A5066763713- Biological and pharmacological studies of plants
- MicroRNA in disease regulation
- Phytochemistry and Biological Activities
- Autophagy in Disease and Therapy
- Ginseng Biological Effects and Applications
- Tea Polyphenols and Effects
- Bioactive Compounds and Antitumor Agents
- Biomarkers in Disease Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Inflammatory mediators and NSAID effects
- Circular RNAs in diseases
- Systemic Lupus Erythematosus Research
- Immunodeficiency and Autoimmune Disorders
- Cytokine Signaling Pathways and Interactions
- Bioactive natural compounds
- Synthesis and Reactions of Organic Compounds
- Cancer-related Molecular Pathways
- Natural product bioactivities and synthesis
- Immune Response and Inflammation
- Immune cells in cancer
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Tannin, Tannase and Anticancer Activities
- Cancer Mechanisms and Therapy
- Essential Oils and Antimicrobial Activity
Zhejiang Chinese Medical University
2015-2024
China Academy of Chinese Medical Sciences
2018-2023
This study was designed to evaluate the pharmacological mechanisms of Aloin against gastric cancer (GC) via network pharmacology analysis combined with experimental verification.Using methods, potential targets and related GC were screened from public databases. The protein-protein interaction (PPI) network, Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment performed predict core pathways GC. expressions major predicted by in normal stomach tissues their...
Objective. To evaluate the therapeutic effect of epigallocatechin gallate (EGCG) on precancerous lesions gastric carcinoma (PLGC) and to determine whether EGCG protects against PLGC by regulating PI3K/Akt/mTOR pathway. Methods. Twenty-four male Wistar rats were randomly divided into 3 groups: normal control group (NC), model (MC), treated with (MC + EGCG). 1-Methyl-3-nitro-1-nitrosoguanidine (MNNG) sodium salicylate combined used establish rat animal model. The was evaluated body weight...
Cervical carcinoma is a leading malignant tumor among women worldwide, characterized by the dysregulation of cell cycle. Cyclin-dependent kinase 6 (CDK6) plays important roles in cycle progression, differentiation, and tumorigenesis. However, role CDK6 cervical cancer remains controversial. Here, we found that loss adenocarcinoma HeLa line inhibited proliferation but induced apoptosis as well autophagy, accompanied attenuated expression mammalian target rapamycin complex 1 (mTORC1)...
Bletilla striata is a well-known traditional Chinese herb with anti-inflammatory properties that widely used in the treatment of lung conditions such as silicosis, tuberculosis, and pneumogastric hemorrhage. However, little information on ingredients their activities available. In this study, an effect fraction (EFBS) was enriched, its underlying mechanisms were investigated. EFBS enriched by polyamide column chromatography characterized HPLC; LPS-induced acute injury model to evaluate EFBS....
Shikonin (SHK) is a pleiotropic agent with remarkable cell growth inhibition activity against various cancer types, especially non-small lung (NSCLC), but its molecular mechanism still unclear. Our previous study found that miR-628-3p could inhibit the of A549 cells and induce apoptosis. Bioinformatics analysis predicted promoter sequence contained p53 binding sites. Considering regulatory effect SHK on p53, we speculate may apoptosis NSCLC by up-regulating miR-628-3p. CCK-8 EdU assay...
Lung cancer is one of the leading causes cancer-related death in world. MicroRNA- (miR-) 628-3p plays critical roles many cancers, including lung cancer. We investigated how miR-628-3p affected migration and apoptosis A549 cells. used bioinformatics algorithms to predict target gene study molecular mechanism by which contributes Then, we luciferase reporter assay identify whether heat shock protein 90a (HSP90) a direct miR-628-3p. Western blotting quantitative real-time PCR showed that...
Bletilla striata (Thunb.), an ornamental and medicinal plant, is on the list of endangered plants in China. Its pseudobulb abundant polysaccharide has been used for centuries as a herbal remedy. However, recent rise demand placed it at risk extinction, therefore, research its propagation genetic improvement essential. Since polyploids tend to possess advantageous qualities, we incubated B. seeds with colchicine aim creating tetraploid plantlets. Aseptic treated 0.1% 7 days showed highest...
Cytokine or growth factor activated STAT3 undergoes multiple post-translational modifications, dimerization and translocation into nuclei, where it binds to serum-inducible element (SIE, 'TTC(N3)GAA')-bearing promoters activate transcription. The DNA binding domain (DBD, 320–494) mutation in hyper immunoglobulin E syndrome (HIES), called the HIES (R382Q, R382W V463Δ), which elevates IgE synthesis, inhibits SIE activity sensitizes genes such as TNF-α for expression. However, mechanism by gene...
Systemic lupus erythematosus (SLE) is a chronic inflammatory disease mainly characterized by B cell hyperactivity. Glucocorticoid (GC) widely used in SLE for its potent anti-inflammatory and immunosuppressive effects. Despite important clinical efficacy, high-dose or long-term use of GC can cause severe side effects, such as osteoporosis, osteonecrosis, cataracts, hyperglycemia, coronary heart cognitive impairment. Our early studies have shown that Jieduquyuzishen prescription (JP)...
Atractylodes macrocephala Koidz. (AM) is a rhizome plant traditionally used as an herbal medicine to normalize gastrointestinal function and treat inflammation-related diseases. However, there are no comprehensive studies that have examined the chemical composition or biological activity of aerial parts AM, which may be promising raw material for pharmaceutical industry. The aim this study was investigate constituents, anti-oxidant, anti-inflammatory anti-septic activities essential oil...
Abstract LIF maintains self‐renewal growth in mouse embryonic stem cells (mESC) by activating STAT3, which translocates into nucleus for pluripotent gene induction. However, the ERK signaling pathway activated at large counteract with induction during growth. Here, it is reported that mESC STAT3 undergoes multivesicular endosomes (MVEs) translocation and subsequent secretion, LIF‐activated acetylated on K177/180 phosphorylated Y293 residues within N‐terminal coiled‐coil domain, responsible...
摘要:
Lupus nephritis (LN) is a common manifestation of Systemic lupus erythematosus (SLE), characterized by higher activity Signal Transducer and Activator Transcription 3 (STAT3) than in SLE patients without renal involvement. The relationship between estrogen receptorβ (ERβ) STAT3 the progression disease remains controversial. In this study, we found that LIF induced phosphorylation ERβ acetylation. These modifications mediated their interaction, then was enhanced ERβ. Genistein an isoflavonoid...
Background & AimsThe effect fraction of Bletilla striata (EFBS), a phenolic-rich extract, has significant protective effects on lipopolysaccharide (LPS)-induced acute lung injury (ALI), but its composition and molecular mechanisms are unclear. This study elucidated chemical possible against LPS-induced ALI from an antioxidant perspective.MethodsEFBS was prepared by ethanol extraction, enriched polyamide column chromatography, characterized using ultra-performance liquid...
With basal medium, we studied the growth status, lipid droplet distribution, total content of Chlorella protothecoides CS-41 treated with different concentrations sodium chloride (0, 150, 300 and 600 mmol/L) by optical microscopy, electron confocal laser focusing Nile red staining. Results show that addition NaCl affected CS-41. increase concentration, rate was inhibited. cell wall became thicker, droplets increased. At early stage, amount in mmol/L culture highest, but at late-log increased...