A5053849487- Acute Lymphoblastic Leukemia research
- Cancer-related gene regulation
- Chronic Myeloid Leukemia Treatments
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Genomics and Rare Diseases
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Cancer Genomics and Diagnostics
- Viral gastroenteritis research and epidemiology
- BRCA gene mutations in cancer
- Platelet Disorders and Treatments
- Pharmacogenetics and Drug Metabolism
- Genetic factors in colorectal cancer
- T-cell and B-cell Immunology
- Lymphoma Diagnosis and Treatment
- Glutathione Transferases and Polymorphisms
- Iron Metabolism and Disorders
- Genomic variations and chromosomal abnormalities
- Congenital heart defects research
Kent Hastanesi
2019-2025
Acıbadem Adana Hospital
2019-2025
Acıbadem University
2020-2024
University of Applied Sciences Utrecht
2024
Istanbul University
2013-2023
HUN-REN Institute of Experimental Medicine
2022
WNT signaling has been implicated in the regulation of hematopoietic stem cells and plays an important role during T-cell development thymus. Here we investigated pathway activation childhood acute lymphoblastic leukemia (T-ALL) patients. To evaluate potential leukomogenesis, performed expression analysis key components pathway. More than 85% T-ALL patients showed upregulated β-catenin at protein level compared with normal human thymocytes. The impact this upregulation was reflected high...
Acute leukemias (ALs) are heterogeneous diseases. Functional polymorphisms in the genes encoding detoxification enzymes cause inter-individual differences, which contribute to leukemia susceptibility. The CYP2D6, CYP1A1, CYP2E1, GSTT1, and GSTM1 ALL (n = 156) AML 94) patients 140 healthy controls were genotyped by PCR and/or PCR-RFLP using blood or bone marrow samples. No association was observed between GSTT1 gene deletion (OR 0.8, 95% CI 0.4–1.7 for AMLs OR 0.9, 0.5–1.6 ALLs). Patients...
Abstract Objective Turkish genome is underrepresented in large genomic databases. This study aims to evaluate the effect of allele frequency population determining clinical utility germline findings breast cancer, including invasive lobular carcinoma (ILC), mixed ductal and (IDC‐L), (DC). Methods Two clinic‐based cohorts from Umraniye Research Training Hospital (URTH) were used this study: a cohort consisting 132 women with cancer non‐cancer 492 participants. The evaluation landscape was...
Abstract SOCS‐1, an important protein in the JAK/STAT pathway, has a role down stream of BCR‐ABL kinase. We investigated 56 CML patients and 16 controls for methylation status SOCS‐1 gene promoter Exon 2 regions. was found to be methylated 58.9% 93.8% [ P = 0.020, OR 0.121(0.015–0.957)%95CI]. The region unmethylated all patient samples controls. Although previous studies revealed relation between SOCS1 Exon‐2 hypermethylation development or progression, results this study showed no such...
The NOTCH signaling pathway plays important role in the development of multicellular organisms, as it regulates cell proliferation, survival, and differentiation. In adults, is essential for T- or B-lymphocyte lineage commitment. NOTCH1 FBXW7 mutations both lead activation are found majority T-ALL patients. this study, mutation analysis genes was performed 87 pediatric T-ALLs who were treated on ALL-BFM protocols. 19 patients (22%), activating observed either heterodimerization domain PEST 7...
Abstract Severe combined immunodeficiency (SCID) has a diverse genetic aetiology, where clinical phenotype, caused by single and/or multiple gene variants, can give rise to presentations. The advent of next‐generation sequencing (NGS) recently enabled rapid identification the molecular aetiology SCID, which is crucial for prognosis and treatment strategies. We sought identify various phenotypes SCIDs assessed both immunologic characteristics associated with variants. An amplicon‐based...
Abstract COVID-19 outbreak caused by SARS-CoV-2 created an unprecedented health crisis since there is no vaccine for this novel virus. Therefore, vaccines have become crucial reducing morbidity and mortality. In study, in vitro vivo safety efficacy analyzes of lyophilized candidates inactivated gamma-irradiation were performed. The candidate study OZG-3861 version 1 (V1), virus vaccine, SK-01 a GM-CSF adjuvant added vaccine. applied intradermally to BALB/c mice assess toxicity...
Rare disease patients constitute a significant part of the healthcare system all countries. However, information on experiences during processes and daily life rare is still limited. So far, there small number studies conducted in Türkiye, they mainly cover specific issues like education or anxiety. Here we present comprehensive survey analysis among their families within scope Istanbul Solution Platform for Undiagnosed Diseases-ISTisNA project. A total 498 individuals responded to survey,...
The NOTCH signaling pathway plays important role in the development of multicellular organisms, as it regulates cell proliferation, survival, and differentiation. In adults, is essential for T- or B-lymphocyte lineage commitment. NOTCH1 FBXW7 mutations both lead activation are found majority T-ALL patients. this study, mutation analysis genes was performed 87 pediatric T-ALLs who were treated on ALL-BFM protocols. 19 patients (22%), activating observed either heterodimerization domain PEST 7...
