A5015296826- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Xenotransplantation and immune response
- Renal and related cancers
- Renal cell carcinoma treatment
- Genomics and Phylogenetic Studies
- Immune cells in cancer
- Virus-based gene therapy research
- Cancer Genomics and Diagnostics
- Bioactive Compounds and Antitumor Agents
- Nanoplatforms for cancer theranostics
- Cancer, Stress, Anesthesia, and Immune Response
- Microbial Community Ecology and Physiology
- Prostate Cancer Treatment and Research
- Epigenetics and DNA Methylation
- Photodynamic Therapy Research Studies
- MicroRNA in disease regulation
- Immune Response and Inflammation
- Glycosylation and Glycoproteins Research
LMU Klinikum
2019-2023
Ludwig-Maximilians-Universität München
2010-2023
Helmholtz Zentrum München
2009-2017
Center for Environmental Health
2009
Klinikum rechts der Isar
2007
Institute for Environment and Human Security
2003-2007
Max Delbrück Center
2007
Charité - Universitätsmedizin Berlin
2007
Technical University of Munich
1985-2005
Munich Security Conference
2005
FEHRENBACH, E., F. PASSEK, A. M. NIESS, H. POHLA, C. WEINSTOCK, H.-H. DICKHUTH, and NORTHOFF. HSP expression in human leukocytes is modulated by endurance exercise. Med. Sci. Sports Exerc., Vol. 32, No. 3, pp. 592–600, 2000. Purpose: Temperature increase, oxidative stress, inflammatory reactions after exercise were expected to stimulate the synthesis of heat shock proteins (HSP) peripheral blood leukocytes. Furthermore, it was interest whether regular training influences expression. Methods:...
In many solid tumors, cancer stem cells (CSC) represent a population with tumor-initiating, self-renewal, and differentiation potential, which can be identified by surface protein markers. No generally applicable markers are yet known for renal cell carcinoma (RCC). Two RCC lines (RCC-26, RCC-53) were found to differ widely in their capacity form spheres vitro establish tumors mice, potentially reflecting differences CSC content. A subpopulation expressing the CXC chemokine receptor 4...
Abstract Purpose: Renal cell carcinoma harbors high numbers of infiltrating lymphocytes with apparent limited efficacy in tumor control. This study focused on the natural killer (NK) cells renal carcinoma. Experimental Design: Tumor-infiltrating (TIL) were isolated from and analyzed for NK frequency phenotype (n = 34). enriched tested effector function. Results: Two subtypes identified, one containing (>20% lymphocyte population, n 14), other low (<20%, 20), numbers. both...
Abstract Background For optimal T cell activation it is desirable that dendritic cells (DCs) display peptides within MHC molecules as signal 1, costimulatory 2 and, in addition, produce IL-12p70 3. polarizes responses towards CD4 + helper 1 cells, which then support the development of CD8 cytotoxic lymphocytes. We therefore developed new maturation cocktails allowing DCs to biologically active for large-scale cancer vaccine development. Methods After elutriation leukapheresis products a...
Abstract BACKGROUND Prostate cancer is the most common of men in Western world. Despite over‐expression tumor‐associated antigens, like PSA or PSMA, immune activation inefficient. The goal this investigation was to assess situ characteristics prostate cancer‐infiltrating lymphocytes and determine their status effector function. METHODS We compared 17 carcinoma containing tissues, four benign prostatic hyperplasia tissues eight healthy regarding lymphocyte subset composition, locoregional...
Abstract Among tumor‐infiltrating lymphocytes (TILs) directly isolated from renal cell carcinomas (RCCs), we found substantial numbers of natural killer (NK) cells in most tumor tissues. They could be identified reliably situ with an antibody directed against the activating receptor (AR) NKp46 that is exclusively expressed by all NK cells. NK‐enriched TILs (NK‐TILs) showed cytotoxicity major histocompatibility complex (MHC) class I‐negative lines. The ability to detect lysis target was...
