Plastic microfluidic device for the efficient isolation (>90% recovery) of cell free DNA from plasma molecular profiling.

We present a portable, battery-operated and application-specific lab-on-a-chip (ASLOC) system that can be easily configured for wide range of applications. It is based on multiplexed electrical current detection serves as the sensing system. demonstrate different configurations to perform most schemes currently in use LOC systems, including some advanced such nanowire-based biosensing, surface plasmon resonance sensing, electrochemical real-time PCR. The complete controlled by single chip...

Concern about the environment is increasing and so search for analytical methods that make continuous monitoring possible. Microfluidic devices such as lab-on-a-chip emerge an alternative to laboratory-based conventional techniques, making possible development of unmanned tools. This review covers last five years on application autonomous microfluidic environmental addresses existing demands in this field.

The direct coupling of free-flow isotachophoresis device with an electrospray-ionization-mass-spectrometer allows continuous online detection without any sample preparation such as purification and labeling.

Through simulations and experiments, we analyzed the effects of electrode configuration applied voltage in heating, mixing, particle enrichment by AC electrothermal effects. We focused on spacing between groups electrodes finger within groups. Our findings revealed that having tight led to more even heating better mixing. also highlighted role distance enhancing contributed valuable insights for optimizing design microfluidic systems.

The objective of this work was to study the effect blanching and influence temperature, solution concentration, initial fruit:solution ratio on osmotic dehydration star-fruit slices. For blanching, different concentrations citric ascorbic acids were studied. samples immersed in 0.75% acid presented little variation color relation fresh star-fruit. Osmotic carried out an incubator with orbital shaking, controlled constant shaking at 120 rpm. process variables studied trials defined by a...

The polyhedron which confines the Poynting vectors of N-beam transmissions in crystal space is referred to as Borrmann pyramid. observation this pyramid realized from diffraction images four-beam, (000)(220)(2\bar 20)(400), transmission case silicon single crystals for Mo Kα. directions eight modes wave propagation involved and are calculated. These calculations confirm experimental observations. variation direction vector each mode also reported.

Focusing on the demand from food industry for fast and reliable alternative methods to control quality of products, we present in this paper a method amino acid separation glutamic quantification complex matrices employing capillary electrophoresis with capacitively coupled contactless conductivity detection. We demonstrate by simulation experimentally use organic solvents sample preparation prevent peak splitting increase stacking electrophoretic separations acids. Additionally, obtained...

We present a method of continuous electroextraction amino acids using aqueous two phase system in microchip. The separations occur due to differences electrophoretic mobility and solvent affinity. results suggest the possibility high levels purification by controlling electric field across liquid barrier.

The application limit of the MULTX program for predicting Renninger-scanning X-ray multiple diffraction data is extended in order to simulate Renninger scans semiconductor single-crystal and heteroepitaxial structures recorded using synchrotron radiation. experimental synchrotron-radiation scan InP(006) bulk material taken as standard analyse effects both polarization factor diffracted-beam path length. beam considered a matrix approach. length involved surface secondary cases analysed...

We present an on-line, single step coupling between liquid-liquid extraction and capillary electrophoresis with capacitively coupled contactless conductivity detection, which allows efficient analysis of complex food matrices high sodium content. The depletion was demonstrated using aqueous two-phase system. system enables the electrically driven target compounds. sample prepared in Dextran-rich phase (8% w/v 500 kDa Dextran, DEX). background electrolyte (acetic acid 5.0 mol/L) contained 6%...

The influence of a magnetic field on electrodeposition nickel films over polymeric substrates, which renders flexible membranes with permanent magnet layer, has been studied. Samples deposited by electroplating in presence and absence are compared. electroplated layer is an electroless contact after wet pre-treatment to improve adherence. Mechanical properties the samples were measured as function process parameters. Experimental results confirm that positive impact films. Pre-treatment...

TRENDS.Sample preparation is commonly considered a key step to achieve selective, sensitive, and reliable chemical analyses, particularly those involving complex matrices.Although the application of electric fields improve speed efficiency sample methods has been proven, this approach still be state-of-the-art; hence, further development necessary future applications.This review describes fundamentals, advances, applications, perspectives using enhance techniques such as liquid-liquid...

Liquid biopsy markers, which can be secured from a simple blood draw or other biological samples, are used to manage variety of diseases and even monitor for bacterial viral infections. Although there several different types liquid the subcellular ones, including cell-free DNA, microRNA, extracellular vesicles, particles, evolving in terms their utility. A challenge with markers is that they must enriched sample prior analysis because vast minority mixed population, potential interferences...

An experimental facility for carrying out x-ray multiple diffraction (XRMD) studies in parallel-beam geometry using the Daresbury synchrotron radiation source and its application study of coherency an epilayered sample are described. Experimental high-resolution Renninger scans (RS) about GaAs(006) presented pseudoforbidden ‘‘Aufhellung’’ eight-beam (000,006,020,042,044,026,02̄4,02̄2) case has been fully resolved first time setup which involves a double-crystal six-circle scattering data...

In this paper, we further investigate the properties of off-stoichiometry thiol-ene polymers (OSTE) aiming its application in microchip electrophoresis for bioanalytical applications. The proportion 1.3:1 (allyl:thiol) and 1:2.5 presented best results terms sealing. Raman imaging mapping surfaces revealed an outstanding homogeneity. Water contact angle were measured as 68° ± 6° 71° 5° allyl thiol, respectively. Substrates prepared with OSTE demonstrated to be less prone sorption nonpolar...

This work presents a broadband setup for single-cell electrorotation. It is capable of generating relatively high voltage in continuous frequency band starting from 50 kHz up to 5 GHz. The shows very small phase and amplitude imbalance, which were found negligible as no decentric movement the cell was observed. At least 9.6 V peak-peak at single electrode achieved with total harmonic distortion less than 5%. results obtained on lymphocytes follow theoretical curves, show that higher...

Abstract This study addresses the variations observed in electrorotation measurements due to cell positioning and movement. Electrorotation provides a non-disruptive method for inferring electrical properties of individual cells. However, its widespread adoption is hindered by significant variation speed. By mitigating impact positional dependencies other influencing factors, our methodology opens avenues broader applications single-cell analysis without need complex setups trap retain...

DOI: 10.1002/elps.201800203 An on-line, single step electroextraction is demonstrated for capillary electrophoresis and microchip electrophoresis. The employed an aqueous two-phase system (ATPS) formed by one 500 kDa dextran-rich phase added to the samples a second 6 poly(ethylene glycol)-rich containing 5.0 mol/L acetic acid. acted as background electrolyte. ATPS enabled electrically driven extraction of target compounds, while retain sodium ions. electrodriven was applied on determination...