A5055368601- Ubiquitin and proteasome pathways
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Sirtuins and Resveratrol in Medicine
- Cell death mechanisms and regulation
- Peptidase Inhibition and Analysis
- PARP inhibition in cancer therapy
- Cancer-related Molecular Pathways
- Adipose Tissue and Metabolism
- Protease and Inhibitor Mechanisms
- Signaling Pathways in Disease
- Cancer, Hypoxia, and Metabolism
- Calcium signaling and nucleotide metabolism
- Chromatin Remodeling and Cancer
- Microbial Metabolism and Applications
- DNA Repair Mechanisms
- Chronic Kidney Disease and Diabetes
- Genetics and Neurodevelopmental Disorders
- Nuclear Receptors and Signaling
- Hedgehog Signaling Pathway Studies
- Mechanisms of cancer metastasis
- Metabolism and Genetic Disorders
- Glutathione Transferases and Polymorphisms
- Atherosclerosis and Cardiovascular Diseases
- Developmental Biology and Gene Regulation
First Hospital of China Medical University
2020-2024
China Medical University
2019-2024
The sarcomeric interaction of α-myosin heavy chain (α-MHC) with Titin is vital for cardiac structure and contraction. However, the mechanism regulating this in normal failing hearts remains unknown. Lactate a crucial energy substrate heart. Here, we identify that α-MHC undergoes lactylation on lysine 1897 to regulate Titin. We observed reduction K1897 mice patients heart failure. Loss K1897R knock-in reduces α-MHC-Titin leads impaired function. Furthermore, identified p300 Sirtuin 1 act as...
Hypertension can lead to podocyte damage and subsequent apoptosis, eventually resulting in glomerulosclerosis. Although alleviating apoptosis has clinical significance for the treatment of hypertensive nephropathy, an effective therapeutic target not yet been identified. The function septin4, a proapoptotic protein important marker organ damage, is regulated by post-translational modification. However, exact role septin4 regulating its connection renal remains unclear. We investigated...
Oxidative stress-associated endothelial injury is the initial event and major cause of multiple cardiovascular diseases such as atherosclerosis hypertensive angiopathy. A protein homeostasis imbalance a critical injury, homologous to E6AP C-terminus (HECT)-type E3 ubiquitin ligases are core factors controlling homeostasis. Although HECT-type involved in regulation cardiac development diseases, their roles remain largely unknown. This study aimed identify which ligase clarify mechanisms at...
Poly(ADP-ribose) polymerase 1 (PARP1) has a major regulatory role in cardiovascular disease. However, inhibiting PARP1 activity does not significantly improve clinical outcomes of disease, which suggests that the mechanism disease is unclear. Here, we focused on deacetylation mechanisms and crosstalk post-translational modifications. We uncovered crucial molecular interactions protein modifications deacetylase Sirtuin 2 (SIRT2) vascular damage. The results showed SIRT2 was involved this...
Oxidative stress-associated endothelial damage is the initiation factor of cardiovascular disease, and protein posttranslational modifications play critical roles in this process. Bcl-2-associated athanogene 3 (BAG3) a molecular chaperone regulator BAG family, which interacts with various proteins influences cell survival by activating multiple pathways. BAG3 undergoes modifications; however, research evaluating acetylation its regulatory mechanism lacking. In addition, interacting oxidative...
Abstract Background Endothelial injury caused by Type 2 diabetes mellitus (T2DM) is considered as a mainstay in the pathophysiology of diabetic vascular complications (DVCs). However, molecular mechanism T2DM-induced endothelial remains largely unknown. Here, we found that WW domain-containing E3 ubiquitin protein ligase (WWP2) act novel regulator for through modulating ubiquitination and degradation DEAD-box helicase 3 X-linked (DDX3X). Methods Single-cell transcriptome analysis was used to...
SIRT2-mediated deacetylation of SMC1A promotes its phosphorylation and overcomes the oncogenic stress for tumor cell survival.
Abstract WWP2 is a HECT‐type E3 ubiquitin ligase that regulates various physiological and pathological activities by binding to different substrates, but its function regulatory mechanism in vascular smooth muscle cells (VSMCs) are still unknown. Here, we clarified the role of regulation SIRT1‐STAT3 impact this process VSMCs. We demonstrated expression was significantly increased angiotensin II‐induced VSMCs model. Knockdown inhibited proliferation, migration phenotypic transformation,...
