A5050221085- Lipid Membrane Structure and Behavior
- Toxin Mechanisms and Immunotoxins
- Photochemistry and Electron Transfer Studies
- Protein Structure and Dynamics
- Clostridium difficile and Clostridium perfringens research
- Free Radicals and Antioxidants
- Molecular Sensors and Ion Detection
- Photochromic and Fluorescence Chemistry
- Seaweed-derived Bioactive Compounds
- Organic Chemistry Cycloaddition Reactions
- Protein Interaction Studies and Fluorescence Analysis
- Chemical Reactions and Mechanisms
- Ion channel regulation and function
- Electron Spin Resonance Studies
- Escherichia coli research studies
- Lanthanide and Transition Metal Complexes
- Nasal Surgery and Airway Studies
- RNA Interference and Gene Delivery
- Sinusitis and nasal conditions
- Erythrocyte Function and Pathophysiology
- Food Industry and Aquatic Biology
- Protein purification and stability
- DNA and Nucleic Acid Chemistry
- Inorganic and Organometallic Chemistry
- Electrochemical Analysis and Applications
Kharkiv National Medical University
2020-2025
National Technical University "Kharkiv Polytechnic Institute"
2017-2025
V. N. Karazin Kharkiv National University
2000-2016
University of Kansas Medical Center
2005-2014
University Medical Center
2005-2010
University of Kansas
2008
Polish Academy of Sciences
2001-2007
Institute of Physical Chemistry
2006-2007
Université d'Avignon et des Pays de Vaucluse
2006
Institut de Biologie Physico-Chimique
2006
Luminescent properties of several ortho-hydroxy derivatives 2,5-diphenyl-1,3,4-oxadiazole were studied in solvents different polarity and protolytic ability compared with the analogous 1,3-oxazole, by us earlier. Evaluations rate constants for excited state intramolecular proton transfer (ESIPT) reaction radiationless degradation energy electronic excitation ESIPT product, phototautomer form, made on basis fluorescence quantum yields kinetic measurements. The phototransfer is most efficient...
The pH-triggered membrane insertion pathway of the T-domain diphtheria toxin was studied using site-selective fluorescence labeling with subsequent application several spectroscopic techniques (e.g., correlation spectroscopy, FRET, lifetime quenching, and kinetic fluorescence). FCS measurements indicate that pH-dependent formation membrane-competent form depends only slightly on amount anionic lipids in membrane. transbilayer insertion, however, is strongly favored by lipids. Kinetic FRET...
Hemifluorinated compounds, such as HF-TAC, make up a novel class of nondetergent surfactants designed to keep membrane proteins soluble under nondissociating conditions [Breyton, C., et al. (2004) FEBS Lett. 564, 312]. Because fluorinated and hydrogenated chains do not mix well, supramicellar concentrations these can coexist with intact lipid vesicles. To test the ability HF-TAC assist proper insertion proteins, we examined its effect on pH-triggered diphtheria toxin T-domain. The function...
Experimental determination of the free energy stabilizing structure membrane proteins in their native lipid environment is undermined by lack appropriate methods and suitable model systems. Annexin B12 (ANX) a soluble protein which reversibly inserts into membranes under mildly acidic conditions, makes it good experimental for thermodynamic studies folding stability proteins. Here we apply fluorescence correlation spectroscopy quantitative analysis ANX partitioning large unilamellar vesicles...
The safety of food additives E407 and E407a has raised concerns in the scientific community. Thus, this study aims to assess local systemic toxic effects common additive rats orally exposed it for two weeks. Complex evaluations semi-refined carrageenan (E407a) on upon oral exposure were performed. Local intestine analyzed using routine histological stains CD68 immunostaining. Furthermore, circulating levels inflammatory markers assessed. A fluorescent probe O1O (2-...
<p><strong>Aim<br /></strong> To investigate the lipid membranes of rat enterocytes in chronic carrageenan-induced gastroenterocolitis accompanied by activation apoptotic processes. <br /><strong>Methods<br Steady-state fluorescence spectroscopy: a study fluorescent probes-by ortho-hydroxy derivatives 2,5-diaryl-1,3oxazole. Activity apoptosis signal-regulating kinase 1 and poly (ADP-ribose) polymerase...
Abstract Thioacetylacetone and its variously deuterated isotopomers have been investigated using electronic vibrational spectroscopy combined with quantum chemical calculations. is known for photochromic properties, but the structures of initial final forms subject a long debate. Analysis IR spectra recorded in low‐temperature argon xenon matrices, room‐temperature solutions, gas phase has allowed us to establish nature species precursor. Similar case another β ‐thioxoketone,...