Adrienne Amuri-Aziza
A5089854317- Poxvirus research and outbreaks
- Bacillus and Francisella bacterial research
- Herpesvirus Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- Rabies epidemiology and control
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Outbreaks Research
- vaccines and immunoinformatics approaches
- Zoonotic diseases and public health
- COVID-19 epidemiological studies
- Viral Infections and Immunology Research
- Vibrio bacteria research studies
- Virology and Viral Diseases
- Plant Virus Research Studies
- Respiratory viral infections research
- Influenza Virus Research Studies
- Disaster Response and Management
- Immune responses and vaccinations
- Hepatitis B Virus Studies
- Viral Infections and Vectors
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
National Institute of Biomedical Research
2021-2025
University of Kinshasa
2021-2023
SARS-CoV-2 across Africa The impact of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has been hard to track in African countries, largely because patchy data. Wilkinson et al . curated viral genomes collected 2021 from several countries continent. Outbreaks during 2020 each country were initiated by imported cases, mostly Europe. As developed, case numbers likely many times higher than reported, and subsequent waves appear have more severe. Consequently,...
Outbreaks of monkeypox (mpox) have historically resulted from zoonotic spillover clade I virus (MPXV) in Central Africa and II MPXV West Africa. In 2022, subclade IIb caused a global epidemic linked to transmission through sexual contact. Here we describe the epidemiological genomic features an mpox outbreak mining region eastern Democratic Republic Congo, by MPXV. Surveillance data collected between September 2023 January 2024 identified 241 suspected cases. Genomic analysis demonstrates...
Investment in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing Africa over the past year has led to a major increase number of sequences that have been generated and used track pandemic on continent, now exceeds 100,000 genomes. Our results show an African countries are able sequence domestically highlight local enables faster turnaround times more-regular routine surveillance. Despite limitations low testing proportions, findings from this genomic surveillance study...
ABSTRACT Background Monkeypox virus (MPXV) attracted global attention in 2022 during a widespread outbreak linked primarily to sexual contact. Clade I MPXV is prevalent Central Africa and characterized by severe disease high mortality, while II confined West associated with milder illness. A IIb emerged Nigeria 2017, protracted human-to-human transmission forerunner of the B.1 lineage 2022. In October 2023, large mpox Kamituga mining region Democratic Republic Congo (DRC), which we conducted...
Recent reports raise concerns on the changing epidemiology of mpox in Democratic Republic Congo (DRC). High-quality genomes were generated for 337 patients from 14/26 provinces to document whether increase number cases is due zoonotic spillover events or viral evolution, with enrichment APOBEC3 mutations linked human adaptation. Our study highlights two patterns transmission contributing source cases. All new sequences eastern South Kivu province (n = 17; 4.8%) corresponded recently...
Abstract Distinct SARS-CoV-2 lineages, discovered through various genomic surveillance initiatives, have emerged during the pandemic following unprecedented reductions in worldwide human mobility. We here describe a lineage - designated B.1.620 Lithuania and carrying many mutations deletions spike protein shared with widespread variants of concern (VOCs), including E484K, S477N HV69Δ, Y144Δ, LLA241/243Δ. As well as documenting suite this carries, we also its potential to be resistant...
ABSTRACT Background Recent reports raise concerns on the changing epidemiology of mpox in Democratic Republic Congo (DRC), with increasing case counts, sexual contact-mediated clusters, and sustained human-to-human transmission driven by a novel monkeypox virus (MPXV) subclade, clade Ib. However, only limited number I MPXV genomes have been characterized so far, from regions. Methods We conducted whole genome sequencing 603 mpox-positive samples that were collected 581 patients between...
BackgroundThe Democratic Republic of the Congo has had 15 Ebola virus disease (EVD) outbreaks, from 1976 to 2023. On June 1, 2020, declared an outbreak EVD in western Équateur Province (11th outbreak), proximal 2018 Tumba and Bikoro concurrent with eastern Nord Kivu Province. In this Article, we assessed whether 11th was genetically related previous or outbreaks connected available epidemiological genetic data identify sources possible zoonotic spillover, uncover additional unreported cases...
Between January and August 2024, mpox cases have been reported in nearly all provinces of the Democratic Republic Congo (DRC). Monkeypox virus genome sequences were obtained from 11 cases’ samples, collected July–August 2024 several health zones Kinshasa. Characterisation showed subclades Ia Ib co-circulating Limete zone, while phylogenetic analyses suggested multiple introductions two This illustrates growing complexity Clade I outbreaks DRC.
ABSTRACT The ongoing national mpox outbreak in the Democratic Republic of Congo has resulted more >30,000 suspected cases country from January 2023 to August 2024. While these historic case totals have been driven by primarily zoonosis, emergence Clade Ib monkeypox virus (MPXV), which is connected sustained human-to-human transmission, associated with increasing public health impacts eastern DRC. First identified South Kivu province, MPXV multiple non-endemic East African countries for...
