roles they play in progression cancer and how well treatments work. The is responsible for genesis, metastasis, resistance standard therapy. Characterizing through gene expression, such as ABCB1, FOXA, SFRP1, EGF, XPB1, can provide valuable insights into mechanisms driving behavior. By understanding interactions between these their surrounding environment, researchers...
There are eight genotypes of hepatitis B virus (A-H) and subgenotypes recognized. Genotyping can be accomplished based on a partial sequence HBV genome such as the pre-S or S gene. Several methods have been developed used for genotyping. This study was undertaken to determine in Egyptian pediatric cancer patients with acute chronic liver disease. were determined 22 who had forms disease (AH) 48 active (CAH). A type-specific primer nested-PCR method employed showed that infections attributed...
Human papillomavirus (HPV) 16 and 18 are associated with cervical carcinogenesis through an interaction between HPV oncogenic proteins cell cycle regulatory genes. However, the exact pathogenetic mechanisms not determined yet.We investigated 43 invasive squamous carcinoma (ISCC), 38 CIN III, 11 CINII CINI for cyclin D1, E, CDK4, p53, mdm-2, p21(waf), p27, p16(INK4A), Rb Ki-67 aberrations using immunohistochemistry molecular techniques. Twenty samples of normal tissues (NCT) were taken as a...
Occult HBV infection accelerates the progression of liver fibrosis, cirrhosis, and finally leading to hepatocellular carcinoma (HCC). This study analyzed occult HBV-genotypes in HCC patients.To achieve our objective, matched serum tissue samples were collected from 40 patients. Three sets primers used for HBV-DNA detection by nested-PCR, which cover HBV-genome; Core, Surface X genes. Genotyping system based on PCR using type-specific was applied positive samples.Intrahepatic detected 62.5%,...
Liver disease progression from chronic hepatitis C virus (HCV) infection to hepatocellular carcinoma (HCC) is associated with an imbalance between T-helper 1 and 2 cytokines. Evaluation of cytokines as possible candidate biomarkers for prediction HCC was performed using soluble Fas(sFas), tumor necrosis factor receptor-II (sTNFR-II), interleukin-2 receptor (IL-2R) interleukin-8 (IL-8).The following patients were recruited: 79 HCV infection, 30 HCC, 32 liver elevated enzyme levels (with or...
We studied promoter methylation (PM) of 11 genes in Peripheral Blood Lymphocytes (PBLs) and tissues hepatitis C virus (HCV) associated hepatocellular carcinoma (HCC) chronic (CH) Egyptian patients. The present study included 31 HCC with their ANT, 38 CH 13 normal hepatic tissue (NHT) samples. In all groups, PM APC, FHIT, p15, p73, p14, p16, DAPK1, CDH1, RARβ, RASSF1A, O(6)MGMT was assessed by methylation-specific PCR (MSP). APC O6-MGMT protein expression immunohistochemistry (IHC) the (20...
Background: Changes in DNA methylation patterns are believed to be early events hepatocarcinogenesis. A better understanding of states and how they correlate with disease progression will aid finding potential strategies for detection HCC. The aim our study was analyze the frequency tumor suppressor genes, P14, P15, P73, a mismatch repair gene (O6MGMT) HCV related chronic liver HCC identify candidate epigenetic biomarkers prediction. Materials Methods: 516 Egyptian patients HCV-related were...