A5084962593- Acute Lymphoblastic Leukemia research
- Chronic Myeloid Leukemia Treatments
- Cancer-related gene regulation
- Chronic Lymphocytic Leukemia Research
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Lymphoma Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
- Eosinophilic Disorders and Syndromes
- Genetics and Neurodevelopmental Disorders
- Bioinformatics and Genomic Networks
- Renal and related cancers
- Quinazolinone synthesis and applications
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- DNA Repair Mechanisms
- Childhood Cancer Survivors' Quality of Life
- Immunodeficiency and Autoimmune Disorders
- Genomics and Rare Diseases
- Cardiovascular Function and Risk Factors
- Wnt/β-catenin signaling in development and cancer
- Chemokine receptors and signaling
- Genomic variations and chromosomal abnormalities
- Cell death mechanisms and regulation
Istanbul University
2014-2025
Inserm
2022-2023
Université Paris-Saclay
2023
HUN-REN Institute of Experimental Medicine
2022
Queen's University Belfast
2012
WNT signaling has been implicated in the regulation of hematopoietic stem cells and plays an important role during T-cell development thymus. Here we investigated pathway activation childhood acute lymphoblastic leukemia (T-ALL) patients. To evaluate potential leukomogenesis, performed expression analysis key components pathway. More than 85% T-ALL patients showed upregulated β-catenin at protein level compared with normal human thymocytes. The impact this upregulation was reflected high...
Cellular functions are regulated and executed by complex protein interaction networks. Accordingly, it is essential to understand the interplay between proteins in determining activity status of signaling cascades. New methods therefore required provide information on different events at single cell level heterogeneous populations such as tissue sections. Here, we describe a multiplex proximity ligation assay for simultaneous visualization multiple complexes situ. The an enhancement original...
Summary TRIB 2 is a potent oncogene, elevated in subset of human acute myeloid leukaemias ( AML ) with mixed myeloid/lymphoid phenotype and NOTCH 1 mutations. Although rare , activating mutations occur 50% all T cell lymphoblastic ‐ ALL ). target gene that functions the degradation key proteins modulation MAPK signalling pathways, implicated haematopoietic survival proliferation. This study showed expression level highest lymphoid compartment normal cells, specifically cells. Analysis across...
The NOTCH signaling pathway plays important role in the development of multicellular organisms, as it regulates cell proliferation, survival, and differentiation. In adults, is essential for T- or B-lymphocyte lineage commitment. NOTCH1 FBXW7 mutations both lead activation are found majority T-ALL patients. this study, mutation analysis genes was performed 87 pediatric T-ALLs who were treated on ALL-BFM protocols. 19 patients (22%), activating observed either heterodimerization domain PEST 7...
Rare disease patients constitute a significant part of the healthcare system all countries. However, information on experiences during processes and daily life rare is still limited. So far, there small number studies conducted in Türkiye, they mainly cover specific issues like education or anxiety. Here we present comprehensive survey analysis among their families within scope Istanbul Solution Platform for Undiagnosed Diseases-ISTisNA project. A total 498 individuals responded to survey,...
Purpose: To model CML progression in vitro and generate a blast crisis (BC-CML) order to identify new targets. Methods: Three different CML-derived iPSC lines were mutagenized with the alkylating agent ENU on daily basis for 60 days. Cells analyzed at D12 of hematopoietic differentiation their phenotype, clonogenicity, transcriptomic profile. Single-cell RNA-Seq analysis has been performed three time points during ENU-treated untreated cells. Results: One CML-iPSCs, compared its...
The NOTCH signaling pathway plays important role in the development of multicellular organisms, as it regulates cell proliferation, survival, and differentiation. In adults, is essential for T- or B-lymphocyte lineage commitment. NOTCH1 FBXW7 mutations both lead activation are found majority T-ALL patients. this study, mutation analysis genes was performed 87 pediatric T-ALLs who were treated on ALL-BFM protocols. 19 patients (22%), activating observed either heterodimerization domain PEST 7...
The renin–angiotensin system (RAS) is involved in cell growth, proliferation and differentiation bone marrow an autocrine–paracrine manner, it modulates normal neoplastic haematopoietic proliferation. This study aimed to assess expressions of the RAS components, renin, angiotensinogen angiotensin-converting enzyme (ACE), during imatinib mesylate treatment patients with chronic myeloid leukaemia (CML). Expressions components were studied CML at time diagnosis ( n = 83) 3, 6 12 months after...