Bispecific monoclonal antibodies (bsAbs) are a promising immunotherapeutic option for treatment of cancer, especially in situations minimal residual disease. The combination an anti-CD3 and anti-tumor-associated antigen antibody redirects cytotoxic T-lymphocytes towards malignant cells. Using trifunctional bispecific against Ep-CAM × CD3, that additionally activates FcγR + accessory cells via its Fc region, we investigated the interaction between three EpCAM prostate carcinoma cell lines...
Four similar strains of gram-negative, red or pink, radiation-resistant, rod-shaped bacteria were isolated from animal feces and freshwater fish. Cell division was a simple type. The deoxyribonucleic acid guanineplus-cytosine (G + C) base ratio 69 mol%, menaquinone (MK-8) found in their respiratory chain. predominant cellular fatty acids C15:1 C16:1. Ornithine the diamino peptidoglycan, peptidoglycan type representative strain ornithine-glycine2 (A3β) They stained gram negative (by Hucker...
No AccessJournal of UrologyInvestigative Urology1 Feb 2014Expression and Prognostic Significance a Comprehensive Epithelial-Mesenchymal Transition Gene Set in Renal Cell Carcinoma Dong Chen, Maximilian Gassenmaier, Matthias Maruschke, Rainer Riesenberg, Heike Pohla, Christian G. Stief, Wolfgang Zimmermann, Alexander Buchner ChenDong Chen Department Urology, University Hospital, Munich, Germany Zhongnan Hospital Wuhan University, Wuhan, People's Republic China , GassenmaierMaximilian...
We improved tumor cell detection compared with currently available immunocytochemical methods by immunomagnetic enrichment.Two of enrichment using antibody coated magnetic beads were tested and unenriched immunocytochemistry, including positive selection epithelial cells the antiepithelial BER-EP4 depletion mononuclear anti-leukocyte CD45. Various numbers from 4 tissue culture lines DU 145, RT-4, KTCTL-2 KTCTL-30 obtained urological tumors added to whole blood isolated density...
Properdin plays an important regulatory role in the activation of complement system. Here we report biosynthesis properdin four different human T cell lines and cells purified from peripheral blood. Cell sorting experiments, conjunction with Northern blotting, showed that both CD4- CD8-bearing populations have potential to synthesize properdin. The functional activity secreted these was determined a hemolytic assay. In view numerous ways which may influence cellular immune response, present...
No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Feb 2003Isolation Circulating Cancer Cells From Whole Blood By Immunomagnetic Cell Enrichment and Unenriched Immunocytochemistry In Vitro RICHARD E. ZIGEUNER, RAINER RIESENBERG, HEIKE POHLA, ALFONS HOFSTETTER, RALPH OBERNEDER ZIGEUNERRICHARD ZIGEUNER , RIESENBERGRAINER RIESENBERG POHLAHEIKE POHLA HOFSTETTERALFONS HOFSTETTER OBERNEDERRALPH View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)63996-1AboutFull TextPDF ToolsAdd to...
Abstract Background As a step towards clinical cardiac xenotransplantation, our experimental heterotopic intrathoracic xenotransplantation model offers beating and ejecting donor heart while retaining the recipient′s native organ as backup in case of graft failure. Clinically applicable immunosuppressive regimens ( IS ) were investigated first, then treatments known to be effective hypersensitized patients or those with recalcitrant rejection reactions. Methods Consecutive experiments...
The aim of the study was to develop a new therapeutic strategy target cancer stem cells (CSCs) in clear cell renal carcinoma (ccRCC) and identify typical CSC markers improve therapy effectiveness. It found that corrected-mRNA expression-based stemness index upregulated kidney (KIRC) tissues compared non-tumor tissue increased with higher tumor stage grade. EZH2 identified as marker prognosis factor for KIRC patients. expression associated several activated tumor-infiltrating immune cells....
Abstract Background Cancer stem cells (CSCs) are a small subpopulation of tumor with the capability self‐renewal and drug resistance, leading to progression disease relapse. Our study aimed investigate antitumor effect berbamine, extracted from berberis amurensis, on prostate CSCs. Methods Sphere formation was used collect The viability, proliferation, invasion, migration, apoptosis assays were evaluate berbamine Prostate CSC markers analyzed by flow cytometry qRT‐PCR. Small RNA sequencing...