A5038217841- Probiotics and Fermented Foods
- Milk Quality and Mastitis in Dairy Cows
- Polysaccharides and Plant Cell Walls
- Plant-Microbe Interactions and Immunity
- Bacterial biofilms and quorum sensing
- Essential Oils and Antimicrobial Activity
- Microbial Metabolism and Applications
- Antimicrobial Resistance in Staphylococcus
- Antimicrobial Peptides and Activities
- Enzyme Production and Characterization
- Insect symbiosis and bacterial influences
- Gut microbiota and health
- Microbial infections and disease research
- Fungal Biology and Applications
- Microbial Metabolites in Food Biotechnology
- Rabies epidemiology and control
- Aquaculture disease management and microbiota
- Venomous Animal Envenomation and Studies
- Mycotoxins in Agriculture and Food
- Phytochemistry Medicinal Plant Applications
- Biofuel production and bioconversion
- Bacteriophages and microbial interactions
- Insect Resistance and Genetics
- Biochemical Analysis and Sensing Techniques
- Phytochemistry and biological activity of medicinal plants
Universidade Federal de Viçosa
2013-2024
Roslin Institute
2022
University of Edinburgh
2022
One manner in which plant-derived compounds exert their antibiotic potential is the synergism, a positive interaction between two compounds. Studies indicate that use of plant extracts combined with antimicrobials may promote significant reduction minimum inhibitory concentrations antibiotics for bacterial strains. This study aimed to evaluate activity and as well combination on Staphylococcus aureus. The 15 was evaluated using diffusion assay. (MICs) interactions compound emodin were...
Staphylococcus aureus is associated with chronic mastitis in cattle, and disease manifestation usually refractory to antibiotic therapy. Biofilm production a key element of S. pathogenesis may contribute the treatment failure that consistently reported by veterinarians. Minas Gerais State largest milk-producing state Brazil, characterization bacterial isolates an important aspect control for dairy farmers. Here, we investigated potential isolated from bovine produce slime biofilm skim-milk...
The characterization and identification of proteolytic bacteria from the gut velvetbean caterpillar (Anticarsia gemmatalis) were objectives this study. Twelve aerobic anaerobic isolates obtained in calcium caseinate agar. number colony forming units (CFUs) was higher when extracted caterpillars reared on artificial diet rather than soybean leaves (1.73 ± 0.35 × 103 0.55 0.22 CFU/mg gut, respectively). isolated divided into five distinct groups, according to their polymerase chain...
Quorum sensing is used by bacteria to coordinate gene expression in response population density and involves the production, detection extracellular signaling molecules known as autoinducers (AIs). Salmonella does not synthesize AI-1, acyl homoserine lactone (AHL) common gram-negative bacteria; however, it has a receptor for SdiA protein. The effect of modulating phenotypes been elucidated. In this report, we provide evidence that AIs-1 affect enterica serovar Enteritidis behavior enhancing...
Bovine mastitis is a major threat to animal health and the dairy industry. Staphylococcus aureus contagious pathogen that usually associated with persistent intramammary infections, biofilm formation relevant aspect of outcome these infections. Several biological activities have been described for snake venoms, which led us screen secretions Bothrops jararacussu antibiofilm activity against S. NRS155. Crude venom was fractionated by size-exclusion chromatography, fractions were tested...
Staphylococcus aureus is the main causative agent of bovine mastitis. The activity several extracts from ten medicinal plants traditionally used in Brazil as antiseptic was investigated against fifteen strains isolated animals with mastitis manifestation by disc diffusion method and broth microdilution assay. interference on cell form adherent colonies also evaluated. MIC values ranged 0.5 mg/mL to 1.0 biofilm inhibitory concentration (BIC) were between 0.25 0.8 mg/mL. Results revealed...
