A5073516006- Kruppel-like factors research
- Biosensors and Analytical Detection
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Lipid metabolism and biosynthesis
- Peroxisome Proliferator-Activated Receptors
- Genetic Syndromes and Imprinting
- Adipose Tissue and Metabolism
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Fibroblast Growth Factor Research
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Malaria Research and Control
- Cancer-related gene regulation
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Animal Disease Management and Epidemiology
- Virology and Viral Diseases
- Sepsis Diagnosis and Treatment
- SARS-CoV-2 detection and testing
- Endoplasmic Reticulum Stress and Disease
- Diphtheria, Corynebacterium, and Tetanus
- Genetic Mapping and Diversity in Plants and Animals
- Reproductive tract infections research
University of the Sunshine Coast
2018-2025
Defence Materials Technology Centre (Australia)
2022-2023
ACT Government
2019-2021
The University of Queensland
2018-2021
Commonwealth Scientific and Industrial Research Organisation
2019-2021
University of Graz
2011-2019
Howard Hughes Medical Institute
2015
New York University
2015
Temple University
2015
Cardiac triacylglycerol (TG) catabolism critically depends on the TG hydrolytic activity of adipose triglyceride lipase (ATGL). Perilipin 5 (Plin5) is expressed in cardiac muscle (CM) and has been shown to interact with ATGL its coactivator comparative gene identification-58 (CGI-58). Furthermore, ectopic Plin5 expression increases cellular content Plin5-deficient mice exhibit reduced levels. In this study we show that muscle-specific overexpression perilipin (CM-Plin5) massively accumulate...
Krüppel-like factors (KLFs) are deoxyribonucleic acid–binding transcriptional that regulate various pathways control metabolism and other cellular mechanisms. Various KLF isoforms have been associated with cellular, organ, or systemic metabolism. Altered expression activation of KLFs has linked to metabolic abnormalities, such as obesity diabetes, well heart failure. This review article summarizes the functions KLFs, networks different jointly in health disease.
Fatty acid oxidation is transcriptionally regulated by peroxisome proliferator-activated receptor (PPAR)α and under normal conditions accounts for 70% of cardiac ATP content. Reduced Ppara expression during sepsis heart failure leads to reduced fatty myocardial energy deficiency. Many the transcriptional regulators are unknown.To determine role Krüppel-like factor 5 (KLF5) in regulation Ppara.We discovered that KLF5 activates gene via direct promoter binding. This blocked hearts septic mice...
The efficient and accurate diagnosis of dengue, a major mosquito-borne disease, is primary importance for clinical care, surveillance, outbreak control. identification specific dengue virus serotype 1 (DENV-1) to DENV-4 can help in understanding the transmission dynamics spread disease. four rapid low-resource serotype-specific tests use simple sample preparation reagent followed by reverse transcription-isothermal recombinase polymerase amplification (RT-RPA) combined with lateral flow...
Accurate and timely diagnosis of Nipah virus (NiV) requires rapid, inexpensive, robust diagnostic tests to control spread disease. Current state the art technologies are slow require laboratory infrastructure that may not be available in all endemic settings. Here we report development comparison three rapid NiV molecular based on reverse transcription recombinase-based isothermal amplification coupled with lateral flow detection. These include a simple fast one-step sample processing step...
Fibroblast growth factor 21 (FGF21) is a PPARα-regulated gene elucidated in the liver of PPARα-deficient mice or PPARα agonist-treated mice. Mice globally lacking adipose triglyceride lipase (ATGL) exhibit marked defect TG catabolism associated with impaired PPARα-activated expression heart and liver, including drastic reduction hepatic FGF21 mRNA expression. Here we show that markedly increased ATGL-deficient accompanied by elevated endoplasmic reticulum (ER) stress markers, which can be...
Efficient catabolism of cellular triacylglycerol (TG) stores requires the TG hydrolytic activity adipose triglyceride lipase (ATGL). The presence comparative gene identification-58 (CGI-58) strongly increased ATGL-mediated in cell culture experiments. Mutations genes coding for ATGL or CGI-58 humans cause neutral lipid storage disease characterized by accumulation multiple tissues. mutations a severe phenotype especially cardiac muscle leading to cardiomyopathy that can be lethal. In...
Nucleic acid analysis has become highly relevant for point-of-care (POC) diagnostics since the advent of isothermal amplification methods that do not require thermal cycling. In particular, recombinase polymerase (RPA) combined with lateral flow detection offers a rapid and simple solution field-amenable low-resource nucleic testing. Expanding POC tests multiple analytes is vital to improve diagnostic efficiency because increased multiplexing capacity enables higher information density...
