A5000403042- Diabetes and associated disorders
- Pancreatic function and diabetes
- Diabetes Management and Research
- Pluripotent Stem Cells Research
- Pancreatic and Hepatic Oncology Research
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- CRISPR and Genetic Engineering
- Carbohydrate Chemistry and Synthesis
- Immune cells in cancer
- Glycosylation and Glycoproteins Research
- Pancreatitis Pathology and Treatment
- Cancer Immunotherapy and Biomarkers
- Connective tissue disorders research
- Acute Myeloid Leukemia Research
- Galectins and Cancer Biology
University of Washington
2019-2024
University of California, San Diego
2022-2024
Salk Institute for Biological Studies
2022-2024
City of Hope
2014-2018
Beckman Research Institute
2014-2018
Diabetes Australia
2013
The study of hematopoietic colony-forming units using semisolid culture media has greatly advanced the knowledge hematopoiesis. Here we report that similar methods can be used to pancreatic units. We have developed two colony assays enable quantitative and functional analyses progenitor-like cells isolated from dissociated adult (2-4 mo old) murine pancreas. find a methylcellulose-based medium containing Matrigel allows growth duct-like "Ring/Dense" colonies rare (∼1%) population total...
Progenitor cells in the adult pancreas are potential sources of endocrine beta for treating type 1 diabetes. Previously, we identified tri-potent progenitor (2-4month-old) murine that were capable self-renewal and differentiation into duct, acinar, vitro. These named pancreatic colony-forming units (PCFUs). However, because PCFUs a minor population (~1%) they difficult to study. To enrich PCFUs, strategies using cell-surface marker analyses fluorescence-activated cell sorting developed. We...
Postnatal pancreas is a potential source for progenitor cells to generate endocrine β-cells treating type 1 diabetes. However, it remains unclear whether young (1-week-old) harbors multipotent progenitors capable of differentiating into duct, acinar, and cells. Laminin an extracellular matrix (ECM) protein important β-cells' survival function. We established artificial (aECM) that contains the functional IKVAV (Ile-Lys-Val-Ala-Val) sequence derived from laminin (designated aECM-lam). Whether...
The Review of Diabetic Studies,2014,11,1,35-50.DOI:10.1900/RDS.2014.11.35Published:May 2014Type:Original ArticleAuthors:Liang Jin, Tao Feng, Jing Chai, Nadiah Ghazalli, Dan Gao, Ricardo Zerda, Zhuo Li, Jasper Hsu, Alborz Mahdavi, David A. Tirrell, Arthur D. Riggs, and Hsun Teresa Ku Author(s) affiliations:Liang Jin1,*, Feng1,*, Chai1,*, Ghazalli1,2, Gao1, Zerda3, Li3, Hsu1, Mahdavi4, Tirrell5, Riggs1, Ku1,2 1Department Diabetes Metabolic Diseases Research, Beckman Research Institute, City...
Pluripotent stem cells may serve as an alternative source of beta-like for replacement therapy type 1 diabetes; however, the generated in many differentiation protocols are immature. The maturation endogenous beta involves increase insulin expression starting late gestation and a gradual acquisition abilities to sense glucose secrete by week 2 after birth mice; what molecules regulate these processes incompletely known. In this study, we aim identify small that affect immature cells. A...
Abstract Pancreatic ductal adenocarcinoma (PDA) is an intractable common malignancy with a pro-tumorigenic and immunosuppressive microenvironment (ME) that often limits treatment efficacy. Only 10-15% of patients are eligible for surgical resection, which the only cure PDA. The glycan CA19-9 used to follow response in PDA patients, but its functional role remained unknown until recently because rodents lack ability produce this carbohydrate. elevation KRAS-mutant background results increased...
Abstract Pancreatic ductal adenocarcinoma (PDA) is a highly lethal malignancy with five-year survival rate of less than 11%, making it one the deadliest cancers. Notably, 95% PDA cases exhibit KRAS mutations, contributing to dysregulated cell signaling networks. Despite extensive research into interplay various oncogenic pathways in pancreatic tumorigenesis, targeted therapies against these networks have displayed modest efficacy, most patients rapidly developing therapeutic resistance....
Abstract Pancreatic cancer is a deadly malignancy. We have setup pipeline to interrogate cell intrinsic and extrinsic mechanisms contributing treatment response. Using combination of mouse human organoids models together with in vivo investigation, we systematically dissected key signaling hubs driving pro-tumorigenic features as well remodeling the tumor microenvironment (TME). found that glycan epitope, CA19-9, drives pancreatic inflammation, cancer, metastasis. CA19-9 part through...
Abstract Background and Aims Chronic pancreatitis (CP) affects more than 200,000 Americans 1 million individuals world wide, but treatment generally focuses on supportive care like pain management. 28-80% of CP cases are idiopathic 10-15% hereditary with mutations in PRSS1, SPINK1, CFTR, other genes. Developing human models to understand the drivers disease targeting key players this will provide novel therapeutic approaches for these patients who have limited options. Methods Utilizing...
Abstract Pancreatic ductal adenocarcinoma (PDA) is an intractable common malignancy with a pro-tumorigenic and immunosuppressive microenvironment (ME) that often limits treatment efficacy. The glycan CA19-9 used to follow response in PDA patients, but its functional role remained unknown until recently because rodents lack this carbohydrate. elevation KRAS-mutant background promotes rapid progression invasive carcinoma induces ME remodeling mice. tumor includes cancer associated fibroblasts...