A5008281781- Biotin and Related Studies
- Mitochondrial Function and Pathology
- Genetics, Aging, and Longevity in Model Organisms
- Epigenetics and DNA Methylation
- Click Chemistry and Applications
- RNA modifications and cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Cellular transport and secretion
- Immune cells in cancer
- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- Metabolism, Diabetes, and Cancer
- Cell Image Analysis Techniques
- Ethics in Clinical Research
- Ubiquitin and proteasome pathways
- Biochemical Acid Research Studies
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Hematopoietic Stem Cell Transplantation
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- Diet and metabolism studies
Leibniz Institute on Aging - Fritz Lipmann Institute (FLI)
2019-2024
Ribosome biogenesis is initiated by RNA polymerase I (Pol I)-mediated synthesis of pre-ribosomal (pre-rRNA). Pol activity was previously linked to longevity, but the underlying mechanisms were not studied beyond effects on nucleolar structure and protein translation. Here we use multi-omics functional tests show that curtailment remodels lipidome preserves mitochondrial function promote longevity in Caenorhabditis elegans. Reduced pre-rRNA improves energy homeostasis metabolic plasticity...
Preconditioning with lipopolysaccharide (LPS) induces neuroprotection against subsequent cerebral ischemic injury, mainly involving innate immune pathways. Microglia are resident cells of the central nervous system (CNS) that respond early to danger signals through memory-like differential reprogramming. However, cell-specific molecular mechanisms underlying preconditioning not fully understood. To elucidate distinct on microglia, we compared these proteomic profiles in response LPS and...
Recurrently mutated in lymphoid neoplasms, the transcription factor RFX7 is emerging as a tumor suppressor. Previous reports suggested that may also have role neurological and metabolic disorders. We recently reported responds to p53 signaling cellular stress. Furthermore, we found target genes be dysregulated numerous cancer types beyond hematological system. However, our understanding of RFX7's gene network its health disease remains limited. Here, generated knock-out cells employed...
Abstract Hematopoietic stem cell (HSC) transplantation is successfully applied since the late 1950s. However, its efficacy can be impaired by insufficient numbers of donor HSCs. A promising strategy to overcome this hurdle use an advanced ex vivo culture system that supports proliferation and, at same time, maintains pluripotency Therefore, we have developed artificial 3D bone marrow-like scaffolds made polydimethylsiloxane (PDMS) model natural HSC niche in vitro. These PDMS combination with...
Proximity-dependent biotinylation is an important method to study protein-protein interactions in cells, for which expanding number of applications has been proposed. The laborious and time-consuming sample processing limited project sizes so far. Here, we introduce automated workflow on a liquid handler process up 96 samples at time. automation not only allows higher numbers be processed parallel but also improves reproducibility lowers the minimal input. Furthermore, combined with shorter...
Summary The diabetes drug metformin is to be clinically tested in aged humans achieve health span extension, but little known about responses of old non-diabetic individuals this drug. By vitro and vivo tests we found that shortens life limits cell survival when provided late life, contrary its positive early effects. Mechanistically, exacerbates aging-associated mitochondrial dysfunction towards respiratory failure, aggravated by the inability cells upregulate glycolysis response metformin,...
We introduce an automated workflow for proximity dependent biotinylation on a liquid handler to process up 96 samples at time,combined with shorter chromatography gradients and data-independent acquisition increase analysis throughput enable reproducible protein quantitation
We introduce an automated workflow for proximity dependent biotinylation on a liquid handler to process up 96 samples at time,combined with shorter chromatography gradients and data-independent acquisition increase analysis throughput enable reproducible protein quantitation
Abstract Preconditioning with LPS induces neuroprotection against subsequent cerebral ischemic injury, mainly involving innate immune pathways. Microglia are CNS-resident cells that respond early to danger signals through memory-like differential reprogramming. However, the cell-specific molecular mechanisms underlying preconditioning not fully understood. To elucidate distinct of on microglia, we compared these proteomic profiles in response and without transient focal brain ischemia...
Abstract Proximity dependent biotinylation is an important method to study protein-protein interactions in cells, for which expanding number of applications has been proposed. The laborious and time consuming sample processing limited project sizes so far. Here, we introduce automated workflow on a liquid handler process up 96 samples at time. automation does not only allow higher numbers be processed parallel, but also improves reproducibility lowers the minimal input. Furthermore, combined...
Background: The mammalian target of Rapamycin complex 1 (mTORC1) increases cell size by initiating translation as well inhibiting catabolic functions such proteolysis or autophagy. We have previously proposed Proline-rich Akt substrate (Pras40) a cardioprotective endogenous inhibitor mTOR-dependent protein synthesis during pathologic growth. Pras40 is released from mTORC1 growth, but other interactions are largely unknown. In proteomic screen we now found novel interaction with the 26S...
Abstract Recurrently mutated in lymphoid neoplasms, the transcription factor RFX7 is emerging as a tumor suppressor. Previous reports suggested that may also have role neurological and metabolic disorders. We recently reported responds to p53 signaling cellular stress. Furthermore, we found target genes be dysregulated numerous cancer types beyond hematological system. However, our understanding of RFX7’s gene network its health disease remains limited. Here, generated knock-out cells...