A5030913747- Pancreatic function and diabetes
- Diabetes Management and Research
- Diabetes and associated disorders
- Pancreatic and Hepatic Oncology Research
- Extracellular vesicles in disease
- Science Education and Pedagogy
- RNA modifications and cancer
- Galectins and Cancer Biology
- Phagocytosis and Immune Regulation
- Genetics, Bioinformatics, and Biomedical Research
- Animal Genetics and Reproduction
- MicroRNA in disease regulation
- Cancer Mechanisms and Therapy
- Pluripotent Stem Cells Research
- Glioma Diagnosis and Treatment
- Carbohydrate Chemistry and Synthesis
- Angiogenesis and VEGF in Cancer
- Glycosylation and Glycoproteins Research
City of Hope
2015-2024
Beckman Research Institute
2015-2024
Salk Institute for Biological Studies
2022
Point Loma Nazarene University
2012-2021
Progenitor cells in the adult pancreas are potential sources of endocrine beta for treating type 1 diabetes. Previously, we identified tri-potent progenitor (2-4month-old) murine that were capable self-renewal and differentiation into duct, acinar, vitro. These named pancreatic colony-forming units (PCFUs). However, because PCFUs a minor population (~1%) they difficult to study. To enrich PCFUs, strategies using cell-surface marker analyses fluorescence-activated cell sorting developed. We...
Progenitor cells capable of self-renewal and differentiation in the adult human pancreas are an under-explored resource for regenerative medicine. Using micro-manipulation three-dimensional colony assays we identify within exocrine that resemble progenitor cells. Exocrine tissues were dissociated into single plated a assay containing methylcellulose 5% Matrigel. A subpopulation ductal formed colonies differentiated ductal, acinar, endocrine lineage cells, expanded up to 300-fold with ROCK...
Adult pancreatic stem and progenitor cells may serve as an alternative source of insulin-secreting endocrine in cell replacement therapy for type 1 diabetes, but much remained unknown about these cells. We previously identified adult murine progenitor-like that displayed vitro self-renewal tri-lineage differentiation activities a three-dimensional colony/organoid assay containing 1% methylcellulose 5% Matrigel. However, the presence other undefined culture components, such serum conditioned...
Abstract Pancreatic ductal progenitor cells have been proposed to contribute adult tissue maintenance and regeneration after injury, but the identity of such remains elusive. Here, from mice, we identify a near homogenous population progenitor-like clusters, with an average 8 per cluster. They are rare subpopulation, about 0.1% total pancreatic cells, can be sorted using fluorescence-activated cell sorter CD133highCD71lowFSCmid-high phenotype. exhibit properties in self-renewal tri-lineage...
We describe a technique for removing and growing chick embryos in culture that utilizes relatively inexpensive materials requires little space. It can be readily performed class by university, high school, or junior students, teachers of any grade level should able to set it up their students. Students will directly observe the chick’s development from 3 days post-fertilization point at which would normally hatch. Observing embryonic first hand, including embryos’ natural movements, gives...
Stem and progenitor cells from the adult pancreas could be a potential source of therapeutic beta-like for treating patients with type 1 diabetes. However, it is still unknown whether stem exist in pancreas. Research strategies using cre-lox lineage-tracing mice have yielded results that either support or refute idea beta can generated ducts, presumed location where pancreatic progenitors may reside. These vivo methods, however, cannot answer questions self-renewal multi-lineage...
The existence and regenerative potential of resident stem progenitor cells in the adult pancreas are controversial topics. A question that has been only minimally addressed is capacity a cell to self-renew, key attribute defines cells. Previously, our laboratory identified putative from murine pancreas. Using an
Tumor angiogenesis is critical for the growth and progression of cancer. As such, angiostasis a treatment modality cancer with potential utility multiple types fewer side effects. However, clinical success angiostatic monotherapies has been moderate, at best, causing treatments to lose their early luster. Previous studies demonstrated compensatory mechanisms that drive tumor vascularization despite use monotherapies, as well combination therapies overcome these mechanisms. We screened...
Stem and progenitor cells from the adult pancreas could be a potential source of therapeutic beta-like for treating patients with type 1 diabetes. However, it is still unknown whether stem exist in pancreas. Research strategies using cre-lox lineage-tracing mice have yielded results that either support or refute idea beta can generated ducts, presumed location where pancreatic progenitors may reside. These vivo methods, however, cannot answer questions self-renewal multi-lineage...
Abstract Pancreatic ducts function to deliver digestive enzymes into the intestines. Upon injury, can become proliferative and contribute tissue regeneration; however, identity of ductal cells that these processes is unknown. We combined fluorescence-activated cell sorting, a methylcellulose-containing 3-dimensional culture, droplet RNA-sequencing, clonal lineage tracing tool identify isolate distinct subpopulation pancreatic exhibit progenitor properties. These are unique in they form...