A5102954422- Identification and Quantification in Food
- Fish Biology and Ecology Studies
- Cancer Cells and Metastasis
- Chemokine receptors and signaling
- Ichthyology and Marine Biology
- Bone health and treatments
- Prostate Cancer Treatment and Research
- Tuberculosis Research and Epidemiology
- Immunotherapy and Immune Responses
- Marine and fisheries research
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Cancer, Stress, Anesthesia, and Immune Response
- 3D Printing in Biomedical Research
- Genetic diversity and population structure
- Cancer Mechanisms and Therapy
- PI3K/AKT/mTOR signaling in cancer
- Immunodeficiency and Autoimmune Disorders
- Advanced Battery Materials and Technologies
- Advanced Battery Technologies Research
- Diagnosis and treatment of tuberculosis
- Marine Bivalve and Aquaculture Studies
- Aquaculture Nutrition and Growth
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
University of California, Los Angeles
2018-2024
APLA Health
2020
BioSurfaces (United States)
2014-2018
University of Michigan
2005-2018
Henry Ford Hospital
2012-2013
Pukyong National University
2008-2012
National Institute of Fisheries Science
2006-2007
Korea Research Institute of Bioscience and Biotechnology
1997-2005
Samsung Medical Center
2000
Sungkyunkwan University
2000
HSC homing, quiescence, and self-renewal depend on the bone marrow niche. A large proportion of solid tumor metastases are metastases, known to usurp homing pathways establish footholds in marrow. However, it is not clear whether tumors target niche during metastasis. Here we have shown a mouse model metastasis that human prostate cancer (PCa) cells directly compete with HSCs for occupancy Importantly, increasing size promoted metastasis, whereas decreasing compromised dissemination....
Human embryonic stem cells (hESCs) have great potentials for future cell-based therapeutics. However, their mechanosensitivity to biophysical signals from the cellular microenvironment is not well characterized. Here we introduced an effective microfabrication strategy accurate control and patterning of nanoroughness on glass surfaces. Our results demonstrated that nanotopography could provide a potent regulatory signal over different hESC behaviors, including cell morphology, adhesion,...
Abstract Nuclear receptor peroxisome proliferator-activated receptor-γ (PPARγ) is an essential transcription factor for adipocyte differentiation. In mesenchymal stem cells, PPARγ has been assumed to play a negative role in osteoblastic differentiation, by working adipogenesis dependent manner, due the reciprocal relationship between osteoblast and However, direct of function not fully understood, part inadequate model systems. Here, we describe adenoviral-mediated knockout system which...
Abstract Self-renewal of human embryonic stem cells and induced pluripotent (hiPSCs)—known as (PSC)—is influenced by culture conditions, including the substrate on which they are grown. However, details molecular mechanisms interconnecting self-renewal these remain unclear. We describe a signaling pathway in hPSCs linking expression pluripotency transcription factors to integrin α6β1 inactivation focal adhesion kinase (FAK). Disruption this results hPSC differentiation. In hPSCs, is dominant...
Mammalian EAK-7 forms an alternative mTOR complex.
Bone is the preferred metastasis site of advanced prostate cancer (PCa). Using an in vivo murine model human PCa cell to bone, we noted that majority animals develop skeletal have either spinal lesions or bones hindlimb. Much less frequently, forelimb. We therefore speculated whether environment forelimb not permissive for growth PCa. Consequently, data on tumor prevalence were normalized account number cells arriving after intravascular injection, marrow cellularity, and hematopoietic stem...
There is evidence that cancer stem-like cells (CSC) and neuroendocrine behavior play critical roles in the pathogenesis clinical course of metastatic castration-resistant prostate (m-CRPC). However, there limited mechanistic understanding how CSC phenotypes impact development m-CRPC. In this study, we explored role intracellular chemokine CXCL12γ induction differentiation its on expression was detected small-cell carcinoma tissues circulating tumor from m-CRPC patients displaying an...
