Coronary artery disease (CAD) is a major cause of death all over the world. CAD caused by atherosclerosis which induced interaction genetic factors and environmental factors. Traditional risk include hyperlipidemia, diabetes mellitus, lack exercise, obesity, poor diet others. Genome-wide association studies have revealed certain gene polymorphisms with susceptibility to CAD. Omentin 1 an adipokine secreted visceral adipose tissues has been reported anti-inflammatory, cardioprotective,...

Non-small cell lung cancer has a devastating prognosis, and markers enabling precise prediction of therapy response have long remained scarce. Better treatment monitoring would allow an individual's more effective patient adjusted with lesser side effects good clinical outcomes. In the present study, we monitored serum cytochrome c levels pre- post-chemotherapy non-small patients. Using highly sensitive enzyme-linked immunosorbent assay, evaluated in 100 healthy controls. We observed about...

Dysregulation in the miRNA-21 expression has been previously observed a number of malignancies and not only tumor cell itself but also body fluids cancer patients. The present study aimed to find out clinical significance cell-free circulating as an efficient non-invasive biomarker for screening lung case-control included plasma samples from 80 patients healthy controls. Magnetic bead technology was used miRNA isolation advanced TaqMan assays were quantification level individuals.The overall...

Studies have evaluated the association of miRNA-423 C>A genotyping with susceptibility to various diseases such cancers, atherosclerosis and inflammatory bowel disease but results were contradictory. However, no studies reported between rs6505162 polymorphism coronary artery disease. MicroRNAs regulate expression multiple genes involved in atherogenesis. Therefore, we investigated microRNA-423C>T gene variations disease.This study was conducted on 100 patients 117 matched healthy controls....

Background: Epithelial ovarian cancer continues to be a deleterious threat women as it is asymptomatic and typically detected in advanced stages. Cogent non-invasive biomarkers are therefore needed which effective apprehending the disease early Recently, miRNA deregulation has shown promising magnitude tumorigenesis. miRNA-145(miR- 145) beginning understood for its possible role development progression. In this study, we identified clinicopathological hallmarks altered owing downexpression...

Chronic myeloid leukemia (CML) is characterized by the Philadelphia chromosome, an abnormally shortened chromosome 22. It result of a reciprocal translocation chromosomes 9 and 22, creating BCR-ABL fusion transcripts, b3a2, b2a2, e1a2. The aim our study was to determine type transcripts for molecular diagnosis investigate frequency in CML patients multiplex RT-PCR CML.A single reaction with multiple primers PCR used detect 200 among which 116, 33, 51 were CP, AP, BC phase, respectively.The...

MDM2 protein is an important regulator of the p53 pathway and has a large effect on anti-tumorigenic activity p53. Presently, we aimed to analyze possible association mdm2 in development progression non-small cell lung cancer. In addition, impact gene promoter polymorphism (T309G, rs 2279744) its expression ultimately TP53 was investigated cancer patients. A case–control study using peripheral blood samples 100 patients free healthy controls conducted. Expression profile were evaluated by...

Genetic variants in pre-microRNA genes or the 3'UTR of miRNA target could influence miRNA-mediated regulation gene expression and thus contribute to susceptibility prognosis human diseases. Several studies have investigated association genetic seed region miRNAs with cardiometabolic phenotypes .Therefore aim study was investigate potential miR-4513 rs2168518 C>T variability risk developing CAD its different an Indian cohort stratify burden general population.The conducted on 100 clinically...

To investigate the methylation pattern in promoter region of RASSF1A and PTEN genes epithelial ovarian cancer patients North India. Fifty 20 healthy controls were studied. Isolation genomic DNA from peripheral blood methylation-specific polymerase chain reaction (MSP) applied for analysis. 17 50 (34.0%) found to be methylated gene, whereas gene occurred 8 cases (16.0%). A statistically significant result was obtained (P = .01) correlated with patients' clinicopathologic features....

Background: Cardiovascular diseases (CVD) are important causes of death worldwide. Atherosclerosis is a chronic inflammatory disorder. It the major cause CVD and manifested by ischemic heart disease or coronary artery (CAD). TNF-α pro-inflammatory cytokine that regulates immune response promotes development atherosclerosis. Cytochrome p450 1B1 (CYP1B1) an enzyme involved in metabolism endogenous exogenous substrates. Objectives: This study aimed at examining association rs1800629 G>A...

Survivin is highly expressed in fetal tissue and completely absent terminally differentiated cells, but its re-expression has been observed most human tumors. Presently, we aimed to analyze the possible impact of survivin gene (-31G > C, rs 9904341) promoter polymorphism on expression profile ultimately role development progression non-small cell lung cancer. A case-control study 100 cancer patients cancer-free healthy controls was conducted. analyzed by PCR-restriction fragment length...

