Constantly expressed genes are used as internal controls in relative quantification studies. Suitable for such studies have not yet been defined Pseudomonas aeruginosa. In this study, the ampC, fabD, proC, pbp-2, rpoD and rpoS of P. aeruginosa were compared terms expression stability by real-time quantitative RT-PCR. A total 23 strains with diverse resistance phenotypes studied. Stability among housekeeping was assessed on basis correlation coefficients, best-correlated pair accepted being...

We studied the beta-lactamases of an E. aerogenes isolate recovered from blood a two-year-old patient. The demonstrated disk-diffusion phenotype typical for AmpC-ESBL co-producer. Microbiology studies were performed according to standard protocols. resistance gene was identified by transconjugation and cloning experiments. By only narrow spectrum beta-lactamase (TEM-1) encoded on small plasmid transmitted. ESBL cloned expressed in coli host. Sequence analysis recombinant revealed blaSHV-12...

PER-1 type beta-lactamases were screened among ceftazidime-resistant clinical isolates of Acinetobacter spp. and Pseudomonas aeruginosa. A total 176 non-repetitive (84 92 P. aeruginosa) collected during a three month surveillance period. Isolates obtained from seven intensive care units university hospitals. All strains for bla(PER-1) alleles by PCR. Of the strains, 31% 55.4% aeruginosa positive genes, respectively.

In this study, the mechanical, antibacterial properties and cell toxicity response of Ti-5Al2.5Fe alloy with different copper contents were investigated. The alloys prepared by high-energy ball milling using elemental Ti, Al, Fe, Cu powders consolidated a uniaxial vacuum hot press. Staphylococcus aureus strain ATCC 29213 Escherichia coli 25922 used to determine sintered alloys. in vitro cytotoxicity samples was evaluated HeLa (ATTC, CCL-2) cells thiazolyl blue tetrazolium bromide. mechanical...

Context: Isolation and characterization of OXA-162, a novel variant OXA-48.Objectives: Klebsiella pneumoniae isolate with decreased susceptibility to carbapenems was recovered from Turkish patient. This study aimed at characterizing the carbapenem resistance determinants this isolate.Materials methods: Antibiotic tests, analytic isoelectric focusing (IEF), cloning sequencing were performed. Cloned β-lactamase purified by means preparative gel electrophoresis kinetic constants determined...

Introduction: Oral diseases affect billions worldwide, with dental caries being a significant concern. Silver-copper nanoparticles (Ag-Cu NPs) synthesized from pitaya plant extract offer potential solution due to their antimicrobial properties. This study aimed evaluate the inhibitory effects of Ag-Cu NPs on Streptococcus mutans (S. mutans), major contributor caries. Methodology: were using green chemical method and characterized scanning electron microscope (SEM), ultraviolet-visible...

A VIM-5-producing Enterobacter cloacae isolate (EDV/1) was identified in a collection of clinical strains stored before 2002. The gene, bla(VIM-5), located on 2,712-bp BamHI-HindIII fragment 23-kbp (approximately) nonconjugative plasmid (pEDV5) class 1 integron as single gene cassette.

An intensive care unit (ICU)-based OXA-23-producing multiple-drug resistant Acinetobacter baumannii (MDRAB) outbreak was detected between October 2005 and 2006. A total of 47 patients were infected/colonized with the strain. Clinical data available from 37 patients. The all-cause mortality rate among exposed to epidemic strain 35% (13/37). resistance determinants characterized both by microbiological methods molecular techniques. Cloning sequencing experiments identified ISAbaI-associated...

Immediately after the devastating earthquake in Turkey August 1999, an infectious disease surveillance system was established Kocaeli Province (the biggest area affected). This study mainly focused on diarrheal diseases. During a 33-day period, 1,468 stool cultures were processed. Diarrheal diseases increased step-by-step and later decreased to initial level by end of this period. Cases scattered throughout entire region, identified causes various, indicating multifocal increase. Of causes,...

The effects of imipenem and meropenem on the transcriptional expression resistance-related genes oprD, oprM oprN in Pseudomonas aeruginosa were studied by quantitative real-time PCR. Four strains examined: type strain PT5 (PAO1), its derivatives M7 PT149, a clinical isolate, PaKT3. derivative is nalB mutant, overexpressing MexAB-OprM pump, PT149 nfxC-type MexEF-OprN pump while it down-regulated for OprD protein. After 18 h incubation broth, cultures divided into three portions. Two...

In this study, pure polyurethane (PU) and poly (lactic) acid-silver nanoparticle (PLA-Ag NP) bicomponent nanofibers were successfully obtained alternately by using in the core or shell with coaxial electrospinning. The effects of displacements fibers on characterization, antibacterial, cytotoxicity properties whole examined detail. Using PLA-Ag NPs core, produced mats that thinner whereas locating to shell, more hydrophobic. TEM FTIR analysis proved PU are as a structure without mixing each...

Abstract It has been proposed that the virulence of nosocomial Staphylococcus infections associated with indwelling medical devices is related to ability bacterium colonise these materials by forming a biofilm composed multilayered cell clusters embedded in slime matrix. However, pathogenic role exopolysaccharide biofilms not fully understood. A new method was sought for differentiating structure from two closely bacterial strains, aureus and epidermidis. Using PCR it confirmed strains were...

The purpose of this study was to investigate the antibacterial activity various adhesive materials against five different oral streptococci. systems evaluated using agar diffusion tests. In each section plate, 6-mm-diameter wells were created with sterilized glass cylinders. Ten microlitres self-etch adhesives and control applied into shallow holes. After incubation at 37 °C for 24 h, growth inhibition zones measured in millimetres. Statistical analyses performed by two-way analysis variance...

To characterize common beta-lactamase genes and the role of integrons in beta-lactam antibiotic resistance among multidrug-resistant (MDR) P. aeruginosa strains isolated from intensive care units Turkey. Materials methods: After examination 67 isolates, 14 were found to be resistant all tested beta-lactams aminoglycosides. These studied beta-lactamases related integrons. Evaluation minimum inhibitory concentrations, polymerase chain reaction (PCR) screening, isoelectric focusing, outer...