A5029599049- Chronic Myeloid Leukemia Treatments
- Monoclonal and Polyclonal Antibodies Research
- Acute Myeloid Leukemia Research
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related Molecular Pathways
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- MicroRNA in disease regulation
- Protein Kinase Regulation and GTPase Signaling
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Kruppel-like factors research
- Chronic Lymphocytic Leukemia Research
- Acute Lymphoblastic Leukemia research
- Ubiquitin and proteasome pathways
- Eosinophilic Disorders and Syndromes
- Platelet Disorders and Treatments
- Neuroscience and Neuropharmacology Research
- Computational Drug Discovery Methods
- Chemical Synthesis and Analysis
- Cell Adhesion Molecules Research
- Epilepsy research and treatment
- Cancer-related gene regulation
- ATP Synthase and ATPases Research
- Peptidase Inhibition and Analysis
Medical University of South Carolina
2020-2023
MUSC Hollings Cancer Center
2020-2023
Ralph H. Johnson VA Medical Center
2020-2023
University of Illinois Chicago
2018-2020
Maulana Azad Medical College
2014-2020
Cancer Genetics (United States)
2015
MicroRNAs (miRNAs) have been found to be dysregulated in epithelial ovarian cancer (EOC) and may function as either tumor suppressor genes (TSGs) or oncogenes. Hypermethylation of miRNA silences the tumour suppressive a hypermethylation TSG regulating that (or vice versa) leads its loss function. The present study aims evaluate impact aberrant microRNA-125b (miR-125b) expression on various clinicopathological features association with anomalous methylation several TSGs. We enrolled 70 newly...
The G12D mutation is among the most common KRAS mutations associated with cancer, in particular, pancreatic cancer. Here, we have developed monobodies, small synthetic binding proteins, that are selective to KRAS(G12D) over KRAS(wild type) and other oncogenic mutations, as well HRAS NRAS. Crystallographic studies revealed that, similar mutant-selective inhibitors, initial monobody bound S-II pocket, groove between switch II α3 helix, captured this pocket widely open form reported date....
RAS mutants are major therapeutic targets in oncology with few efficacious direct inhibitors available. The identification of a shallow pocket near the Switch II region on has led to development small-molecule drugs that target this site and inhibit KRAS(G12C) KRAS(G12D). To discover other regions may be targeted for inhibition, we have employed small synthetic binding proteins termed monobodies strong propensity bind functional sites protein. Here, report pan-RAS monobody, JAM20, bound all...
RAS guanosine triphosphatases (GTPases) are mutated in nearly 20% of human tumors, making them an attractive therapeutic target. Following our discovery that nucleotide-free (apo RAS) regulates cell signaling, we selectively target this state as approach to inhibit function. Here, describe the R15 monobody exclusively binds apo all three isoforms vitro, regardless mutation status, and captures cells. inhibits signaling transforming activity a subset mutants with elevated intrinsic nucleotide...
Epithelial ovarian cancer (EOC) is the most lethal cause of morbidity and mortality worldwide. miRNA deregulation evinces a remarkable role in tumorigenesis. miRNA-199a (miR-199a) known to be involved development progression. Although miR-199a has been studied various cell types, its correlation with clinicopathological features EOC not documented. In this study, we identified hallmarks which might perturbed due downregulation serum EOC. Seventy samples from histopathologically confirmed...
Background: Epithelial ovarian cancer continues to be a deleterious threat women as it is asymptomatic and typically detected in advanced stages. Cogent non-invasive biomarkers are therefore needed which effective apprehending the disease early Recently, miRNA deregulation has shown promising magnitude tumorigenesis. miRNA-145(miR- 145) beginning understood for its possible role development progression. In this study, we identified clinicopathological hallmarks altered owing downexpression...
