A5049006856- Pesticide Residue Analysis and Safety
- Helminth infection and control
- Bacterial Identification and Susceptibility Testing
- Biochemical Analysis and Sensing Techniques
- Advanced Chemical Sensor Technologies
- Milk Quality and Mastitis in Dairy Cows
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotics Pharmacokinetics and Efficacy
- Microbial infections and disease research
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Genomics and Phylogenetic Studies
- Analytical Chemistry and Chromatography
- Enterobacteriaceae and Cronobacter Research
- Mass Spectrometry Techniques and Applications
- Potato Plant Research
- Plant Diversity and Evolution
- Diphtheria, Corynebacterium, and Tetanus
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Mycotoxins in Agriculture and Food
- Plant Pathogens and Resistance
- Molecular Biology Techniques and Applications
- Dye analysis and toxicity
- Phytochemical compounds biological activities
Universidade Estadual de Campinas (UNICAMP)
2015-2025
Universidade de São Paulo
2012
Universidade Federal de São Carlos
2004-2012
Coagulase-negative staphylococci (CoNS) are among the main pathogens causing bovine intramammary infection (IMI) in many countries. However, one of limitations related to specific diagnosis CoNS is lack an accurate, rapid, and convenient method that can differentiate bacterial species comprising this group. The aim study was evaluate ability matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) accurately identify dairy cow IMI. In addition, aimed determine...
The presence of ochratoxin A (OTA) in wine is mainly due to the contamination grapes by Aspergillus carbonarius and A. niger, still vineyard or at stages prior winemaking. Throughout winemaking process, although there a reduction OTA levels, modified mycotoxins may also be formed. In fact, are compounds that normally remain undetectable during conventional analysis used for parent toxin. this context, current study aimed evaluate effect grape variety steps on fate as well formation...
For more than a century, bacteria and fungi have been identified by isolation in culture followed enzymatic reactions morphological analyses. The identification of environmental microorganisms, however, remains challenge because biochemical staining protocols for are tedious, usually stepwise, can be long (days) prone to errors. Molecular techniques based on DNA amplification and/or sequencing provide secure molecular specific bacteria, but mass spectrometry (MS), mainly MALDI-MS, has shown...
The antioxidant and anti-inflammatory effects of the aqueous extract Passiflora edulis leaves, which is a source vitexin, isovitexin isoorientin, was investigated in 2,4,6-trinitrobenzenesulphonic acid induced colitis model. oral intake P. (1100 µg mL−1) improved significantly (P < 0.05) endogenous status decreased lipid peroxidation serum, liver, colon. consumption level pro-inflammatory colon tissue, especially by reducing IL-1β five-fold TNF-α two-fold compared to control group. leaves...
With the increasing demand for reduced sugar or sugar-free products by consumers with chronic non-communicable diseases and individuals seeking weight control, foods beverages high-intensity sweeteners (HIS) tend to become more common in market. Therefore, monitoring HIS concentrations added industrialised is an important issue. Thus, analytical method using LC-MS/MS was established validated order allow simultaneous determination of nine these compounds marketed Brazil. levels were...
Traditional methods for bacterial identification include Gram staining, culturing, and biochemical assays phenotypic characterization of the causative organism. These can be time-consuming because they require in vitro cultivation microorganisms. Recently, however, it has become possible to obtain chemical profiles lipids, peptides, proteins that are present an intact organism, particularly now new developments have been made efficient ionization biomolecules. MS therefore state-of-the-art...
Government regulatory actions and public policies have been recently implemented in Brazil due to the excessive consumption of sugar. Therefore, it becomes relevant determine levels high-intensity sweeteners tabletop consumed by Brazilian population. Thus, an analytical method was developed validated for simultaneous determination nine (acesulfame potassium, aspartame, advantame, sodium cyclamate, neotame, saccharin, sucralose, stevioside, rebaudioside A) using ultra-high performance liquid...
Trattinnickia burserifolia has yielded the known ursanes, alpha-amyrenone, alpha-amyrin, 3-epi-alpha-amyrin, 3alpha,16beta-dihydroxyurs-12-ene, oleananes beta-amyrenone, beta-amyrin, 3-epi-beta-amyrin, 3alpha,16beta-dihydroxyolean-12-ene, tirucallane acids 3alpha-hydroxytirucall-8,24-dien-21-oic, 3alpha-hydroxytirucall-7,24-dien-21-oic and 3-oxotirucall-8,24-dien-21-oic, dammaranes dammarenediol-II 3alpha,20(S)-dihydroxydammar-24-ene. Besides it was isolated new monoterpene...
Commercial cattle breeders produce their own herd offspring for the dairy and beef market using artificial insemination. The procedure involves sanitary risks associated with collection commercialization of germplasm, in vitro production transfer bovine embryos must be monitored by strict health surveillance. To avoid spreading infectious diseases, one rely on controlled media, reagents that are guaranteed free pathogens. In this article, we investigated use a new mass spectrometric approach...
ABSTRACT: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been shown to be an alternative method for identification of bacteria via their protein profile spectra, being able identify at the genus, species and even subspecies level. With aim large-scale pathogens causing mastitis by this platform, a total 305 isolates identified from cows with subclinical were analyzed conventional microbiological culture (MC) as well MALDI-TOF MS coupled...
Focusing on the demand from food industry for fast and reliable alternative methods to control quality of products, we present in this paper a method amino acid separation glutamic quantification complex matrices employing capillary electrophoresis with capacitively coupled contactless conductivity detection. We demonstrate by simulation experimentally use organic solvents sample preparation prevent peak splitting increase stacking electrophoretic separations acids. Additionally, obtained...
Bakery products, including biscuits, cakes and breads, generally present a high content of simple sugars rapid absorption, fat low amount dietary fiber, which make them highly caloric foods. Although sucrose is very important ingredient in bakery products for its preservation characteristics significant source energy, there growing interest replacing this sugar with alternative substances, such as high-intensity sweeteners (HIS) that provide sweetness no or calories. In Brazil, data on the...
The methanol extract from the stems and fruits of Swinglea glutinosa (Rutaceae) afforded 11 known acridone alkaloids three N-phenylethyl-benzamide derivatives, glycocitrine-IV, 1,3,5-trihydroxy-4-methoxy-10-methyl-2,8-bis(3-methylbut-2-enyl)acridin-9(10H)-one, 1,3,5- trihydroxy-2,8-bis(3-methylbut-2-enyl)-10-methyl-9-acridone, citbrasine, citrusinine-II, citrusinine-I, 5-dihydroxyacronycine, pyranofoline,...
The known anti-protozoal activity of flavonoids has stimulated the testing other derivatives from natural and synthetic sources.As part our efforts to find potential lead compounds, a number isolated Neoraputia paraensis, N. magnifica, Murraya paniculata, (Rutaceae), Lonchocarpus montanus, L. latifolius, subglaucescens, atropurpureus, campestris, Deguelia hatschbachii (Leguminosae), dibenzoylmethanes subglaucescens analogues were tested for in-vitro against chloroquine-sensitive Plasmodium...