A5073437682- Milk Quality and Mastitis in Dairy Cows
- Probiotics and Fermented Foods
- Microbial infections and disease research
- Genetic and phenotypic traits in livestock
- Microbial Metabolism and Applications
- Digestive system and related health
- Identification and Quantification in Food
- Biopolymer Synthesis and Applications
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Enterobacteriaceae and Cronobacter Research
- Animal Genetics and Reproduction
- Ruminant Nutrition and Digestive Physiology
- Genomics and Phylogenetic Studies
- Mycobacterium research and diagnosis
- Bacterial biofilms and quorum sensing
- Food Safety and Hygiene
- Meat and Animal Product Quality
- Crop Yield and Soil Fertility
- Diphtheria, Corynebacterium, and Tetanus
- Effects of Environmental Stressors on Livestock
- Biosensors and Analytical Detection
- Essential Oils and Antimicrobial Activity
- Veterinary Oncology Research
- Animal Behavior and Welfare Studies
Michigan State University
2021-2025
Universidade Estadual de Campinas (UNICAMP)
2023-2024
Universidade de São Paulo
2012-2023
Military Institute of Engineering
2023
Medica (United States)
2021
Brazilian Buiatrics Association
2021
Clínica MEDS (Chile)
2021
Técnicas Reunidas (Spain)
2021
North Carolina State University
2017-2019
Hospital Universitário da Universidade de São Paulo
2014
Coagulase-negative staphylococci (CoNS) are among the main pathogens causing bovine intramammary infection (IMI) in many countries. However, one of limitations related to specific diagnosis CoNS is lack an accurate, rapid, and convenient method that can differentiate bacterial species comprising this group. The aim study was evaluate ability matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) accurately identify dairy cow IMI. In addition, aimed determine...
Most new pathogens of humans and animals arise via switching events from distinct host species. However, our understanding the evolutionary ecological drivers successful adaptation, expansion, dissemination are limited. Staphylococcus aureus is a major bacterial pathogen leading cause mastitis in dairy cows worldwide. Here we trace history bovine S. using global dataset 10,254 genomes including 1,896 isolates 32 countries 6 continents. We identified 7 contemporary endemic clones causing...
Abstract We evaluated the effects of chronic subclinical mastitis (CSM) caused by different types pathogens on milk yield and components at cow level. A total 388 Holstein cows had measured were sampled three times intervals two weeks for determination SCC composition, microbiological culture was performed. Cows considered healthy if all samples ≤200 000 cells/ml culture-negative third sampling. with one result > 200 to suffer non-chronic whereas least 2 out 3 results CSM. These latter...
This study aimed to assess (a) the biofilm producer ability and antimicrobial resistance profiles of Staphylococcus (Staph.) aureus Streptococcus (Strep.) uberis isolated from cows with clinical mastitis (CM) subclinical (SCM), (b) association between resistance. We a total 197 Staph. strains (SCM = 111, CM 86) 119 Strep. 15, 104) milk samples obtained 316 distributed in 24 dairy herds. Biofilm-forming was assessed using microplate method, while susceptibility determined disk diffusion...
Staphylococcus aureus is a major cause of subclinical mastitis in dairy cows, and its development antibiotic resistance has limited treatment efficacy. Essential oils (EOs) are natural products with wide range antimicrobial properties that could be used to treat bovine mastitis. This study aims investigate the activity EOs against S. isolated from cases State São Paulo—Brazil. A total 14 isolates were selected, based on presence biofilm-forming genes (icaA, icaD, bap), cultured final...
The objective of this study was to compare the minimum inhibitory concentrations antimicrobials included in a commercial broth microdilution panel among Gram-positive pathogens that caused non-severe clinical mastitis on three Michigan dairy farms. Duplicate quarter milk samples were collected from eligible quarters cows enrolled randomized trial, cultured university laboratory, and identified using MALDI-TOF. Etiologies grouped by genus as Enterococcus species (n = 11), Lactococcus 44),...
Mastitis, an inflammatory condition of the udder, can be caused by entry Staphylococcus aureus, whose adhesion to mammary epithelial cells is influenced virulence factors such as microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) and accessory gene regulator (agr) system. Our goal was determine invasion rates S. aureus isolates from clinical (mild moderate) subclinical mastitis assess impact MSCRAMM genes agr types on disease severity. Clinical predominantly...
The aim of this research was to describe the incidence and treatments mastitis other common bovine diseases using one year retrospective observational data (n = 50,329 cow-lactations) obtained from herd management software 37 large dairy farms in Wisconsin. Incidence rate (IR) defined as number first cases each disease divided by lactations per farm. Clinical (CM) remains most diagnosed cows. Across all herds, mean IR (cases 100 24.4 for clinical mastitis, 14.5 foot disorders (FD), 11.2...
This research study aimed to evaluate the use of milk leukocyte differential (MLD) to: (a) identify quarter milks that are culture-positive; and (b) characterize responses specific groups pathogens causing subclinical mastitis. The MLD measures absolute number relative percentage inflammatory cells in samples. Using two dairy herds (170 172 lactating cows, respectively), we studied all cows with a most recent monthly Dairy Herd Improvement Association somatic cell count (SCC) >200 × 10 3...
The core objective of this study was to genetically and phenotypically characterize subclinical mastitis-causing multidrug-resistant Staphylococcus aureus (MDRSA). In addition, risk factors associated with mastitis caused by MDRSA were investigated. Bacterial cultures performed on 2120 mammary quarters, 40 swabs milk utensils, 5 bulk tank samples, 11 nostril hand from milkers five dairy farms. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)...