A5030993577- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Psoriasis: Treatment and Pathogenesis
- Rheumatoid Arthritis Research and Therapies
- Spondyloarthritis Studies and Treatments
- Neurogenetic and Muscular Disorders Research
- Aquaculture disease management and microbiota
- Helminth infection and control
- Neuroscience and Neural Engineering
- Viral Infections and Vectors
- Ion channel regulation and function
- Microfluidic and Capillary Electrophoresis Applications
- DNA and Nucleic Acid Chemistry
- Parasite Biology and Host Interactions
- CRISPR and Genetic Engineering
- Animal Disease Management and Epidemiology
- Neonatal Respiratory Health Research
- Vector-Borne Animal Diseases
- Analytical Chemistry and Sensors
- Connective tissue disorders research
- Bird parasitology and diseases
Tunis El Manar University
2016-2023
Institut Pasteur de Tunis
2018-2023
Tunis University
2009-2021
Faculté de médecine de Tunis
2009
This paper describes a disposable amperometric biosensor for simple and sensitive determination of miRNA-21. The bioplatform consists gold nanoparticles-nanostructured electrode surfaces on which direct hybridization assay involving the immobilization specific thiolated DNA capture probe recognition formed DNA–miRNA-21 heteroduplex by antibody is implemented. further recognized through its Fc region commercial bacterial protein containing 40 units horseradish peroxidase (HRP)...
This work reports an amperometric biosensor for the determination of miRNA-21, a relevant oncogene. The methodology involves competitive DNA-target miRNA hybridization assay performed on surface magnetic microbeads (MBs) and transduction at screen-printed carbon electrodes (SPCEs). target competes with synthetic fluorescein isothiocyanate (FITC)-modified identical sequence biotinylated complementary DNA probe (b-Cp) immobilized streptavidin-modified MBs (b-Cp-MBs). Upon labeling,...
Epizootic haemorrhagic disease virus (EHDV) was detected for the first time in Tunisia and other Northern African countries 2006. The objective of present study to investigate whether EHDV circulated Tunisian livestock before after officially-reported outbreak Thus, serum samples from cattle dromedaries collected different periods (before 2006) regions were screened presence antibodies. Serological investigations conducted on dromedary sera 2000 2001 demonstrated no circulation these dates....
We describe the design of a simple and highly sensitive electrochemical bioanalytical method enabling direct detection conserved RNA region within capsid protein gene fish nodavirus, making use nanostructured disposable electrodes. To achieve this goal, we select nodavirus RNA2 segment to DNA probe that is tethered surface screen-printed In proof-of-principle test, synthetic sequence detected based on competitive hybridization between two oligonucleotides (biotinylated reporter target RNA)...
The reported study was carried out from October 2014 to January 2015 and involved 622 cattle belonging a dairy farm in North Tunisia. Each animal examined once for the presence of lice. infestation prevalence estimated at 14.3% (89/622). Three louse species were collected: Linognathus vituli (2.7%), Haematopinus eurysternus (11.4%) Bovicola bovis (0.8%). co-infestation 0.6% concerned only B. L. vituli, collected four calves (three males one female). Calves infested by three species, (17.5%),...
Spondylocostal dysostosis is a rare genetic disorder caused by mutations in DLL3, MESP2, LFNG, HES7, TBX6, and RIPPLY2. A particular form of this characterized the association spondylocostal with multiple pterygia has been reported called spondylospinal thoracic dysostosis. Both disorders affect spine ribs, leading to abnormal development spine. Spondylospinal syndrome vertebral segmentation defects, cage deformity, pterygia. This can be considered different described However, no testing...