Chlamydia pecorum is a significant cause of morbidity and mortality in koalas major contributor to population decline. Due its crucial role vaccine development use as strain typing tool, multiple studies have investigated the prevalence diversity C. outer membrane protein A (MOMP), encoded by ompA. This prior work has shown that ompA genotypes vary across geographical regions, with identified Eastern Australia. Despite these earlier studies, our understanding distribution MOMP remains...
<h3>Background & Aims</h3> Adipose tissue (AT)-derived fatty acids (FAs) are utilized for hepatic triacylglycerol (TG) generation upon fasting. However, their potential impact as signaling molecules is not established. Herein we examined the role of exogenous AT-derived FAs in regulation gene expression by investigating mice with a defect FA supply to liver. <h3>Methods</h3> Plasma levels, TG hydrolytic activities and lipid content were determined lacking lipase co-activator comparative...
The 2014/2015 Ebolavirus outbreak resulted in more than 28,000 cases and 11,323 reported deaths, as of March 2016. Domestic transmission the Guinea strain associated with occurred mainly six African countries, international was four countries. Outbreak management limited by inability to rapidly diagnose infected cases. A further fifteen countries Africa are predicted be at risk outbreaks future a consequence climate change urbanization. Early detection reduction rates is critical prevent...
Efficient and accurate diagnosis of Hendra virus (HeV), a biosafety level 4 (BSL-4) pathogen zoonotic disease, is primary importance for surveillance outbreak control in the Australian equine industry. Sporadic HeV spillover events pose serious public health concern are predicted to expand geographically, aligning with moving distribution main reservoir hosts, flying-foxes. Here we describe development low-resource rapid test. The test used fast simple sample processing protocol followed by...
Deoxyribozymes (DNAzymes) have demonstrated a significant capacity for biocomputing and hold promise information processing within advanced biological devices if several key capabilities are developed. One required capability is reuse-having DNAzyme logic gates be cyclically, controllably, activated deactivated. We designed an oligonucleotide-based system reuse that could (1) remove previously bound inputs by addition of complementary oligonucleotides via toe-hold mediated binding (2)...
<ns4:p>The pantropic emergence of severe dengue disease can partly be attributed to the co-circulation different viruses (DENVs) in same geographical location. Effective monitoring for circulation each four DENVs is critical inform mitigation strategies. In low resource settings, this effectively achieved by utilizing inexpensive, rapid, sensitive and specific assays detect mosquito populations. study, we developed rapid DENV tests with direct applicability low-resource virus surveillance...
Abstract Background C. psittaci has recently emerged as an equine abortigenic pathogen causing significant losses to the Australian Thoroughbred industry, while Equine herpesvirus-1 (EHV-1) is a well-recognized agent. Diagnosis of these agents based on molecular assays in diagnostic laboratories. In this study, we validated and newly developed EHV-1 Loop Mediated Isothermal Amplification (LAMP) performed real-time fluorometer (rtLAMP) against reference assays. We also evaluated isothermal...
The pantropic emergence of severe dengue disease can partly be attributed to the co-circulation different viruses (DENVs) in same geographical location. Effective monitoring for circulation each four DENVs is critical inform mitigation strategies. In low resource settings, this effectively achieved by utilizing inexpensive, rapid, sensitive and specific assays detect mosquito populations. study, we developed rapid DENV tests with direct applicability low-resource virus surveillance...
RT-qPCR remains a key diagnostic methodology for COVID-19/SARS-CoV-2. Typically, nasal or saliva swabs from patients are placed in virus transport media (VTM), RNA is extracted at the pathology laboratory, and viral measured using RT-qPCR. In this study, we describe use of TNA-Cifer Reagent E pre-clinical evaluation study to inactivate SARS-CoV-2 as well prepare samples Adding 1 part 5 parts medium containing 10 min room temperature inactivated permitted detection. was compared with...
Background Viral diseases are a major problem in shrimp aquaculture facilities as these reduce growth rates, which inevitably lead to production and profit losses. Hepatopancreatic parvoviruses (HPV) common that appear be associated with high or low levels of replication specific genetic lineages. Selective breeding may result resistance HPV improved body traits such weight, meat yield colour, facilitating farming. virus titre is commonly determined by quantitative PCR (qPCR), time-consuming...