There is preliminary evidence that the local renin-angiotensin system (RAS) could affect neoplastic hematopoiesis. The aim of this study to search messenger RNA (mRNA) expressions essential RAS elements in myeloid and lymphoid hematological disorders. Forty-six patients with newly diagnosed (AML, biphenotypic leukemia, CML) or (CLL, NHL, B-ALL, T-ALL) disorders were included study. In group, median expression values RENIN, ACE1, ACE2 ANGIOTENSINOGEN (ANGTS) mRNAs 1.96%, 0.42%, 0.00% 0.00%,...
Tyrosine kinase inhibitor (TKI) therapy is the current treatment of choice for patients with chronic phase myeloid leukemia (CML) leading to rapid and durable hematological as well molecular responses. However, emergence resistance TKIs has been major obstacle success on long term. In this regard domain mutations are most common mechanism failure. study, we analyzed peripheral blood samples from 17 CML who had developed various by using next-generation sequencing parallel Sanger sequencing....
The tumor suppressor protein 53 (TP53) gene is one of the most studied genes in cancer. Although TP53 variants are rare events acute leukemia, recent observations showed that relapse samples might harbor variants. Here, we aimed to determine (hotspot region, exon 4-11) childhood lymphoblastic leukemia (B and T-ALL) patients (n = 94) including diagnostic-relapse pairs 15) by amplicon sequencing evaluate clinical impact these In total, nine different (E298*, R283C, R273H, L252F, C229F, I195T,...
Objective: The prominent functions of the local renin-angiotensin system (RAS) in primitive hematopoiesis further support hypothesis that autocrine bone marrow RAS could also be active neoplastic hematopoiesis.The aim this study is to examine critical elements normal CD34+ hematopoietic stem cells and multiple myeloma (MM)-related progenitor cells. Materials Methods:The group comprised total (CBM) 10 hematologically people, cell samples (CD34+CBM) 9 healthy donors for allogeneic peripheral...
B-cell precursor acute lymphoblastic leukemia (BCP-ALL) is a heterogeneous malignancy and consists of several genetic abnormalities. Some these abnormalities are used in clinics for risk calculation treatment decisions. Patients with ZNF384 rearrangements had distinct expression profile regardless their diagnosis, BCP-ALL or mixed phenotype (MPAL) defined as new subtype ALL. In this study, we screened 42 MPAL 91 patients the most common fusions; ZNF384::TCF3, ZNF384::EP300 ZNF384::TAF15 by...
T-cell acute lymphoblastic leukemia (T-ALL) is associated with recurrent chromosomal aberrations andabnormal ectopic gene expression during development. In order to gain insight into the pathogenesis of T-ALLthis study aimed measure level 7 oncogenes (LMO2, LYL1, TAL1, TLX1, TLX3, BMI1, andCALM-AF10) in pediatric T-ALL patients Material and Methods: LMO2, CALM-AF10 was measured usingquantitative real-time PCR 43 patients.A high BMI1 genes observed a large group T-ALL.Several signatures...
Background/aim: The canonical Wingless-type (WNT) pathway is involved in normal hematopoietic cell development and deregulated WNT signaling implicated the of hematological malignancies. Dickkopf 1 (DKK1) acts as a modulator β-catenin regulated pathway. Activation DKK1 leads to apoptosis growth suppression, whereas silencing by promoter hypermethylation results abnormal activation. secreted inhibitor can antagonize competitively binding low density lipoprotein receptors (LRPs) 5 6. Materials...
Objective: Despite the efficacy of BCR-ABL tyrosine kinase inhibitor imatinib, development resistance against imatinib has been observed.The most important mechanisms known to cause are point mutations in ABL and ATP domain.This study describes use denaturing high performance liquid chromatography (dHPLC) as a method screen for gene.Material Methods: We used dHPLC based assay screening mutations.Forty chronic myeloid leukemia (CML) patients who showed were screened parallel by direct...
Abstract Introduction The lymphoid enhancer factor 1 (LEF1) is a DNA‐binding transcription that functions in the Wnt signaling pathway. Increased LEF1 activity associated with progression of several types cancer including leukemia. Here, we investigated isoform expression and genomic variations acute lymphoblastic leukemia (ALL). Methods was evaluated by quantitative real‐time PCR 87 newly diagnosed childhood ALL patients controls. Moreover, Western blot analysis performed for detection...
The current treatment of chronic phase myeloid leukemia (CML) consists oral tyrosine kinase inhibitors (TKIs). However, high-risk CML may present with an aggressive course which result in blastic crisis or a "difficult-to-manage" state available treatments. aim this paper is to report patient complicated resistant and progressed despite the administration bosutinib, imatinib mesylate, nilotinib, dasatinib, interferon alpha 2a, cytotoxic chemotherapy, allogeneic hematopoietic stem cell...