Chronic myeloid leukemia (CML) is characterized by the Philadelphia chromosome, an abnormally shortened chromosome 22. It result of a reciprocal translocation chromosomes 9 and 22, creating BCR-ABL fusion transcripts, b3a2, b2a2, e1a2. The aim our study was to determine type transcripts for molecular diagnosis investigate frequency in CML patients multiplex RT-PCR CML.A single reaction with multiple primers PCR used detect 200 among which 116, 33, 51 were CP, AP, BC phase, respectively.The...
is the most frequently mutated oncogene in human cancer with nearly ~20% of patients possessing mutations one three
To investigate the methylation pattern in promoter region of RASSF1A and PTEN genes epithelial ovarian cancer patients North India. Fifty 20 healthy controls were studied. Isolation genomic DNA from peripheral blood methylation-specific polymerase chain reaction (MSP) applied for analysis. 17 50 (34.0%) found to be methylated gene, whereas gene occurred 8 cases (16.0%). A statistically significant result was obtained (P = .01) correlated with patients' clinicopathologic features....
Background: Mammalian cells contain three functional RAS proto-oncogenes, known as H-RAS, K-RAS, and N-RAS, which encode small GTP-binding proteins in terms of p21 ras s. genes have been elucidated major participants the development progression cancer. A single nucleotide polymorphism (SNP) at H-RAS cDNA position 81 T→C (rs12628) has found to be associated with risk many human cancers like gastrointestinal, oral, colon, bladder thyroid carcinomas. Therefore, we hypothesized that this...
Background: Worldwide, breast cancer is the most common among women and a leading cause of death. In present study, we investigated NQO1 C609T genotypic allelic distribution in north Indian patients. Materials Methods: The NQ01 polymorphism was assessed 100 invasive ductal carcinoma (IDC) patients healthy controls using allele specific PCR (AS-PCR). Results: A lower frequency CC genotype found (24%) than controls. On other hand, TT also to be higher female (32%) (20%). frequencies all three...
RIZ1 encodes a retinoblastoma (Rb)-interacting zinc finger protein, is commonly lost or expressed at reduced levels in cancer cells. The gene locus undergoes LOH many cancers. Here, we analyzed Proline insertion–deletion polymorphism amino acid position 704 the and its association with CML. pro-704 genotypes were determined by AS-PCR 100 CML patients among which 50 CP-CML, 25 AP-CML, BC-CML. Pro704 ins/del (LOH) was detected 27% patients. ins–ins homozygosity, del–del homozygosity ins–del...
Background: TP53, located on chromosome 17p13, is one of the most mutated genes affecting many types human cancers .To establish an association between incidence exon 8 p53 (R282W) gene and progression disease in CML also to correlate presence mutation with clinicopathological features disease. Methods: status was investigated by studying mutations at region after confirming diagnosis BCR-ABL. 100 samples were analyzed using Allele-Specific Oligonucleotide PCR assay. Mutations occurred 58%...
Platelet-derived growth factor receptor has been implicated in many malignant and non-malignant diseases. receptor-α is a tyrosine kinase side target for imatinib, revolutionary drug the treatment of chronic myeloid leukemia that dramatically improved survival patients. Given importance platelet-derived platelet development its inhibition by it was intriguing to analyze role relation imatinib imatinib-induced thrombocytopenia We hypothesized two known functional polymorphisms, +68GA...
Introduction: Epilepsy is the most common heterogeneous neurological disorder affecting approximately 42 million people worldwide. Juvenile myoclonic epilepsy (JME) a form of idiopathic generalized representing 5-10% all cases. Lennox-Gastaut syndrome (LGS) one severe epileptic encephalopathies childhood onset, cause which may be symptomatic, i.e., secondary to an underlying brain or cryptogenic, with no known cause. Sodium channels are integral membrane proteins play central role in...
Abstract Aim: Lung cancer is considered to be the most common in world. In humans, about 50% or more cancers have a mutated tumor suppressor p53 gene thereby resulting accumulation of protein and losing its function activate target genes that regulate cell cycle apoptosis. Extensive research conducted murine models with activated p53, loss missense mutations facilitated researchers understand role this key protein. Our study was aimed evaluate frequency cytosine deletion nonsmall lung...