A5077882715- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Hepatitis Viruses Studies and Epidemiology
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- Viral Infections and Vectors
- Influenza Virus Research Studies
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Child Nutrition and Water Access
- Immunodeficiency and Autoimmune Disorders
- Systemic Lupus Erythematosus Research
- Animal Disease Management and Epidemiology
- SARS-CoV-2 detection and testing
- T-cell and Retrovirus Studies
- Virology and Viral Diseases
- Monoclonal and Polyclonal Antibodies Research
- Rabies epidemiology and control
- Cytomegalovirus and herpesvirus research
- Vector-Borne Animal Diseases
- Viral Infections and Outbreaks Research
- Malaria Research and Control
- HIV Research and Treatment
- Yersinia bacterium, plague, ectoparasites research
Instituto Adolfo Lutz
2015-2024
Secretaria da Saúde
2010-2022
Universidade de São Paulo
2011-2013
Public Health Dayton & Montgomery County
2010
Ministério da Saúde
2010
Instituto Pasteur
2009
National Institutes of Health
1997
National Institute of Allergy and Infectious Diseases
1997
United States Food and Drug Administration
1994
A new seminested PCR typing assay has been extended to identify the important veterinary rotavirus serotypes G5, G6, G10, and G11, as well rare human serotype G8. The specificity of method was evaluated with 30 standard laboratory strains G1 G6 G8 G11 types. Rotavirus strain types G8, not previously recognized in pigs, were identified field specimens porcine origin.
A PCR typing assay was developed to identify rotavirus P types (VP4 specificity) of bovine NCDV, UK, and B223 strains porcine OSU Gottfried strains. Thirty-nine human animal representing all known, some undefined, were used develop evaluate the specificity method. No cross-amplification observed. The results agreed with previous characterizations by monoclonal antibodies, sequence analysis, hybridization assays, except for strain, which showed a type distinct from asymptomatic Analysis small...
We conducted the genome sequencing and analysis of first confirmed COVID-19 infections in Brazil. Rapid coupled with phylogenetic analyses context travel history corroborate multiple independent importations from Italy local spread during initial stage transmission
Rotavirus strains causing gastroenteritis in Brazilian children were characterized by PCR-based typing assays. In addition to bearing the major human G and P types, large numbers of P3 (M37-like), P6 (HCR3-like), untypeable complex mixtures types not previously recognized present community.
Background Despite the WHO recommendation that 2010–2011 trivalent seasonal flu vaccine must contain A/California/7/2009/H1N1-like virus there is no consistent data regarding its immunogenicity and safety in a large autoimmune rheumatic disease (ARD) population. Methods 1668 ARD patients (systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), ankylosing spondylitis (AS), systemic sclerosis, psoriatic (PsA), Behçet's (BD), mixed connective tissue disease, primary antiphospholipid...
Objective. To assess the immunogenicity and safety of non-adjuvanted influenza A H1N1/2009 vaccine in patients with juvenile autoimmune rheumatic disease (ARD) healthy controls, because data are limited to adult rheumatologic population. Methods. total 237 ARD [juvenile systemic lupus erythematosus (JSLE), idiopathic arthritis (JIA), dermatomyositis (JDM), scleroderma, vasculitis] 91 controls were vaccinated. Serology for anti-H1N1 was performed by hemagglutination inhibition assay....
In 2013, the equine-like G3P[8] DS-1-like rotavirus (RVA) strain emerged worldwide. 2016, this was reported in northern Brazil. The aims of study were to conduct a retrospective genetic investigation identify possible entry these atypical strains Brazil and describe their distribution across representative area country. From 2013 2017, total 4226 faecal samples screened for RVA by ELISA, PAGE, RT-PCR sequencing. represented 20.9 % (167/800) all RVA-positive samples, further subdivided as...
São Paulo, a densely inhabited state in southeast Brazil that contains the fourth most populated city world, recently experienced its largest yellow fever virus (YFV) outbreak decades. YFV does not normally circulate extensively so people were unvaccinated when began. Surveillance non-human primates (NHPs) is important for determining magnitude and geographic extent of an epizootic, thereby helping to evaluate risk spillover humans. Data from infected NHPs can give more accurate insights...
