A5027975365- Aquaculture disease management and microbiota
- Animal Virus Infections Studies
- Viral Infections and Vectors
- Virology and Viral Diseases
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- Animal Disease Management and Epidemiology
- Biofuel production and bioconversion
- Viral Infections and Immunology Research
- Identification and Quantification in Food
- Marine Bivalve and Aquaculture Studies
- Botany and Plant Ecology Studies
- biodegradable polymer synthesis and properties
- Water Quality and Pollution Assessment
- Nanocomposite Films for Food Packaging
- Antimicrobial Peptides and Activities
- Pediatric health and respiratory diseases
- Microbial infections and disease research
- Biosensors and Analytical Detection
- Heavy metals in environment
- Plant Virus Research Studies
- Meat and Animal Product Quality
- Heavy Metals in Plants
- Energy Harvesting in Wireless Networks
Institut National des Sciences et Technologies de la Mer
2015-2025
Tunis El Manar University
2022-2025
Institut de Ciències del Mar
2024
Hôpital La Rabta
2009-2010
To detect the possible coexistence of striped jack nervous necrosis virus (SJNNV) and red-spotted grouper (RGNNV) genotypes in a single fish, methodology based on combination PCR amplification blot hybridization has been developed applied this study.The degenerate primers designed for procedure target T4 region within capsid gene, resulting both genotypes. The subsequent these products with two different specific digoxigenin-labelled probes resulted identification separately. application...
The main objectives of this work are: firstly, the study circulation Rotavirus A (RVA) in wastewater sampled from a semi‐industrial pilot plant. plant is situated residential area Tunis and irrigated by three different neighboring hospitals; secondly, evaluation functioning two biological treatment procedures (natural lagoons biodisks) for RVA removal using molecular characterization quantification technology. total 102 samples were collected processes that receives effluents sewage system...
The efficiency of rotating biodisks and natural oxidizing lagoon procedures is investigated at a Tunisian semi-industrial pilot plant, El Menzeh I, where the wastewater mainly provided by three different neighbouring hospital clinics. Throughout 2011, 102 samples were collected from two mentioned treatment procedures. Results showed that Sapovirus (SaV) frequency was approximately 29.4% using real-time reverse transcription polymerase chain reaction (RT-PCR) technique, about 16.6%...
Finfish nodaviruses (betanodaviruses) can cause highly destructive infections in numerous species of farmed marine fish larvae and juveniles worldwide. The betanodavirus genome consists two single-stranded positive-sense RNA molecules (RNA1 RNA2). virus be classified into four genotypes based on the partial sequences coat protein (CP) gene (T2 T4 regions). Currently, genomic sequence information for RNA1 regions RNA2 outside T2 is less well documented. This study reports characterization...
We describe the design of a simple and highly sensitive electrochemical bioanalytical method enabling direct detection conserved RNA region within capsid protein gene fish nodavirus, making use nanostructured disposable electrodes. To achieve this goal, we select nodavirus RNA2 segment to DNA probe that is tethered surface screen-printed In proof-of-principle test, synthetic sequence detected based on competitive hybridization between two oligonucleotides (biotinylated reporter target RNA)...
The effect of sunlight and salinities (10, 20, 39, 60 psu) on the survival Vibrio parahaemolyticus strains carrying either (thermostable direct hemolysin) tdh, related trh, both or none them were studied in water microcosms stabilized at 20°C using plate count agar acridine orange viable count. All V. exposed to rapidly lose their culturability evolve into a but non-culturable state (VBNC). However, tdh positive remain more culturable than non-virulent trh strain statically insignificant. At...
Viral Encephalopathy and Retinopathy (VER), is caused by a nodavirus included within the Betanodavirus genus of Nodaviridae family. This disease affects more than 30 marine fish species worldwide has been major obstacle in aquaculture industry; control based on virus detection, essentially carrier specimens. study describes real time PCR procedure for viral nervous necrosis detection from several organs sea bass, Senegalese sole, gilt-head bream, displaying either clinical symptoms or...
The results of the present study describe molecular identification Lymphocystis Disease Viral partial genome (LCDV-Sa) and histopathology in skin nodules internal organs Tunisian gilthead sea bream (<em>Sparus aurata</em>)..
The purpose of this study was to determine the phylogenetic relationships among primary betanodavirus strains circulating in Tunisian coastal waters. A survey conducted investigate nodavirus infections at 15 European sea bass Dicentrarchus labrax and gilthead bream Sparus aurata farming sites located along northern eastern coasts Tunisia. objective create epidemiological awareness these by determining between main during period 2012-2019, using RNA1 and/or RNA2 genome segments. Approximately...
<title>Abstract</title> Analysing animal responses to immunization is pivotal in vaccine development by evaluating immune response, assessing safety and efficacy, providing crucial insights into protection mechanisms. These are indispensable for advancing vaccines through trial stages regulatory approval processes, as well deciphering the molecular signatures of approved vaccines, which not only enhances our understanding existing but also informs rational design new ones. This study aims...
Abstract R.A73 Enterococcus faecium (HG937697) was tested for investigating anti-nodaviral activity and immunomodulatory properties on E-11 fish cells. Non R.A73-related toxicity confirmed. Likewise, in-vitro inhibition of nodavirus replication gene expression capsid protein in addition to CCR3, IL-10 TNFα genes were evaluated using qPCR. A preventive treatment cells by the bacterial suspension generated a remarkable reduction virus loads (RQ values from 0.1 0.9) at day 7. Interestingly, E....