Aim: Apart from the modifiable risk factors, genetic factors are believed to influence outcome of Coronary Artery Diseases (CAD). Under miRNA polymorphisms, namely Hsa-miR-146a-5p (rs2910164) have become an important tool study mechanism that underlies pathogenesis this disease. Therefore, we investigated association miR-146a gene variations with susceptibility coronary artery diseases. Methodology: This was conducted on 100 CAD patients and 117 matched healthy individuals. Genotyping C>G...

Purpose: Catechol-O-methyltransferase (COMT) plays a central role in DNA repair and estrogen-induced carcinogenesis. The nonsynonymous single nucleotide polymorphism (SNP) exon 4 G > A or Val108 158Met rs4680 influences COMT enzyme activity. three phenotypes of the activities include A/A with low activity, A/G medium activity G/G high Met allele is associated enzymatic resulting higher levels prefrontal dopamine. Conversely, Val lower has been several psychiatric disorders such as panic...

Coronary artery disease (CAD) is an important cause of death worldwide. CAD caused by genetic and other factors including hypertension, hyperlipidemia, obesity, stress, unhealthy diet, physical inactively, smoking Type 2 diabetes (T2D). The genome wide association studies (GWASs) have revealed the many loci with risk to diseases such as cancers, T2D CAD. Nitric oxide (NO) a potent vasodilator required for normal vascular health. It produced in endothelial cells reaction catalyzed NO synthase...

Coronary artery disease (CAD) is the leading cause of death and hospitalization worldwide represents a problem for public health systems everywhere. In Saudi Arabia, prevalence CAD estimated to be 5.5%. Risk factors include older age, male gender, obesity, high blood pressure, smoking, diabetes, hyperlipidemia, genetic factors. Reducing risk in susceptible individuals will decrease CAD. Genome wide association studies have helped reveal many loci with diseases like this study, we examined...

Purpose: The impact of the BRCA1-3’UTR-variant on BRCA1 gene expression and altered responses to external stimuli was previously tested in vitro using a luciferase reporter assay. Its ability predict breast cancer risk women also assessed but conclusions were inconsistent. present study concerns relationship between BRCA1-3’UTR germline variant rs8176318G>T susceptibility Breast an ethnic population Saudi Arabia. Methodology: included 100 patients sex matched healthy controls from...

Background: The epidermal growth factor (EGF) plays important roles in non-small cell lung cancer (NSCLC) susceptibility and functional polymorphism the EGF (+61A/G) gene has been linked to increased risk of NSCLC. This study aimed evaluate role +61A/G NSCLC adenocarcinoma (ADC) occurrence survival an Indian population. Materials Methods: casecontrol included 100 histopathologically confirmed patients healthy controls. (A61G) was genotyped by AS-PCR elucidate putative associations with...

Downregulation of CASP3 gene expression has been observed to be associated with various malignancies, and promoter polymorphisms in the may have a great impact on transcriptional activity. The present study aimed analyze possible (-1337 C > G, rs1405937) polymorphism profile ultimately its association development non-small cell lung cancer. A case-control 100 cancer patients free healthy controls was conducted, wherein genotype were evaluated using serum DNA RNA, respectively, by...

NSCLC is a disease involving uncontrolled cell growth, which could result in metastases into nearby tissues beyond the lungs.The aim of present study was to analyze influence epidermal growth factor receptor (EGFR) gene expression on metastasis and survival patients. The case-control included 100 cases patients age sex matched controls. EGFR analyzed by quantitative real time PCR using serum RNA. Association with patient Kaplan-Meier method.We observed mean increased 13.5 fold Values...

Serum messenger RNA (mRNA) is an emerging prognostic tool for noninvasive malignant disease prognosis, and to study serum mRNA may have importance in the prognosis detection of disease. This aimed evaluate possible role ERBB3 ERBB4 expressions lung adenocarcinoma patients. One hundred newly diagnosed patients 100 age- sex-matched healthy controls were included. Expression was analysed by quantitative real-time PCR overall survival Kaplan-Meier analysis. found be significantly associated with...

The microRNAs regulate the expression of multiple genes involved in diseases such as cancer, diabetes and cardiovascular disease. In this study, we have investigated association between miR-224 gene polymorphism (rs188519172A>G) susceptibility coronary artery disease CAD.Hundred CAD patients 100-matched healthy control were included. Genotyping was performed using Amplification refractory mutation system PCR method (ARMS-PCR).A significant difference observed genotype distribution among...