A5043967357- Laser-induced spectroscopy and plasma
- Protein Hydrolysis and Bioactive Peptides
- Bacterial Genetics and Biotechnology
- Effects of Radiation Exposure
- Peptidase Inhibition and Analysis
- Probiotics and Fermented Foods
- Mass Spectrometry Techniques and Applications
- Analytical chemistry methods development
- Carcinogens and Genotoxicity Assessment
- Genetic diversity and population structure
- Environmental DNA in Biodiversity Studies
- RNA and protein synthesis mechanisms
- Risk Perception and Management
- Cultural Heritage Materials Analysis
- Ion-surface interactions and analysis
- Species Distribution and Climate Change
- Antimicrobial Resistance in Staphylococcus
- Polyamine Metabolism and Applications
- Cancer Cells and Metastasis
- Identification and Quantification in Food
- Cell Image Analysis Techniques
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Electrochemical Analysis and Applications
- Oral microbiology and periodontitis research
Université Claude Bernard Lyon 1
1990-2023
Centre National de la Recherche Scientifique
1990-2023
École Nationale des Travaux Publics de l'État
2023
Institut de Radioprotection et de Sûreté Nucléaire
2008-2022
Inserm
2012
Laboratoire de Microbiologie et Génétique Moléculaires
1991-2006
Centre de Génétique Moléculaire
1996
Bacterial samples (Escherichia coli and Bacillus subtilis) have been analyzed by laser-induced breakdown spectroscopy (LIBS) using femtosecond pulses. We compare the obtained spectra with those resulting from classical nanosecond LIBS. Specific features of LIBS demonstrated, very attractive for analyzing biological sample: (i) a lower plasma temperature leading to negligible nitrogen oxygen emissions excited ambient air better contrast in detection trace mineral species; (ii) specific...
A sample of Escherichia coli has been analyzed by laser-induced breakdown spectroscopy (LIBS) using femtosecond pulses. The spectrum shows strong CN molecular bands due to the direct ablation native bonds from bacteria in contrast with weak atomic lines carbon. nature observed is supported kinetic behavior band head which rapidly decays a time constant 94 ns, while for pure graphite increases delay 450 ns recombination ambient air. Moreover, about hundred resolved belonging 12 or species are...
Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Twitter Facebook Reddit LinkedIn Tools Reprints and Permissions Cite Search Site Citation Matthieu Baudelet, Jin Yu, Myriam Bossu, Julien Jovelet, Jean-Pierre Wolf, Tanguy Amodeo, Emeric Fréjafon, Patrick Laloi; Discrimination of microbiological samples using femtosecond laser-induced breakdown spectroscopy. Appl. Phys. Lett. 16 October 2006; 89 (16): 163903. https://doi.org/10.1063/1.2361270...
Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397 is able to hydrolyze X-proline- para -nitroanilides and X-proline-β-naphthylamides (X for alanyl- or glycyl-). A single metal-independent cytoplasmic enzyme with a molecular weight estimated be 82,000 responsible these activities was named X-prolyl-dipeptidyl aminopeptidase (X-Pro-DPAP). Isolation analysis of mutants totally deficient X-Pro-DPAP activity showed that total lack this induces (i) decrease in the growth rate; (ii) an increase...
Lactobacillus bulgaricus CNRZ 397 is able to hydrolyze many amino-acyl- and dipeptidyl-β-naphthylamides. Analysis of heat inactivation kinetics, protease inhibitor effects, the subcellular location aminopeptidase (AP) activities from parental strain mutant derivatives dificient in alanyl- or leucyl-β-naphthylamide hydrolysis pointed out existence four APs. All mutants isolated were totally deficient AP II, a cell wall metallo-enzyme with broad substrate specificity but that specifically...
Reproductive effects of ionizing radiation in organisms have been observed under laboratory and field conditions. Such assessments often rely on associations between exposure effects, thus lacking a detailed mechanistic understanding causality occurring at different levels biological organization. The Adverse Outcome Pathway (AOP), conceptual knowledge framework to capture, organize, evaluate visualize the scientific relevant toxicological has potential causal relationships molecular,...
