We studied the beta-lactamases of an E. aerogenes isolate recovered from blood a two-year-old patient. The demonstrated disk-diffusion phenotype typical for AmpC-ESBL co-producer. Microbiology studies were performed according to standard protocols. resistance gene was identified by transconjugation and cloning experiments. By only narrow spectrum beta-lactamase (TEM-1) encoded on small plasmid transmitted. ESBL cloned expressed in coli host. Sequence analysis recombinant revealed blaSHV-12...

The level of heterogeneity among the isolated stem cells makes them less valuable for clinical use. purpose this study was to understand human dental pulp derived mesenchymal by using basic cell biology and proteomic approaches. were from a natal (NDPSCs), an exfoliated deciduous (stem (SHED)), impacted third molar (DPSCs) tooth three different donors. All displayed similar features related morphology, proliferation rates, expression various surface markers, differentiation potentials into...

ABSTRACT Thymoquinone (TQ) has shown antitumorigenic effects in breast cancer; however, its detailed impact on cell signaling mechanisms requires further investigation. This study aims to elucidate the molecular behind TQ's antiproliferative cancer by analyzing proteome‐level changes. MCF‐7 cells were treated with 15 µM TQ, inhibitory concentration (IC50), for 48 h. Proteins from and untreated (control) groups isolated subjected liquid chromatography–tandem mass spectrometry (LC–MS/MS)...

Background/Aim: Prostate cancer is the second leading cause of cancer-related deaths in men. Periodontitis considered a high-risk factor for prostate cancer, but genetic mechanism unclear. This study aims to identify dysregulated miRNAs, their associated genes, signaling pathways, and compounds linking periodontitis cancer. Materials Methods: The miRNA expression datasets were obtained from GEO database. Differentially expressed miRNAs (DEmiRNAs) identified, common DEmiRNAs (Co-DEmiRNAs)...

Introduction: The success of emergency cerclage in the presence incompetent cervix and protruding fetal membrane is a matter debate. Theories suggest that microbiome may play an important role influencing structural integrity cervix. Hence, understanding status before operation have value predicting rate procedure. This study aimed to examine existence microbial organisms amniotic fluid through polymerase chain reaction (PCR) detection 16S rRNA species assess its correlation with perinatal...

The use of Lucilia sericata larvae on the healing wounds in diabetics has been reported. However, role excretion/secretion (ES) products treatment diabetic remains unknown. This study investigated whether application ES L. wound surface could improve impaired streptozotocin-induced rats. Additional analysis was performed to understand proteome content secretome contribution at molecular level. For this purpose, full-thickness skin were created backs and control A conducted assess levels...

Small cell lung carcinoma (SCLC) is a highly aggressive cancer with low survival rate. Although initial response to chemotherapy in SCLC patients well-rated, the treatments applied after disease relapses are not successful. Drug resistance accepted be one of main reasons for this failure. Therefore, there an urgent need new treatment strategies SCLC. Meclofenamic acid, nonsteroidal anti-inflammatory drug, has been shown have anticancer effects on various types cancers via different...

Abstract Placenta accreta spectrum (PAS) presents a significant obstetric challenge, associated with considerable maternal and fetal-neonatal morbidity mortality. Nevertheless, it is imperative to acknowledge that noteworthy subset of PAS cases remains undetected until the time delivery, thereby contributing an augmented incidence among affected individuals. The delayed identification not only hinders timely intervention but also exacerbates health risks for both fetal outcomes. This...

It is well-known that diverse types of blood proteins contribute to healing process via different mechanisms. Presence and potential involvements blood-derived abundant in the platelet-rich fibrin (PRF) its regenerative capacity have not been sufficiently emphasized literature. The aim this paper was analyze proteome content PRF summarize previously reported effects identified on wound a literature review.The samples obtained from non-smoking, systemically healthy volunteers were subjected...

Context: Isolation and characterization of OXA-162, a novel variant OXA-48.Objectives: Klebsiella pneumoniae isolate with decreased susceptibility to carbapenems was recovered from Turkish patient. This study aimed at characterizing the carbapenem resistance determinants this isolate.Materials methods: Antibiotic tests, analytic isoelectric focusing (IEF), cloning sequencing were performed. Cloned β-lactamase purified by means preparative gel electrophoresis kinetic constants determined...

ABSTRACT In this study, we aimed to investigate the potential use of keratin biofiber (KBF)‐reinforced biodegradable thermoplastic polyurethane (TPU) composites as tissue engineering scaffolds. The were prepared by a solution casting method. Combined salt leaching and thermally induced phase‐separation methods used manufacture porous scaffold structures. mechanical properties investigated with tensile tests dynamic analysis (DMA). An increase in fiber content increased modulus strength...

Background/Aim: Breast cancer (BC) incidence and mortality rates have been increasing due to the lack of appropriate diagnostic tools for early detection. Proteomics-based studies may provide novel targets diagnosis efficient treatment. The aim this study was investigate global changes occurring in protein profiles breast tissues discover potential or prognostic biomarkers. Materials Methods: BC their corresponding healthy counterparts were collected, subtyped, subjected comparative...

An intensive care unit (ICU)-based OXA-23-producing multiple-drug resistant Acinetobacter baumannii (MDRAB) outbreak was detected between October 2005 and 2006. A total of 47 patients were infected/colonized with the strain. Clinical data available from 37 patients. The all-cause mortality rate among exposed to epidemic strain 35% (13/37). resistance determinants characterized both by microbiological methods molecular techniques. Cloning sequencing experiments identified ISAbaI-associated...