We linked 4 mpox cases in South Ubangi, Democratic Republic of the Congo, to transboundary transmission from Central African Republic. Viral genome sequencing demonstrated that monkeypox virus sequences belonged distinct clusters subclade Ia. This finding demonstrates borderless nature and highlights need for vigilant regional surveillance.
Abstract The progression of the SARS-CoV-2 pandemic in Africa has so far been heterogeneous and full impact is not yet well understood. Here, we describe genomic epidemiology using a dataset 8746 genomes from 33 African countries two overseas territories. We show that epidemics most were initiated by importations, predominantly Europe, which diminished following early introduction international travel restrictions. As progressed, ongoing transmission many increasing mobility led to emergence...
Timely detection of outbreaks is needed for poliovirus eradication, but gold standard in the Democratic Republic Congo takes 30 days (median). Direct molecular and nanopore sequencing (DDNS) stool samples a promising fast method. Here we report prospective testing from suspected polio cases, their contacts, between 10 August 2021 4 February 2022. DDNS detected polioviruses 62/2,339 (2.7%) samples, while combination cell culture, quantitative PCR Sanger 51/2,339 (2.2%) same samples. provided...
Human Mpox cases are increasingly reported in Africa, with the highest burden Democratic Republic of Congo (DRC). While case reporting on a clinical basis can overestimate infection rates, laboratory confirmation by PCR underestimate them, especially suboptimal samples like blood, commonly used DRC. Here we Luminex-based assay to evaluate whether antibody testing be complementary confirm and identify human transmission chains during outbreak investigations. We left-over blood from 463...
ABSTRACT Cholera remains a significant public health burden in many countries sub-Saharan Africa, though the exact mechanisms of bacterial emergence and spread remain largely undefined. We generated genomic data from 728 Vibrio cholerae O1 isolates predominantly collected between 2019-2024 to create largest dataset V. genomes sequenced locally Africa. This enabled us interrogate recent patterns spread, including rapid circulation AFR15 lineage associated with unusually large outbreaks...
Summary Background One year after the coronavirus disease 2019 (COVID-19) pandemic, focus of attention has shifted to emergence and spread severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants concern (VOCs). The aim study was assess frequency VOCs in patients followed for COVID-19 at Kinshasa university hospital (KUH) during 3rd 4th waves pandemic Kinshasa. Hospital mortality compared that first two waves. Method present included all whom diagnosis SARS-CoV-2 infection...
Abstract Investment in Africa over the past year with regards to SARS-CoV-2 genotyping has led a massive increase number of sequences, exceeding 100,000 genomes generated track pandemic on continent. Our results show an African countries able sequence within their own borders, coupled decrease sequencing turnaround time. Findings from this genomic surveillance underscores heterogeneous nature but we observe repeated dissemination variants Sustained investment for is needed as virus continues...
ABSTRACT Four confirmed mpox cases in South Ubangi province, Democratic Republic of the Congo, were linked to documented transboundary transmission from Central African Republic. Viral genome sequencing shows that MPXV sequences belong subclade Ia. This demonstrates borderless nature and highlights need for vigilant regional surveillance.
During the 2018-20 Ebola virus disease outbreak in Democratic Republic of Congo, thousands patients received unprecedented vaccination, monoclonal antibody (mAb) therapy, or both, leading to a large number survivors. We aimed report clinical, virological, viral genomic, and immunological features two previously vaccinated mAb-treated survivors Congo who developed second episodes months after initial discharge, ultimately complicated by fatal meningoencephalitis associated with persistence.
Abstract Mpox cases have been reported in nearly all provinces of the Democratic Republic Congo as August 2024. Monkeypox virus positive samples from Kinshasa, collected between July and mid-August 2024, were sequenced using a probe-based enrichment or optimized tiling sequencing protocol. With multiple introductions both Clade Ia (7/12) Ib (5/12), marking its Limete health zone specifically, an area with co-circulation I, unique observation illustrating growing complexity I mpox outbreaks DRC.
ABSTRACT Background Monkeypox virus (MPXV) is endemic to the Democratic Republic of Congo (DRC), and historically has been reported in rural, forested regions with limited household primarily zoonotic transmission. With emergence subclade Ib increasing human-to-human transmission MPXV we sought characterize both epidemiologic genomic characteristics confirmed mpox cases Kinshasa, DRC, following introduction August 2023. Methods This retrospective observational study leveraged demographic...
Several mpox outbreaks, caused by subclades Ia and Ib monkeypox virus (MPXV), are ongoing in the Democratic Republic of Congo. The World Health Organization declared outbreak a Public Emergency International Concern due to geographic expansion subclade Ib. We assessed performance novel multiplex real-time PCR assay designed for MPXV detection simultaneous identification. This demonstrates high accuracy specificity, underscoring its use current outbreak.
Abstract Delayed detection of poliovirus outbreaks is a major threat to polio eradication. Direct molecular Detection and Nanopore Sequencing (DDNS) stool samples shows promise as faster method detect confirm cases compared with cell culture but has not been assessed prospectively during routine surveillance. We report on the implementation prospective testing all received from suspected their contacts in Democratic Republic Congo between 10 th August 2021 4 February 2022. DDNS detected...