A5053679437- Respiratory viral infections research
- Malaria Research and Control
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- SARS-CoV-2 and COVID-19 Research
- Vector-borne infectious diseases
- Animal Virus Infections Studies
- Cancer-related cognitive impairment studies
- CRISPR and Genetic Engineering
- Seaweed-derived Bioactive Compounds
- Cancer-related molecular mechanisms research
- Antibiotic Resistance in Bacteria
- Immune cells in cancer
- Asthma and respiratory diseases
- Parvovirus B19 Infection Studies
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Respiratory and Cough-Related Research
- Genomics and Chromatin Dynamics
- Marine and coastal plant biology
- Brain Metastases and Treatment
- Glioma Diagnosis and Treatment
National University of Singapore
2020-2023
The University of Queensland
2013-2023
Children's Medical Research Institute
2014-2023
Queensland Children’s Hospital
2019-2023
University of Utah
2023
Children’s Health Queensland Hospital and Health Service
2013-2023
Queensland Health
2016-2023
Rice University
2022
Bridge University
2021
University of Cambridge
2019-2021
Background/Aims: Mesenchymal stem cells (MSCs) are multipotent progenitors that can differentiate into several lineages including bone. Successful bone formation requires osteogenesis and angiogenesis coupling of MSCs. Here, we investigate if simultaneous activation BMP9 Notch signaling yields effective osteogenesis-angiogenesis in Methods: Recently-characterized immortalized mouse adipose-derived (iMADs) were used as MSC source. Transgenes BMP9, NICD dnNotch1 expressed by adenoviral...
Drugs and vaccines that can interrupt the transmission of Plasmodium falciparum will be important for malaria control elimination. However, models early clinical evaluation candidate transmission-blocking interventions are currently unavailable. Here, we describe a new model evaluating from humans to Anopheles mosquitoes using controlled human infection (CHMI).Seventeen healthy malaria-naive volunteers underwent CHMI by intravenous inoculation P. falciparum-infected erythrocytes initiate...
Background. Piperaquine, coformulated with dihydroartemisinin, is a component of widely used artemisinin combination therapy. There paucity data on its antimalarial activity as single agent. Such data, if available, would inform selection new coformulations. Methods. We undertook study in healthy subjects, using the induced blood stage malaria (IBSM) model to test doses piperaquine (960, 640, and 480 mg) 3 cohorts. In pilot third cohort, gametocyte clearance following administration 15 mg,...
Overexpression of centromeric proteins has been identified in a number human malignancies, but the functional and mechanistic contributions these to disease progression have not characterized. The histone H3 variant centromere protein A (CENPA) is an epigenetic mark that determines identity. Here, using array approaches, including RNA-sequencing ChIP-sequencing analyses, immunohistochemistry-based tissue microarrays, various cell biology assays, we demonstrate CENPA highly overexpressed...
Abstract Cytokine-driven inflammation underlies the pathobiology of a wide array infectious and immune-related disorders. The TNFR-associated factor (TRAF) proteins have vital role in innate immunity by conveying signals from cell surface receptors to elicit transcriptional activation genes encoding proinflammatory cytokines. We discovered that ubiquitin E3 ligase F box component, termed Fbxo3, potently stimulates cytokine secretion human inflammatory cells mediating degradation TRAF...
Background. The role of human adenoviruses (HAdVs) in chronic respiratory disease pathogenesis is recognized. However, no studies have performed molecular sequencing HAdVs from the lower airways children with endobronchial suppuration. We thus examined major HAdV genotypes/species, and relationships to bacterial coinfection, protracted bronchitis (PBB) mild bronchiectasis (BE). Methods. Bronchoalveolar lavage (BAL) samples 245 PBB or (cylindrical) BE were included this prospective cohort...
Brain-derived neurotrophic factor (BDNF) improves cognitive function by stimulating neurogenesis and neuroplasticity. We hypothesize that higher plasma BDNF levels are protective against toxicity among adolescent young adult cancer patients (15-39 years old). In a prospective, longitudinal study, we recruited 74 newly diagnosed 118 age-matched non-cancer controls who completed the Cambridge Neuropsychological Test Automated Battery (CANTAB), Functional Assessment of Cancer Therapy-Cognitive...