Abstract Oxidative stress injury is involved in many cardiovascular diseases, like hypertension and myocardial infarction. Ubiquitination a ubiquitous protein post‐translational modification that controls wide range of biological functions plays crucial role maintaining the homeostasis cells physiology disease. Many studies have shown oxidative damage inextricably linked to ubiquitination. We demonstrate Smurf2, an E3 ubiquitinated ligase, HUVEC apoptosis induced by alleviate H 2 O ‐induced...
SIRT1 and STAT3 are key to human aortic vascular smooth muscle cells (HAVSMCs) proliferation, migration phenotypic transformation, but the regulatory mechanism of SIRT1-STAT3 in this process is still unclear.Septin4 a cytoskeleton-related protein that regulates oxidative stress-vascular endothelial injury.However, role underlying Septin4 atherosclerosis remains unknown.Here, we revealed regulating atherosclerosis.We determined expression were markedly increased Apoe -/-atherosclerosis mice...
Abstract Acute lymphoblastic leukemia (ALL) has been a huge threat for people's health and finding effective target therapy is urgent important. WWP2, as one of E3 ubiquitin ligase, involved in many biological processes by specifically binding to substrates. PARP1 plays role cell apoptosis considered therapeutic certain cancers. In this study, we firstly found that WWP2 expressed higher newly diagnosed ALL patients comparing with complete remission (CR) normal control people, relapse than...
Oxidative stress participates in several heart diseases and is an important mechanism contributing to the pathological alterations of myocardial cell injury. In recent years, ubiquitylation has been demonstrated be biochemical reaction associated with apoptosis. To investigate effects interactions E3 ligase F‑box WD repeat domain containing 7 (Fbw7) MCL1 apoptosis regulator, BCL2 family member (Mcl‑1) cells during oxidative stress, Cell Counting Kit‑8, flow cytometry, western blot, reactive...
Hypertensive nephropathy (HN), mainly caused by chronic hypertension, is one of the major causes end-stage renal disease. However, pathogenesis HN remains unclarified, and there an urgent need for improved treatments. Gene expression profiles normal tissue were obtained from Expression Omnibus database. A total 229 differentially co-expressed genes identified weighted gene co-expression network analysis differential analysis. These used to construct protein–protein interaction networks...
Abstract Septin4, a protein localized at mitochondrion, can promote cells apoptosis mainly by binding XIAP (X-linked inhibitors of apoptosis), however, nothing is known about the role and mechanism Septin4 in cardiomyocytes apoptosis. Here current study, we report that HIF-1α (hypoxia-inducible factor 1 alpha) novel interacting with Septin4-GTPase domain. In addition, enhances between E3 ubiquitin ligase VHL (von Hippel-Lindau protein) to down-regulate HIF-1α, reducing cardio-protective...
As a widely used lipid-lowering drug in clinical practice, atorvastatin is recognized for its role protecting vascular endothelium the cardiovascular system. However, clear mechanistic understanding of action lacking. Here, we found that counteracted angiotensin II-induced endothelial injury mice with hypertension. Mechanistically, up-regulated WWP2, E6AP C-terminus (HECT)-type E3 ubiquitin ligase an essential regulating protein ubiquitination and various biological processes, thereby...
Abstract Backgrounds: Myocardial ischemia, characterized by insufficient nutrients and oxygen supply, is the most common cause of ischemic heart disease. Hypoxia-induced cardiomyocytes injury pathogenic feature myocardial ischemia. Although previous studies have reported that proapoptotic protein Septin4 contributes to prevent some cancers promoting tumor cells apoptosis mainly through X-linked inhibitors (XIAPs), little known its role in hypoxia-induced other new interacting partner....
DNA polymerase gamma (PolG) is the major of mitochondrial (mtDNA) and essential for stabilizing function. Vascular calcification (VC) common senescence related degenerative pathology phenomenon in end-stage multiple chronic diseases. Mitochondrial dysfunction was often observed calcified vessels, but function mechanism PolG process still unknown. The present study found PolGD257A/D257A mice presented more severe aortas than wild type (WT) with vitamin D3 (Vit D3) treatment, this also...