Background/Aim: Prostate cancer is the second leading cause of cancer-related deaths in men. Periodontitis considered a high-risk factor for prostate cancer, but genetic mechanism unclear. This study aims to identify dysregulated miRNAs, their associated genes, signaling pathways, and compounds linking periodontitis cancer. Materials Methods: The miRNA expression datasets were obtained from GEO database. Differentially expressed miRNAs (DEmiRNAs) identified, common DEmiRNAs (Co-DEmiRNAs)...

The use of Lucilia sericata larvae on the healing wounds in diabetics has been reported. However, role excretion/secretion (ES) products treatment diabetic remains unknown. This study investigated whether application ES L. wound surface could improve impaired streptozotocin-induced rats. Additional analysis was performed to understand proteome content secretome contribution at molecular level. For this purpose, full-thickness skin were created backs and control A conducted assess levels...

Small cell lung carcinoma (SCLC) is a highly aggressive cancer with low survival rate. Although initial response to chemotherapy in SCLC patients well-rated, the treatments applied after disease relapses are not successful. Drug resistance accepted be one of main reasons for this failure. Therefore, there an urgent need new treatment strategies SCLC. Meclofenamic acid, nonsteroidal anti-inflammatory drug, has been shown have anticancer effects on various types cancers via different...

<i>Objective:</i> Cisplatin (DDP, <i>cis</i>-diamminedichloroplatinium II) is one of the most potent chemotherapeutic antitumor drugs, but able to generate reactive oxygen species (ROS) and it also inhibits activity antioxidant enzymes in renal tissue. In present study, we investigated preventive effect 100, 200 400 mg/kg b.w. doses vitamin E (VE), 25, 50, 100 A (VA) combination on malondialdehyde (MDA), nitric oxide (NO), glutathione (GSH) levels superoxide dismutase...

The present study was carried out to evaluate the effects of 1alpha,25-dihydroxyvitamin D3 (VD) on chromosomal aberrations induced by doxorubicin (DXR). Wistar rats were divided into eight experimental groups five animals each. Control group treated with i.p. distilled water. in three VD given only for 4, 6 or 8 weeks. In DXR DXR. combination doses weeks each and injected 24 h before sacrificing rats. (50 mg/100 g b.w.) intraperitoneally gavage 3 microg/kg/day twice weekly. Animals both...

This prospective randomized study investigated the effects of two different cardioplegia techniques on myocardial heat shock protein 70 (HSP70) mRNA levels. Patients undergoing elective coronary artery bypass grafting with cardiopulmonary (CPB) were divided into equal groups. All patients received same anaesthesia. Myocardial preservation was achieved by delivering intermittent antegrade isothermic blood in one group and plus continuous retrograde other. Biopsies for measurement HSP70 levels...

Fat mass and obesity-associated protein is an enzyme that oxidatively demethylates DNA. Although there are numerous studies regarding the catalytic function of FTO, overall existence or absence FTO on cellular proteome has not been investigated. This study investigated changes in soluble 3T3-L1 cells upon expression WT mutant (R316Q) proteins. Protein extracts prepared from expressing either proteins were used DIGE experiments. Analysis data revealed number spots matched to every member 350...

Abstract Tetracycline regulated protein expression in mammalian cells is a powerful tool to predict the physiological function, cellular localization, and stability of protein. In addition, metabolic networks affected by wild‐type or mutant forms proteins, researchers generally produce single cell clone that can express interest under tetracycline control study changes occurring overall proteome before after interest. One limitation clonal creation, however, it sometimes creates clones with...

Ureteropelvic junction (UPJ) obstruction is a common problem in children, but its etiology remains unclear. In this study, the proteome profiles of obstructed segment and surrounding distal proximal parts were comparatively evaluated. Twelve children younger than 2 years age with unilateral intrinsic UPJ included. The excised operational tissue was divided into three immediately after resection: part (Obst), normal ureteral (Dist), (Prox). Proteins extracted from samples subjected to...

Objective: Fat mass and obesity associated (FTO) protein is an RNA-demethylase which employed in various metabolic functions such as post-transcriptional modifications, DNA repair fatty acid utilization. was initially found to be closely with increased body-mass-index later studies have established association of FTO neurological diseases cancer. The aim this study investigate the effect R316Q mutation on soluble proteome SH-SY5Y cells.Methods: cells stably expressing wild-type (WT) mutant...

ÖZ GİRİŞ ve AMAÇ: Akut myeloid löseminin (AML) 1alpha,25dihydroxyvitamin D (3) (1,25(OH)2D3)'ün farklılaştırma etkisiyle tedavisi ön plana çıkan araştırma konularındandır.Bu çalışmanın amacı HL-60 hücrelerinde lösemi hücre döngüsü regülasyonu apopitozunda 1,25(OH)2D3'ün rolünü araştırmaktır. YÖNTEM GEREÇLER:Vitamin ile indüklenmiş ücre apoptoz ilişkili 13 genin (TNFR1, Bax, Bak, AIF, Survivin, Cdk1 (Cdc2), Cdk2, Cdk4, Siklin D1 E) dört farklı zamanda (18, 36, 48, 72.saatler) gen...

Objective: Proteomic approaches, have been used in monitoring the disease processes and identification of biomarkers. Biological samples field proteomics include biological fluids solid samples. In this context, pre-analytical e.g., tissue sample quality, sampling site accuracy transfer time to proteomic laboratory are very important. study, proteome profiles taken from two different regions within a tumor were compared show heterogeneity.
 Material Methods: Proteins obtained...

Objectives: Despite various studies to improve the early detection and development of novel treatment agents, no significant progress has been made in prostate cancer. Moreover, metastasis carcinoma is a major challenge modalities. Therefore, present study, it was aimed investigate effect meclofenamic acid (MA), nonsteroidal anti-inflammatory drug, on migration invasion LNCaP cells.
 Methods: Firstly, non-toxic concentrations MA cells were determined by trypan blue exclusion assay....