A5035117800- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Antibiotic Resistance in Bacteria
- Tissue Engineering and Regenerative Medicine
- Graphene and Nanomaterials Applications
- Antimicrobial Resistance in Staphylococcus
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- Antibiotics Pharmacokinetics and Efficacy
- Pneumonia and Respiratory Infections
- Antibiotic Use and Resistance
- Craniofacial Disorders and Treatments
- Bacterial biofilms and quorum sensing
- RNA Interference and Gene Delivery
- Bone Metabolism and Diseases
- Bacterial Identification and Susceptibility Testing
- Bone and Dental Protein Studies
- Advanced biosensing and bioanalysis techniques
- Bone health and treatments
- Microbial Metabolism and Applications
- Cleft Lip and Palate Research
- Pediatric health and respiratory diseases
- Epigenetics and DNA Methylation
- 3D Printing in Biomedical Research
- Cancer Diagnosis and Treatment
University of Sioux Falls
2016-2024
University of South Dakota
2015-2024
University of Michigan
2009-2024
Peking Union Medical College Hospital
2009-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2009-2024
University of Iowa
2018-2024
Chinese Academy of Sciences
2006-2024
Suzhou Institute of Nano-tech and Nano-bionics
2024
Shandong University of Science and Technology
2021-2022
Wuxi People's Hospital
2010-2019
For the first time, electrospun polycaprolactone (PCL) 3D nanofibrous scaffold has been developed by an innovative and convenient approach (i.e., thermally induced nanofiber self‐agglomeration followed freeze drying), possesses interconnected hierarchically structured pores including macropores with sizes up to ≈300 μm. The novel PCL is soft elastic very high porosity of ≈96.4%, thus it morphologically/structurally similar natural extracellular matrix well suited for cell functions tissue...
Novel core−shell structured nanoassemblies are assembled by a β-cyclodextrin containing positively charged host polymer and hydrophobic guest polymer. The core of these types assemblies serves as nanocontainer to load release the drugs, while hydrophilic shell is able condense plasmid DNA achieve its transfection/expression in osteoblast cells. These may be used new generation multifunctional nanocarriers for simultaneous drug delivery gene therapy.
Carbapenem resistance in Acinetobacter spp. is an emerging problem China. We investigated the molecular epidemiology and carbapenemase genes of 221 nonrepetitive imipenem-resistant clinical isolates collected from 1999 to 2005 at 11 teaching hospitals Genotyping by pulsed-field gel electrophoresis (PFGE) found 15 PFGE patterns. Of these, one (clone P) was identified four Beijing another A) geographically disparate cities. Most exhibited high-level all beta-lactams were only susceptible...
The role of erythropoietin (Epo) and Epo/Epo receptor (EpoR) signaling pathways for production red blood cells are well established. However, little is known about Epo/EpoR in non-hematopoietic cells. Recently, we demonstrated that Epo activates JAK/STAT hematopoietic stem (HSCs), leading to the bone morphogenetic protein 2 (BMP2) formation also directly mesenchymal form osteoblasts vitro. In this study, investigated effects mTOR on Epo-mediated osteoblastogenesis osteoclastogenesis. We...
The purpose of this study was to determine the lineage progression human and murine very small embryonic-like (HuVSEL or MuVSEL) cells in vitro vivo. In vitro, HuVSEL MuVSEL differentiated into all three embryonic germ layers. produced robust mineralized tissue origin compared with controls calvarial defects. Immunohistochemistry demonstrated that gave rise neurons, adipocytes, chondrocytes, osteoblasts within were also able differentiate similar lineages. First round serial transplants...
Abstract Nuclear receptor peroxisome proliferator-activated receptor-γ (PPARγ) is an essential transcription factor for adipocyte differentiation. In mesenchymal stem cells, PPARγ has been assumed to play a negative role in osteoblastic differentiation, by working adipogenesis dependent manner, due the reciprocal relationship between osteoblast and However, direct of function not fully understood, part inadequate model systems. Here, we describe adenoviral-mediated knockout system which...
Utilizing biomimetic materials to potentiate endogenous cell growth or signaling is superior relying on exogenous cells signals for bone formation. Desferoxamine (DFO), which a hypoxia-mimetic agent that chelates iron (Fe3+), mimics hypoxia encourage healing. However, high cytotoxicity, off-target effects, and the short half-life of DFO have significantly impeded its further applications. We mitigated these side effects by locally immobilizing onto gelatin nanofibrous (GF) scaffold retained...
Abstract The advent of adaptive manufacturing techniques supports the vision cell‐instructive materials that mimic biological tissues. 3D jet writing, a modified electrospinning process reported herein, yields structures with unprecedented precision and resolution offering customizable pore geometries scalability to over tens centimeters. These scaffolds support expansion differentiation human mesenchymal stem cells in vitro. Implantation these constructs leads healing critical bone defects...
// Fang 1 , Ashley VanCleave Ralph Helmuth 1, 2 Haydee Torres 3 Kirby Rickel 4 Hannah Wollenzien 5 Hongli Sun 6 Erliang Zeng 7 Jing Zhao 8, 9 and Jianning Tao 3, 5, 10 Cancer Biology Immunotherapies Group, Sanford Research, Sioux Falls, SD, USA BRIN Scholar from Dakota Wesleyan University, Department of Chemistry Biochemistry, South State Brookings, Biomedical Engineering, University Dakota, Basic Sciences, Vermillion, Oral Maxillofacial Surgery, Iowa, Iowa City, IA, Departments Preventive &...
The acquisition of resistance to one antibiotic sometimes leads collateral sensitivity a second antibiotic. Here, we show that vancomycin in Enterococcus faecium is associated with remarkable increase susceptibility pleuromutilin antibiotics (such as lefamulin), which target the bacterial ribosome. trade-off between and pleuromutilins mediated by epistasis van gene cluster msrC, encoding an ABC-F protein protects ribosomes from targeting. In mouse models vancomycin-resistant E. colonization...
ABSTRACT The resistance mechanism of 49 Enterobacteriaceae isolates with decreased susceptibility to carbapenems collected from 2004 2008 at 16 teaching hospitals in China was investigated. Moderate- high-level carbapenem most more closely associated loss or expression both major porins combined production AmpC extended-spectrum β-lactamase enzymes, while KPC-2, IMP-4, and IMP-8 carbapenemase may lead a low moderate level China.
To demonstrate the pro-osteogenic effect of burn injury on heterotopic bone formation using a novel ossicle in vivo model.Heterotopic ossification (HO), or abnormal soft tissue, is troubling sequela and trauma injuries. The exact mechanism by which influences unknown. aim this study was to develop mouse model formation. We hypothesized that would enhance early vascularization subsequent subcutaneously implanted mesenchymal stem cells.Mouse adipose-derived cells were harvested from C57/BL6...