Many efforts have been made to understand the pathogenesis of bovine mastitis reduce losses and promote animal welfare. Staphylococcus aureus may cause clinical mastitis, but it is mainly associated with subclinical infection, which usually persistent can easily reoccur. Here, we conducted a comparative genomic analysis between strains S. causing infection (Sau170, 302, 1269, 1364), previously sequenced by our group, two well-characterized (N305 RF122) find differences that could be linked...
The advent of the first synthetic cell has triggered significant interest in biology research and its potential impact on various fields, including industry, agriculture, health, environment. With unique blend molecular biology, genetics, engineering, computational modelling, is becoming a crucial tool addressing challenges food science. For example, development genetic circuits underway for improvement safety quality, such as creating biosensors detecting toxins heavy metals, enhancing...
Individual traits vary among and within populations, the co-occurrence of different endosymbiont species a host may take place under varying loads in each individual host. This makes recognition potential impact such associations insect difficult, particularly pest species. The maize weevil, Sitophilus zeamais Motsch. (Coleoptera: Curculionidae), key stored cereal grains, exhibits with two endosymbiotic bacteria: obligatory SZPE ("Sitophilus Primary Endosymbiont") facultative Wolbachia. lack...
Resistance of pathogens to common veterinary antibiotics hampers mastitis treatment and motivates the discovery new antimicrobials. In this study, extracts from two aquatic plants, Salvinia auriculata Hydrocleys nymphoides, were assayed against bovine pathogens. Selected parts plants extracted with different solvents. The showed activity only Gram-positive strains tested largest inhibition zones seen for hexane extracts. minimum inhibitory concentration values ranged 0.2 1.0 mg/ml. Growth...
Kefir has been suggested as a possible bacterial prophylaxis against Salmonella and IL-10 production seems to be crucial in the pathogenesis of salmonellosis mice. This study evaluated role inflammation gut microbiome mice consuming milk kefir orally challenged with enterica serovar Typhimurium. C57BL wild type (WT) (n = 40) IL-10-/- (KO) were subdivided into eight experimental groups either treated or not kefir. In first 15 days, water received filtered (0.1 mL) while (10% w/v) by gavage....
Penicillium griseoroseum has been studied by our group because of its good pectinase production. Attempts have done to clone pectinolytic genes, aiming obtain pectinase-overproducing strains for industrial purposes. Here, two genes coding pectin lyase were isolated from the P. genome. The plg1 gene an open reading frame 1341 bp a putative protein 374 amino acids with calculated molecular mass 40.1 kDa. plg2 is characterized 1400 nucleotides and codes polypeptide 383 acids. 5′-flanking region...
Salmonella spp are among the main causative agents of foodborne diseases.Some phenotypes associated with increased drug resistance and virulence regulated by quorum sensing (QS).In present study, autoinducer (AI)-1-and -2-mediated QS mechanisms were characterized in enterica serovar Enteritidis PT4 for first time.Salmonella did not produce AI-1.Phylogenetic analysis nucleotides encoding SdiA protein, response regulator AI-1-mediated QS, comparative alignment its amino acids showed that gene...
Antibiotic resistance is a serious global threat to public health. This has promoted the research for new drug targets, and use of other approaches, such as antimicrobial combined therapy. The present study evaluated antibacterial activity 88 extracts from Brazilian Atlantic Forest trees. organic extract leaves Miconia latecrenata (EMl) was most promising inhibiting growth Staphylococcus aureus (0.3 mg/mL) Pseudomonas aeruginosa (2.5 mg/mL). After bioguided fractionation EMl metabolite...
This work describes a green synthesis, the characterization, and biological evaluation of silver nanoparticles (AgNPs). The AgNPs suspension was synthesized using aqueous leaf extract Eucalyptus grandis, which presented characteristic band at 407 nm in UV-Vis spectrum. spherical shape size 9.7 ± 0.3 nm. were stable over month, indicating that E. grandis’ is suitable for their preparation stabilization. X-ray analysis showed crystallinity corresponded to centered face phase silver....