A5004271560- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Renal and related cancers
- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- Cellular Mechanics and Interactions
- Hippo pathway signaling and YAP/TAZ
- Mitochondrial Function and Pathology
- Cytokine Signaling Pathways and Interactions
- Wnt/β-catenin signaling in development and cancer
- Chromosomal and Genetic Variations
- Fibroblast Growth Factor Research
- Osteoarthritis Treatment and Mechanisms
- Medicinal Plants and Bioactive Compounds
- Advanced ceramic materials synthesis
- Metabolism and Genetic Disorders
- Industrial Vision Systems and Defect Detection
- ATP Synthase and ATPases Research
- Plant Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- Biochemical Analysis and Sensing Techniques
- RNA Research and Splicing
- Genetic Syndromes and Imprinting
- Caveolin-1 and cellular processes
Kyushu University
2017-2025
University of Yamanashi
2022-2024
Helmholtz Zentrum München
2017
Center for Environmental Health
2017
Centre National de la Recherche Scientifique
2013-2015
Inserm
2013-2015
Université de Strasbourg
2014
Institut de génétique et de biologie moléculaire et cellulaire
2013
Kyoto University
2009
Nagoya University
2006
The fusion of the gametes upon fertilization results in formation a totipotent cell. Embryonic chromatin is expected to be able support large degree plasticity. However, whether this plasticity relies on particular conformation embryonic unknown. Moreover, functionally linked cellular potency has not been addressed. Here, we adapted fluorescence recovery after photobleaching (FRAP) developing mouse embryo and show that mobility core histones H2A, H3.1, H3.2 unusually high two-cell stage...
The zonula adherens (ZA), a type of junction (AJ), plays major role in epithelial cell–cell adhesions. It remains unknown how the ZA is remodeled during reorganization. Here we found that was converted to another AJ with punctate morphology (pAJ) at margins colonies. F-actin–stabilizing protein EPLIN (epithelial lost neoplasm), which functions maintain via its association αE-catenin, pAJs. Consistently, fusion αE-catenin and contributed formation but not We show junctional tension important...
Significance Polarization is essential for epithelial cells to exert a variety of functions. Epithelial polarization includes characteristic microtubule array formation. The microtubules are oriented along the apicobasal axis with their minus ends facing apically. molecules that regulate such epithelial-specific assembly remain unknown, however. Our study demonstrates in intestinal cells, minus-end binding protein CAMSAP3 (calmodulin-regulated–spectrin-associated 3) tethers noncentrosomal...
Abstract Establishment of the DNA methylation landscape mammalian oocytes, mediated by DNMT3A-DNMT3L complex, is crucial for reproduction and development. In mouse high levels occur exclusively in transcriptionally active regions, with moderate to low other regions. Histone H3K36me3 mediates transcribed regions; however, it unknown which histone mark guides Here, we show that, H3K36me2 highly enriched X chromosome broadly distributed across all autosomes. Upon depletion, moderately...
Zygotic genome activation (ZGA) after fertilization enables the maternal-to-zygotic transition. However, global view of ZGA, particularly at initiation, is incompletely understood. Here, we develop a method to capture and sequence newly synthesized RNA in early mouse embryos, providing transcriptional reprogramming during ZGA. Our data demonstrate that major ZGA gene begins earlier than previously thought. Furthermore, identify set genes activated minor promoters which show enrichment Obox...
Compartmentalization of the plasma membrane in a cell is fundamental for its proper functions. In this study, we present evidence that mammalian Fat4 and Dachsous1 cadherins regulate apical organization embryonic cerebral cortex. neural progenitor cells cortex, were concentrated together cell–cell contact area positioned more apically than adherens junction (AJ). These molecules interacted heterophilic fashion, affecting their respective protein levels. We further found associated...
Transcription of human mitochondrial DNA (mtDNA) begins from specific transcription promoters. In strand-asynchronous mtDNA replication, transcripts the light-strand promoter serve as primers for leading-strand synthesis at origin H-strand replication (OH). A 7S strand, a presumed aborted product, is also synthesized OH. Transition RNA to OH crucial balancing with transcription, yet mechanism remains unclear. Herein, we examine role elongation factor (TEFM) in this process. TEFM knockout...
Abstract Maintaining biodiversity is an essential task, but storing germ cells as genetic resources using liquid nitrogen difficult, expensive, and easily disrupted during disasters. Our aim to generate cloned mice from freeze-dried somatic cell nuclei, preserved at −30 °C for up 9 months after freeze drying treatment. All died drying, nucleic DNA damage significantly increased. However, nuclear transfer, we produced blastocysts cells, established transfer embryonic stem lines. Using these...
Highlights•A forward genetic screen identifies Zfp281 as a factor for ES-to-TS-like conversion•Zfp281 shapes the transcriptome of TS cells and regulates early placental development•Zfp281 interacts with MLL or COMPASS subunits binds to promoters target genes•ZNF281 stabilizes in undifferentiated human cellsSummaryPlacental development is key event mammalian reproduction embryogenesis. However, molecular basis underlying not fully understood. Here, we conduct identify regulators...
ABSTRACT Round spermatid injection (ROSI) results in a lower birth rate than intracytoplasmic sperm injection, which has hampered its clinical application. Inefficient development of ROSI embryos been attributed to epigenetic abnormalities. However, the chromatin-based mechanism that underpins low remains be determined. Here, we show repressive histone mark, H3K27me3, persists from mouse round spermatids into zygotes and spermatid-derived H3K27me3 is associated with less accessible chromatin...
YY1 plays multifaceted roles in various cell types. We recently reported that regulates nucleosome organization early mouse embryos. However, despite the impaired absence of YY1, transcriptome was minimally affected eight-cell then hypothesized might prepare a chromatin environment to regulate gene expression at later stages. To test this possibility, we performed analysis morula stage. found substantial number genes are aberrantly expressed YY1. Furthermore, our revealed is required for...
Nucleosome positioning can alter the accessibility of DNA-binding proteins to their cognate DNA elements, and thus its precise control is essential for cell identity function. Mammalian preimplantation embryos undergo temporal changes in gene expression potency, suggesting involvement dynamic epigenetic during this developmental phase. However, dynamics nucleosome organization early development are poorly understood. In study, using a low-input MNase-seq method, we show that globally obscure...
Willin is a FERM‐domain protein, which related to the Drosophila Expanded, protein known be component of Hippo signaling pathway. We recently showed that localizes at apical junctional complex (AJC) in epithelial cells together with Par3 and regulates contractility circumferential actomyosin cables by recruiting aPKC AJC. However, it remains unresolved how becomes associated Here, we report binds nectins, Ig‐family proteins, also localize AJC via their homophilic or heterophilic...
Histone lysine-to-methionine (K-to-M) mutations have been identified as driver in human cancers. Interestingly, these 'oncohistone' inhibit the activity of histone methyltransferases. Therefore, they can potentially be used versatile tools to investigate roles modifications. In this study, we generated a genetically engineered mouse line which an H3.3K36M mutation could induced endogenous H3f3b gene. Since has causative chondroblastoma, chondrocyte lineage embryonic limbs. We found that...
Regulation of gene expression through histone modifications underlies cell homeostasis and differentiation. Kdm4d Kdm4dl exhibit a high degree similarity demethylate H3K9me3. However, the physiological functions these proteins remain unclear. In this study, we generated mutant mice found that was dispensable for mouse development. generation mice, unexpectedly male were subfertile because impaired sperm motility. The absence associated with an altered distribution H3K9me3 in round...