Despite the ubiquity of pollutants in environment, their long-term ecological consequences are not always clear and still poorly studied. This is case concerning radioactive contamination environment following major nuclear accident at Chernobyl power plant. Notwithstanding implications evolutionary processes on population status, few studies concern evolution organisms chronically exposed to ionizing radiation exclusion zone. Here, we examined genetic markers for 19 populations Eastern tree frog (
ABSTRACT The porin composition of the Escherichia coli cell envelope was analyzed during growth at different external pHs (pHo) as a function acetyl phosphate (AcP) level (Δ ackA pta or mutant, pyruvate glucose carbon source) in presence absence EnvZ. Our results indicate that AcP is influenced by pHo, leading to modulation amount OmpR-P and subsequent pHo-dependent expression ompF ompC . We also propose existence specific signal, independent EnvZ AcP, OmpR phosphorylation response pyruvate.
Summary: Lactobacillus helveticus ITGL1 is able to hydrolyse many amino-acyl and dipeptidyl-p-nitroanilides. Analysis of heat inactivation kinetics, metal ion protease inhibitor effects, the subcellular location aminopeptidase activities in both parental strain mutants deficient lysyl-p-nitroanilide hydrolysis, led characterization two cell-wall-associated aminopeptidases. APII APIV. APII, which catalysed L-lysine p-nitroanilide was purified about 28-fold homogeneity from cell-wall extracts...
Glucosyltranferases (Gtfs) produced by the mutans streptococci are recognized as virulence factors in dental caries, and inhibition of Gtfs secretory immunoglobulin A is predicted to provide protection against this disease. The basis such mucosal immunity linked ability reliably stimulate production Gtfs. In regard, we exploring immunogenicities various Gtf peptides genetically fused B subunit cholera toxin (CTB), a known adjuvant. work, have created gene fusion linking GtfB active-site (AS)...
ABSTRACT The expression of the maltose system in Escherichia coli is regulated at both transcriptional and translational levels by pH growth medium (pHo). With glycerol as carbon source, transcription malT , encoding activator regulon, weaker acidic than alkaline medium. became high, regardless pHo, when glycerol-3-phosphate or succinate was used source. Conversely, low, increase transcription, associated with requires presence kinase (GlpK). Changes level glpK had a great effect on...
In Escherichia coli membranes, three major phospholipids are formed: phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and cardiolipin (CL). We report here the survival of mutants lacking either PE or both PG CL at an acid pHo during long-term experiments. Stationary phase cultures E. much more sensitive to shock (pHo 3) than wild-type strain. Moreover, in strain PE, stationary is impaired after 5 days no viable cells recovered. The exponential culture known be increased if exposed...
Summary: The Escherichia coli maltose regulon consists of five operons under the control MalT transcriptional activator. lac operon fusions were constructed in vitro with MalT-dependent promoter and malT itself. -Galactosidase activity displayed by these during growth at different external pH (pH o ) revealed that a higher than 6 stimulates transcription - MalT-controlled genes absence or presence maltose. Using malTp1 malTp10 is cAMP-CRP (cAMP receptor protein)-independent, it was...
Laser Induced Breakdown Spectroscopy spectra of bacteria, with nanosecond and femtosecond ablation, are compared. High sensitivity for mineral trace detections, larger intensity from molecular bands precise kinetic study among benefits using short pulses.
Journal Article Review of epidemiology studies childhood leukaemia near nuclear facilities: answer to the commentary from Fairlie and Körblein Get access Dominique Laurier, Laurier Search for other works by this author on: Oxford Academic PubMed Google Scholar Sophie Jacob, Jacob Patrick Laloi Institute Radiological Protection Nuclear Safety Fontenay aux RosesFrance Radiation Dosimetry, Volume 138, Issue 2, February 2010, Pages 195–197, https://doi.org/10.1093/rpd/ncp247 Published: 05 November 2009
Laurier, Dominique; Grosche, Bernd; Jacob, Sophie; Sermage-Faure, Claire; Laloi, Patrick; Tirmarche, Margot; Hémon, Denis; Clavel, Jacqueline Author Information
Memory, learning and sensorimotor are govern by cerebral cortex which is a cholinergic widespread structure. Previous studies showed that sleep/wake cycle, spatial memory locomotion were impaired after chronic exposure to depleted uranium (DU) enriched (EU). The aim of this study was establish if these physiological disruptions came from system alteration. Rats ingested DU or EU drinking water at 40 mg/L during 1.5 9 months. Cortex removed then gene expression protein level analysed assess...