A5013265768- Advanced biosensing and bioanalysis techniques
- NF-κB Signaling Pathways
- Cancer-related molecular mechanisms research
- Biosensors and Analytical Detection
- Analytical Chemistry and Sensors
- Cytokine Signaling Pathways and Interactions
- Advanced Biosensing Techniques and Applications
- Molecular Biology Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Mass Spectrometry Techniques and Applications
- Gene expression and cancer classification
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Electrochemical Analysis and Applications
- Electrochemical sensors and biosensors
- Immune Response and Inflammation
- Single-cell and spatial transcriptomics
- Lung Cancer Treatments and Mutations
- Colorectal Cancer Treatments and Studies
- Computational Drug Discovery Methods
- Air Quality and Health Impacts
- Integrated Circuits and Semiconductor Failure Analysis
- Energy Harvesting in Wireless Networks
- Advanced Memory and Neural Computing
- Semiconductor materials and devices
Imperial College London
2016-2025
NIHR Imperial Biomedical Research Centre
2017-2021
Inspire
2020
University of Patras
2014-2018
General University Hospital of Patras
2015-2016
<italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">ESR1</i> mutations are important biomarkers in metastatic breast cancer. Specifically, p.E380Q and p.Y537S arise response to hormonal therapies given patients with hormone receptor positive (HR+) cancer (BC). This paper demonstrates the efficacy of an ISFET based CMOS integrated Lab-on-Chip (LoC) system, coupled variant-specific isothermal amplification chemistries, for detection discrimination...
Abstract Breast cancer (BC) is a common in women worldwide. Despite advances treatment, up to 30% of eventually relapse and die metastatic breast cancer. Liquid biopsy analysis circulating cell-free DNA fragments the patients’ blood can monitor clonality evolving mutations as surrogate for tumour biopsy. Next generation sequencing platforms digital droplet PCR be used profile from liquid biopsies; however, they are expensive time consuming clinical use. Here, we report novel strategy with...
Prostate cancer (PCa) is a highly prevalent disease, causing the second largest amount of male deaths worldwide. Currently, prostate specific antigen (PSA) blood test remains standard serum prognostic and diagnostic monitoring biomarker but it lacks specificity sensitivity. PSA testing can lead to invasive biopsies which result in under detection clinically significant disease potential overtreatment indolent disease. There are promising circulating biomarkers could facilitate less more...
Abstract Cervical cancer affects over half a million people worldwide each year, the majority of whom are in resource-limited settings where cytology screening is not available. As persistent human papilloma virus (HPV) infections key causative factor, detection HPV strains now complements services exist. This work demonstrates efficacy handheld Lab-on-Chip (LoC) device, with an external sample extraction process, detecting cervical from biopsy samples. The device based on Ion-Sensitive...
This paper presents a low power current-mode method for monitoring differentially derived changes in pH from ion-sensitive field-effect transistor (ISFET) sensors, by adopting the Chemical Gilbert Cell. The fabricated system, with only few transistors, achieves differential measurements and therefore drift minimisation of continuously recorded signals obtained biochemical reactions such as DNA amplification addition to combined gain tunability using single current. Experimental results are...
This paper gives an overview of how CMOS design methods can be applied to ion-sensitive field effect transistor (ISFETs) for pH-based DNA methylation and miRNA detection. Design specifications are fundamentally defined by the choice analysis. As such, focus was on developing front-end analogue circuits carry out Methylation-specific PCR (MSP) Point-of-Care applications, sequencing detailed The use MSP prompted ISFET weak inversion current mirror topology differential sensing reduction drift...
Prostate cancer (PCa) is the second most common cause of male cancer-related death worldwide. The gold standard treatment for advanced PCa androgen deprivation therapy (ADT). However, eventual failure ADT and leads to lethal metastatic castration-resistant PCa. As such, detection relevant biomarkers in blood drug resistance patients could lead personalized options. mRNA often limited by low specificity qPCR assays which are restricted specialized laboratories. Here, we present a novel...
This paper presents the concept of a ratiometric approach for DNA methylation detection using "Methylation Cell" indication epigenetic abnormalities related to cancer. The allows real-time pH signals resulting from based reactions ISFET sensors, performing continuous computation ratio methylation, giving discrete output signal when exceeds certain predefined percentage. Fabricated in typical 0.35μm CMOS process, it uses current-mode translinear circuits perform low power. Experimental...