Growth-factor-free bone regeneration remains a challenge in craniofacial engineering. Here, we engineered an osteogenic niche composed of commercially modified alginate hydrogel and whitlockite microparticles (WHMPs), which impart tunable physicochemical properties that can direct osteogenesis human gingival mesenchymal stem cells (GMSCs). Our vitro studies demonstrate WHMPs induce GMSCs more effectively than previously demonstrated hydroxyapatite (HApMPs). Alginate–WHMP hydrogels showed...
Prostate cancer metastases and hematopoietic stem cells (HSC) frequently home to the bone marrow, where they compete occupy same HSC niche. We have also shown that under conditions of stress, HSCs secrete morphogenetic proteins (BMP)-2 BMP-6 drives osteoblastic differentiation from mesenchymal precursors. As it is not known, we examined whether metastatic prostate can alter regulation normal formation by progenitor (HPC). HSC/HPCs isolated mice bearing nonmetastatic tumor were their ability...
Abstract Although a specific group of transcription factors such as OCT4, SOX2, and NANOG are known to play essential roles in pluripotent stem cell (PSC) self-renewal, pluripotency, reprogramming, other the key signaling pathways regulating these important properties not completely understood. Here, we demonstrate that PSC marker Developmental Pluripotency Associated 5 (DPPA5) plays an role human (hPSC) self-renewal reprogramming feeder-free conditions. Compared hPSCs grown on mouse...
Abstract Therapeutic strategies targeting both cancer cells and associated in the tumor microenvironment offer significant promise therapy. We previously reported that generation 5 (G5) dendrimers conjugated with cyclic‐RGD peptides target expressing integrin alpha V beta 3. In this study, we report a novel dendrimer conjugate modified to deliver mammalian of rapamycin (mTOR) inhibitor, rapamycin. vitro analyses demonstrated drug conjugate, G5‐FI‐RGD‐rapamycin, binds prostate (PCa)...
Regulation of mTOR signaling depends on an intricate interplay post-translational protein modification. Recently, mEAK-7 (mTOR associated protein, eak-7 homolog) was identified as a positive activator via alternative complex. However, the upstream regulation in human cells is not known. Because microRNAs are capable modulating translation RNA eukaryotes, we conducted bioinformatic search for relevant targeting using Exiqon miRSearch V3.0 algorithm. Based score obtained through V3.0, top...
Abstract Genetic variation was surveyed at the mitochondrial control region (766bp) to test for presence of genetic stock structure in small yellow croaker, Larimichthys polyactis from Yellow and East China Seas. Individuals croaker could not be distinguished on basis its location, as demonstrated using neighbor-joining (NJ) method, unweighted pair-group arithmetic average (UPGMA) minimum spanning network (MSN). Analysis molecular variance revealed no significant differences among...
We describe a platform to derive, culture, and differentiate genomically stable, transgene-free human induced pluripotent stem cells (iPSCs) on fully synthetic polymer substrate made of grafted zwitterionic hydrogel: poly2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide (PMEDSAH). Three independent iPSC lines derived in these conditions demonstrated continuous self-renewal, genomic stability, pluripotency vitro vivo after up 9 months culture PMEDSAH-grafted plates....
본 연구는 여름철 고온기에 작물을 정상적으로 재배할 수 있는 환경을 조성하여 농산 가격이 가장 높은 여름철에 수확량을 높이고 재배기간을 연장시켜 농가소득을 올릴 방안을 제시하고자 수행하였다. 바닥면적 504m2의 연동온실의 최대 냉방부하는 462,609W로 나타났으며, 온실을 차광하지 않고도 32℃ 이하로 유지하기 위해서는 시간당 472L의 물을 포그 분무해야 하는 것으로 나타났다. 냉방시스템은 장치, 유동팬, 차광장치로 구성하고, 이들 장치를 효과적으로 제어할 냉방 자동제어장치를 개발하였다. 냉방시스템의 성능은 온실 외기온 보다 내부온도를 6℃ 낮출 온실의 내부 상대습도는 주간에는 40~80%로 대조 20~60% 약 20% 높게 나타나 오이의 생육에 기여하는 생육상태는 온실에서 재배한 오이가 온실에 비해 초장, 엽장, 엽폭, 엽수, 엽록소 값이 전체적으로 오이 수확량은 단동 온실에서는 1.8배, 연동 2배