The effects of imipenem and meropenem on the transcriptional expression resistance-related genes oprD, oprM oprN in Pseudomonas aeruginosa were studied by quantitative real-time PCR. Four strains examined: type strain PT5 (PAO1), its derivatives M7 PT149, a clinical isolate, PaKT3. derivative is nalB mutant, overexpressing MexAB-OprM pump, PT149 nfxC-type MexEF-OprN pump while it down-regulated for OprD protein. After 18 h incubation broth, cultures divided into three portions. Two...
The renin–angiotensin system (RAS) is involved in cell growth, proliferation and differentiation bone marrow an autocrine–paracrine manner, it modulates normal neoplastic haematopoietic proliferation. This study aimed to assess expressions of the RAS components, renin, angiotensinogen angiotensin-converting enzyme (ACE), during imatinib mesylate treatment patients with chronic myeloid leukaemia (CML). Expressions components were studied CML at time diagnosis ( n = 83) 3, 6 12 months after...
Abstract The SARS-CoV-2 virus caused the most severe pandemic around world, and vaccine development for urgent use became a crucial issue. Inactivated formulated vaccines such as Hepatitis A smallpox proved to be reliable approaches immunization prolonged periods. In this study, gamma-irradiated inactivated does not require an extra purification process, unlike chemically vaccines. Hence, novelty of our candidate (OZG-38.61.3) is that it non-adjuvant added, gamma-irradiated, intradermally...
Hypoxia induces cell death through excessive production of reactive oxygen species (ROS) and calcium (Ca2+) influx in cells TRPM2 cation channel is activated by oxidative stress. Zinc (Zn), selenium (Se), glutathione (GSH) have antioxidant properties several hypoxia-induced activity, ROS may be inhibited the Zn, Se, GSH treatments. We investigated effects on lipid peroxidation (LPO), cytotoxicity inhibition activity transfected HEK293 exposed to hypoxia defined as deficiency.We induced four...
Introduction Rare and ultra-rare genetic conditions significantly contribute to infant morbidity mortality, often presenting with atypical features heterogeneity that complicate management. Rapid genome sequencing (RGS) offers a timely cost-effective approach diagnosis, aiding in early clinical management reducing unnecessary interventions. This pilot study represents the inaugural use of next-generation (NGS) as diagnostic instrument for critically ill neonatal pediatric ICU patients...
Abstract The clinical interpretation of missense variants is critically important in diagnostics due to their potential cause mild-to-severe effects on phenotype by altering protein structure. Evaluating these essential because they can significantly impact disease outcomes and patient management. Many computational predictors, known as silico pathogenicity predictors (ISPPs), have been developed support the assessment variant pathogenicity. Despite abundance ISPPs, predictions often lack...
Abstract Infants with congenital heart disease (CHD) often undergo thymectomy during corrective cardiac surgery (CCS). The long‐term immunological effects remain controversial, concerns regarding increased susceptibility to infections, allergies, autoimmunity due compromised immune tolerance mechanisms. This study aims elucidate the of early thymectomy. We enrolled 22 patients who underwent in infancy and were followed up Pediatric Allergy Immunology Clinic at Marmara University. performed...
Abstract Drugs and carcinogens are substrates of a group metabolic enzymes including cytochrome p450 gluthatione S‐transferases. Many the genes encoding these exhibit functional polymorphisms that contribute individual cancer susceptibility drug response. Molecular studies based on polymorphic also explain aetiology therapeutic management in clinics. We analysed p4501A1 ( CYP1A1 ) 2D6 CYP2D6 variant genotype allele frequencies by PCR‐RFLP Turkish individuals n = 140). The frequency CYP1A1*2A...
There is preliminary evidence that the local renin-angiotensin system (RAS) could affect neoplastic hematopoiesis. The aim of this study to search messenger RNA (mRNA) expressions essential RAS elements in myeloid and lymphoid hematological disorders. Forty-six patients with newly diagnosed (AML, biphenotypic leukemia, CML) or (CLL, NHL, B-ALL, T-ALL) disorders were included study. In group, median expression values RENIN, ACE1, ACE2 ANGIOTENSINOGEN (ANGTS) mRNAs 1.96%, 0.42%, 0.00% 0.00%,...
Tyrosine kinase inhibitor (TKI) therapy is the current treatment of choice for patients with chronic phase myeloid leukemia (CML) leading to rapid and durable hematological as well molecular responses. However, emergence resistance TKIs has been major obstacle success on long term. In this regard domain mutations are most common mechanism failure. study, we analyzed peripheral blood samples from 17 CML who had developed various by using next-generation sequencing parallel Sanger sequencing....
Objective: WNT5A is one of the most studied noncanonical WnT ligands and shown to be deregulated in different tumor types.Our aim was clarify whether hypermethylation might cause low mRnA levels we could restore this downregulation by reversing event.Materials Methods: The expression a large acute lymphoblastic leukemia (ALL) patient group (n=86) quantitative real-time PCR.The methylation status detected methylation-specific PCR (MSPCR) bisulphate sequencing.In order determine has direct...