Despite increasing interest in how voice assistants like Siri or Alexa might improve health care delivery and information dissemination, there is limited research assessing the quality of provided by these technologies. Voice present both opportunities risks when facilitating searches for answering health-related questions, especially now as fewer patients are seeing their physicians preventive due to ongoing pandemic. In our study, we compared 4 most widely used (Amazon Alexa, Apple Siri,...
BACKGROUNDInterventions that interrupt Plasmodium vivax transmission or eliminate dormant P. liver-stage parasites will be essential for malaria elimination. Development of these interventions has been hindered by the lack in vitro culture and could accelerated a safe reproducible clinical model malaria-naive individuals.METHODSHealthy, adults were enrolled 2 studies to assess safety, infectivity, transmissibility new isolate. Participants (Study 1, n = 2; Study 2, 24) inoculated with...
Abstract Background There is little information about cancer‐related cognitive impairment (CRCI) in adolescent and young adults (AYA, 15–39 years old) due to its rare incidence. Here, we present the pre‐treatment (before chemotherapy or radiotherapy) evaluation of function ability AYA with cancer (AYAC) a multicentered cohort study. Methods Newly diagnosed AYAC age‐matched healthy controls (HC) were recruited between 2018 2021. The primary outcome was comparison defined as 2 standard...
Evaluating the efficacy of transmission-blocking interventions relies on mosquito-feeding assays, with transmission typically assessed by microscopic identification oocysts in mosquito midguts; however, microscopy has limited throughput, sensitivity and specificity. Where low prevalence intensity infections occur, as observed during controlled human malaria infection studies or natural transmission, a reliable method for detection quantification low-level midgut is required. Here,...
The centromere is the structural unit responsible for faithful segregation of chromosomes. Although regulation centromeric function by epigenetic factors has been well-studied, contributions underlying DNA sequences have much less well defined, and existing methodologies studying genomics in biology are laborious. We identified specific markers 23 24 human chromosomes that allow rapid PCR assays capable capturing genomic landscape centromeres at a given time. Use this genetic strategy can...
Plasmodium malariae is considered a minor malaria parasite, although its global disease burden underappreciated. The aim of this study was to develop an induced blood-stage (IBSM) model P. parasite biology, diagnostic assays, and treatment.This clinical trial involved 2 healthy subjects who were intravenously inoculated with cryopreserved malariae-infected erythrocytes. Subjects treated artemether-lumefantrine after development symptoms. Prior antimalarial therapy, mosquito-feeding assays...
Plastic microfluidic devices can be rapidly and inexpensively prototyped “on the fly”, for cell culture experiments in which hydrophobic small molecules play an important role.
Microscopy and 18S qPCR are the most common field-friendly methods for quantifying malaria parasite density, it is important that these can be interpreted as giving equivalent results. We compared results of quantitative measurement Plasmodium falciparum parasitemia by microscopy in a phase 2a study. positive samples (n = 355; median 810 parasites/μL [IQR 40-10,471]) showed close agreement with mean log10/mL transformed values paired t test (difference 0.04, 95%CI - 0.01-0.10, p 0.088)....
Abstract Background Malaria transmission from humans to Anopheles mosquitoes requires the presence of gametocytes in human peripheral circulation, and dynamics are determined largely by density sex ratio gametocytes. Molecular methods thus employed measure gametocyte densities, particularly when assessing epidemiology efficacy transmission-blocking interventions. However, accurate quantification male female with molecular pure as reference standards, which not widely available. Methods...
BMP9 mediated osteogenic differentiation mechanisms of MSCs were widely explored, however, BMP9-induced angiogenesis still need to be clarified. We previously characterized that Notch1 promoted osteogenesis-angiogenesis coupling process in mesenchymal stem cells (MSCs). Here, we explored the underlying lncRNA H19 (H19) regulation through activating signaling. demonstrated basal expression level was high MSCs, and silencing attenuates osteogenesis both vitro vivo. Meanwhile, identified...
ABSTRACT Plasmodium falciparum is the most virulent human malaria parasite because of its ability to cytoadhere in microvasculature. Nonhuman primate studies demonstrated relationships among knob expression, cytoadherence, and infectivity. This has not been examined humans. Cultured clinical-grade P. parasites (NF54, 7G8, 3D7B) ex vivo -derived cell banks were characterized. Knob knob-associated histidine-rich protein CD36 adhesion, antibody recognition parasitized erythrocytes (PEs)...