A growing number of studies has shed light on the role NF-κΒ in non-small-cell lung cancer (NSCLC). To address significance major effectors alternative pathway, we investigated relationship between NF-κΒ2, RelB, NIK and Bcl3 expression (mRNA protein) clinical outcome NSCLC patients. protein levels were assessed by immunohistochemistry tissue samples from 151 patients who had curative resection. mRNA also evaluated 69 using quantitative real-time PCR. Although all studied proteins...
Ion-sensitive field-effect transistors (ISFETs) in combination with unmodified complementary metal oxide semiconductors present a point-of-care platform for clinical diagnostics and prognostics. This work illustrates the sensitive specific detection of two circulating mRNA markers prostate cancer, androgen receptor TMPRSS2-ERG fusion using target-specific loop-mediated isothermal amplification method. RNA were detected down to 3x10 <inline-formula...
We demonstrate a diagnostics platform which integrates an ISFET array and temperature control loop for isothermal DNA detection. The controller maintains of 63°C to perform nucleic acid amplification is detected by the on-chip sensors. 32×32 first calibrated cancel trapped charge then measures change in pH reaction. sensor data sent microcontroller reaction monitored real-time using MATLAB interface. Experiments confirm 0.9 when tested presence RNA associated with Zika virus.
Abstract During the last decade, a growing number of publications implicate NF-kB2 in NSCLC pathogenesis. Here, we investigated clinical relevance single nucleotide polymorphisms (SNPs) rs7897947, rs11574852 and rs12769316 their association with protein mRNA levels. Our data show that TT (rs7897947T >G ) AA (rs12769316G >A) genotypes were strongly associated an increased risk for (P = 0.019 P 0.003, respectively). Additionally, multivariate analysis, homozygosity was worse 2- 3-year...
A trapped charge compensation scheme for ISFET based translinear circuits is presented, as part of a system prediction cancer risk, on DNA methylation. Each pixel able to measure methylation ratio through pH-based measurements and by using in-pixel comparison tunable threshold, output result which indicates percentage used score. The developed was designed in 0.35 μm CMOS technology uses novel ISFETs circuits. compensate up 380mV, with error below 5%, range ratios between 50% 80% generated...
An increasing number of studies implicates the NF-κB (Nuclear Factor kappa light chain gene enhancer in B cells) alternative pathway non-small-cell lung cancer (NSCLC). We assessed clinical significance CD40 (Tumor necrosis factor receptor superfamily member 5, TNFRSF5), BAFFR (B-cell activating receptor), RANK (Receptor activator NF-κB) and LTβR (lymphotoxin β receptor) receptors, which activate NF-κB, NSCLC. Evaluation CD40, BAFFR, expression was performed based on Cancer Genome Atlas...
The COVID-19 pandemic has highlighted a significant research gap in the field of molecular diagnostics. This brought forth need for AI-based edge solutions that can provide quick diagnostic results whilst maintaining data privacy, security and high standards sensitivity specificity. paper presents novel proof-of-concept method to detect nucleic acid amplification using ISFET sensors deep learning. enables detection DNA RNA on low-cost portable lab-on-chip platform identifying infectious...
Ion-sensitive field-effect transistors (ISFETs) in combination with unmodified complementary metal oxide semiconductors present a point-of-care platform for clinical diagnostics and prognostics. This work illustrates the sensitive specific detection of two circulating mRNA markers prostate cancer, androgen receptor TMPRSS2-ERG fusion using target-specific loop-mediated isothermal amplification method. RNA were detected down to 3x10
Expression of Transcribed Ultraconserved Regions (T-UCRs) is often deregulated in cancer. The present study assesses the expression and methylation three T-UCRs (Uc160, Uc283 Uc346) colorectal cancer (CRC) explores potential T-UCR circulating DNA for detection adenomas adenocarcinomas. levels Uc160, Uc346 were lower neoplastic tissues from 64 CRC patients (statistically significant p<0.001), compared to non-malignant tissues, while displayed inverse pattern (p<0.001, p=0.001 p=0.004...
This paper presents a method for detection of ESR1 p.E380Q, common Breast Cancer (BC) mutation, using an ISFET (Ion-Sensitive Field-Effect Transistor) based Lab-on-Chip (LoC) platform. The LoC contains array that can detect pH changes during DNA amplification, specifically Loop-Mediated Isothermal Amplification (LAMP). Synthetic was detected in comparison pH-LAMP assay, carried out on the platform as well conventional qPCR instrument. Positive allele arises due to bespoke allele-specific...