A5051198131- DNA and Nucleic Acid Chemistry
- Protein Structure and Dynamics
- Nanopore and Nanochannel Transport Studies
- Electrostatics and Colloid Interactions
- Bacterial Genetics and Biotechnology
- Microfluidic and Capillary Electrophoresis Applications
- Digital Platforms and Economics
- Bacteriophages and microbial interactions
- Supply Chain and Inventory Management
- Advanced NMR Techniques and Applications
- Sustainable Supply Chain Management
- Nonlinear Dynamics and Pattern Formation
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Consumer Market Behavior and Pricing
- Complex Systems and Time Series Analysis
- Liver Disease Diagnosis and Treatment
- Transportation and Mobility Innovations
- Spectroscopy and Quantum Chemical Studies
- Central Venous Catheters and Hemodialysis
- Scheduling and Optimization Algorithms
- Effects and risks of endocrine disrupting chemicals
- Chemistry and Chemical Engineering
- Enzyme Structure and Function
- Chaos control and synchronization
Beijing Chaoyang Emergency Medical Center
2025
Dongguan University of Technology
2020-2024
Southern Medical University
2024
Nanfang Hospital
2024
Sun Yat-sen University
2020-2022
The University of Texas Medical Branch at Galveston
2015-2022
Shenzhen Sixth People's Hospital
2020
South China University of Technology
2015-2019
Jiangsu Cancer Hospital
2017
Nanjing Medical University
2017
Abstract Alzheimer’s disease (AD) is the most common cause of dementia, and its underlying mechanisms have been a subject great interest. The mainstream theory AD pathology suggests that primarily associated with tau protein amyloid-beta (Aβ). However, an increasing body research has revealed abnormalities in lipid metabolism may be important event throughout pathophysiology AD. Astrocytes, as members network brain, play significant role this event. study abnormal astrocytes provides new...
Basic side chains play major roles in recognition of nucleic acids by proteins. However, dynamic properties these positively charged are not well understood. In this work, we studied changes conformational dynamics basic upon protein–DNA association for the zinc-finger protein Egr-1. By nuclear magnetic resonance (NMR) spectroscopy, characterized all side-chain cationic groups free and complex with target DNA. Our NMR order parameters indicate that arginine guanidino interacting DNA bases...
Renowned luxury brand Bvlgari has made a name for itself on the international scene because to its superb craftsmanship and distinctive fusion of traditional modern design. This study explores Bvlgari's product offers, price, distribution methods, promotional activities as it applies 4P analysis marketing strategy. A SWOT also highlights disadvantages, such small market share past PR issues, in addition advantages, which include unique design philosophy captivating narrative. Threats growth...
Background Intracavitary electrocardiogram (IC ECG) guidance emerges as a new technique for peripherally inserted central catheters (PICCs) placement and demonstrates many potential advantages in recent observational studies. Aims To determine whether IC ECG-guided PICCs provide more accurate positioning of catheter tips compared to conventional anatomical landmarks patients with cancer undergoing chemotherapy. Methods In this multicenter, open-label, randomized controlled study...
Intermolecular ion pairs (salt bridges) are crucial for protein-DNA association. For two complexes, we demonstrate that the of protein side-chain NH3+ and DNA phosphate groups undergo dynamic transitions between distinct states in which charged moieties either direct contact or separated by water. While crystal structures complexes show only solvent-separated pair (SIP) state some interfacial lysine side chains, our NMR hydrogen-bond scalar coupling data clearly indicate presence (CIP) same...
Detailed modeling of the free solution electrophoresis five proteins (bovine α-lactalbumin, hen egg white lysozyme, bovine superoxide dismutase, human carbonic anhydrase II, and ovalbumin) is carried out within framework continuum primitive model. Protein crystal structures translational diffusion constants are used to design parametrize models. The results compared with experimental mobilities protein charge ladders, collections derivatives where number groups varied by partial acylation...
Understanding interactions between DNA molecules in water the presence of ions is an important issue biological physics. The authors use molecular dynamics simulations to explain how free-energy landscape DNA-DNA depends on separation and orientation molecules, provide insights into mechanisms behind specific double-stranded solution.
In this work, the components of protein electrostatic potentials in solution are analyzed with NMR paramagnetic relaxation enhancement experiments and compared continuum theory, multiscale simulations. To determine contributions components, we analyze them at different ionic strengths from 0 to 745 mM. A theoretical approximation allows determination potential a given proton without reference structure ratio enhancements rates between cationic an anionic probe. The results derived...
The electrophoretic mobility of short 18 and 20 bp duplex DNAs is modeled by an iterative boundary element procedure that numerically solves the coupled Poisson, low Reynolds Number Navier−Stokes, ion transport equations. Both capped cylinder (CC) "detailed" models derived from secondary structure fragments are examined. Translation−rotation coupling examined with regard to detailed models, it concluded this has very little effect on either diffusion or electrophoresis. When buffer consists...
Abstract The monomer and dimer of the bacterium Serratia marcescens endonuclease (SMnase) are each catalytically active two subunits function independently other. Specific interfacial waters may play a role in stability, complex formation, functionality. We performed molecular dynamics simulations both subunit SMnase its model built with DNA analyzed relation hydration sites to catalytic mechanism. It was found that binding has little influence on global properties protein, including...
The monomer and dimer of the bacterium Serratia marcescens endonuclease (SMnase) are each catalytically active, two subunits function independently other. Nature, however, chooses form instead monomer. In order to explain this, we performed molecular dynamics (MD) simulations both model-built complexes a subunit SMnase with DNA in aqueous solution. We estimated electrostatic binding energy, analyzed distribution water around complexes, identified clusters protein, related protein's function....
Due to chemical exchange, the mobility of histidine (His) side chains proteins is typically difficult analyze by NMR spectroscopy. Using an approach that uninfluenced we investigated internal motions His imidazole NH groups directly interact with DNA phosphates in Egr-1 zinc-finger–DNA complex. In this approach, transverse and longitudinal cross-correlation rates for 15N shift anisotropy 15N–1H dipole–dipole relaxation interference were analyzed together heteronuclear Overhauser effect data...
To explore the antiviral efficacy, safety and protective ability against mother-to-infant transmission of telbivudine in pregnant patients with chronic hepatitis B (CHB) during first trimester.Eighty four gravid women who were diagnosed CHB, their trimester pregnancy, had refused to terminate pregnancies enrolled; all study participants clinically classified as active cases positivity for both surface antigen (HBsAg) e (HBeAg), HBV DNA more than or equal 107 copies/mL serum level alanine...