Abstract Yellow Fever (YF) is a severe disease caused by Virus (YFV), endemic in some parts of Africa and America. In Brazil, YFV maintained sylvatic transmission cycle involving non-human primates (NHP) forest canopy-dwelling mosquitoes, mainly Haemagogus -spp Sabethes- spp. Beginning 2016, Brazil faced one the largest outbreaks recent decades, southeastern region. São Paulo city, was detected October 2017 Aloutta monkeys an Atlantic Forest area. From 542 NHP, total 162 NHP were positive...
Dengue Fever and Hemorrhagic are diseases affecting approximately 100 million people/year a major concern in developing countries. In the present study, phylogenetic relationship of six strains first autochthonous cases DENV-4 infection occurred Sao Paulo State, Parana State Rio Grande do Sul Brazil, 2011 were studied. Nucleotide sequences envelope gene determined compared with representative genotypes I, II, III Sylvatic for DEN4 retrieved from GenBank. We employed Bayesian approach to...
Background Immunosuppressed individuals present serious morbidity and mortality from influenza, therefore it is important to understand the safety immunogenicity of influenza vaccination among them. Methods This multicenter cohort study evaluated reactogenicity an inactivated, monovalent, non-adjuvanted pandemic (H1N1) 2009 vaccine elderly, HIV-infected, rheumatoid arthritis (RA), cancer, kidney transplant, juvenile idiopathic (JIA) patients. Participants were included during routine...
Regarding public health in Brazil, a new scenario emerged with the establishment of universal rotavirus (RV) vaccination programs. Herein, data from five years surveillance (2007-2012) G- and P-type RV strains isolated individuals acute gastroenteritis Brazil are reported. A total 6,196 fecal specimens were investigated by ELISA RT-PCR. RVs detected 19.1% (1,181/6,196). The peak incidence moved June-August to September. was less frequently (19.5%) among children ≤ 5 than older adolescents...
Abstract A total of 3,101 fecal specimens were collected during an 8-year survey for rotavirus infection in São Paulo, Brazil. Group was detected 774 (25.0%) specimens. Of these, 431 strains (55.7%) analyzed G and P types by reverse transcription–polymerase chain reaction; G1 the predominant serotype (68.2%), followed G9 (17.2%), G4 (6.3%), G2 (1.2%), G3 (0.7%), mixed (1.8%), untypeable (4.6%). Both could be established 332 (77.0%). We identified 4 most common worldwide: P[8]G1 (66.6%),...
Despite WHO recommendations about the A/California/7/2009/H1N1-like virus vaccination, studies evaluating its possible influence on clinical manifestations and autoantibody profile in primary Sjögren's syndrome (SS) are scarce. The aim of this study was to evaluate unadjuvanted vaccination SS short/long-term. Thirty-six patients (The American-European Consensus Group Criteria, 2002) 36 healthy controls with comparable mean age gender were evaluated before 21-days after regarding...
Rotavirus serotype G5 isolates were recently recovered from children with diarrhoea in Brazil. Like most human strains, they exhibited long electro- pherotypes and subgroup II Wa-like VP4 specificity. We report the successful propagation molecular antigenic characterization of one these (IAL-28). Cross-neutralization IAL-28 a single gene reassortant, UK × IAL- 28, which contains encoding VP7 genomic background, prototype G1 to G14 rotaviruses demonstrated that has determinants specific for...
Hand, foot and mouth disease (HFMD) is a contagious enteroviral infection occurring primarily in children characterized by vesicular palmoplantar eruptions erosive stomatitis. Echovirus 4 (EV-4) has been commonly associated with aseptic meningitis. The association of HFMD EV-4 not reported previously. Two samples 14-month child who presented mild fever, sores the mouth, rash blisters on palm hands soles feet were sent to Enteric Viruses Laboratory Adolfo Lutz Institute. Clinical inoculated...
ABSTRACT Previously, we reported the development of a microarray-based method for identification five clinically relevant G genotypes (G1 to G4 and G9) (V. Chizhikov et al., J. Clin. Microbiol. 40:2398-2407, 2002). The expanded version rotavirus microarray assay presented herein is capable identifying (i) human VP4 (P[4], P[6], P[8], P[9], P[14]) (ii) additional VP7 (G5, G6, G8, G10, G12) on one chip. Initially, total 80 cell culture-adapted animal reference strains known P (P[1] P[12],...