A5101848562- Herpesvirus Infections and Treatments
- interferon and immune responses
- Cytomegalovirus and herpesvirus research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Plant Virus Research Studies
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- Animal Disease Management and Epidemiology
- Protein Hydrolysis and Bioactive Peptides
- RNA regulation and disease
- T-cell and Retrovirus Studies
- Microbial Community Ecology and Physiology
- Salmonella and Campylobacter epidemiology
- Probiotics and Fermented Foods
- Advanced biosensing and bioanalysis techniques
- GABA and Rice Research
- Toxin Mechanisms and Immunotoxins
- Antibiotic Resistance in Bacteria
- Helminth infection and control
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Metal Extraction and Bioleaching
Linyi University
2018-2024
Guangxi University
2015-2021
A total of 81 clinical cases suspected tumours were submitted to our laboratory from Yellow chicken farms in southern China during the years 2010 through 2017. The tumour-like tissue samples closely examined for common oncogenic avian viruses cell culture and further analysed using polymerase chain reaction (PCR). During 2010-2012, Marek's disease virus (MDV) mono-infection was found be dominant cause tumour incidences (52.4%, 11/21) followed by co-infection MDV+ALVs (19.1%, 4/21). Starting...
One avian leukosis virus subgroup J (ALV-J) strain was isolated from 67 commercial live poultry vaccines produced by various manufacturers during 2013–2016 in China. The complete genomes of the isolate were sequenced and it found that genes gag pol relatively conservative, while gp85 gene GX14YYA1 had highest similarities with a field GX14ZS14, which chickens farm once used same vaccine as one to be contaminated GX14YYA1. This is first report ALV-J contaminant Our finding demonstrates...
Abstract Avian leukosis virus subgroup J (ALV-J) has infected a variety of birds, causing major economic losses in China. Understanding the comprehensive criteria classification and nomenclature ALV-J would be useful for investigation viral evolution also prevention control this infection. An in-depth analysis genetic diversity was performed present study. Four hundred seventy-five sequences gp85 gene, including thirteen avian endogenous retrovirus designated ev/J 462 ALV-J, were used...
Marek’s disease (MD) is an infectious malignant T-cell lymphoma proliferative caused by virus (MDV). In recent years, the emergence of very virulent (vv) and/or plus (vv +) strains MDV in field has been suggested as one causes vaccination failure. The pathogenicity strain GX18NNM4, isolated from a clinical outbreak broiler breeder flock that was vaccinated with CVI988/Rispens, investigated. vaccination-challenge test, GX18NNM4 able to break through protections provided vaccines CVI988 and...
Marek's disease (MD) continues to threaten the sustainability of world poultry industry. In this study, sequences meq gene 220 MDV strains isolated during years 1964–2020 were analysed, including 50 from our group plus 170 isolates GenBank. Analyses, using phylogenetic trees, amino acid (aa)-mutation screening, evolutionary studies and transmission dynamics all performed. All divided into two clusters (Clusters 1 2), Cluster includes mild strains, vaccine foreign virulent while 2 was...
During the process of transmission and spread avian leukosis virus subgroup J (ALV-J) in chickens worldwide, viral genome is constantly changing. A comprehensive systematic study evolutionary ALV-J China needed. In this study, we amplified full-length cDNA sequences 16 isolates Yellow-chicken origin analyzed compared these with another 69 strains isolated during years 1988–2018. These were then sorted into 2 clusters: cluster I included that mainly originated from layers White-feather...
Abstract The coinfection of ALVs (ALV-J plus ALV-A or/and ALV-B) has played an important role in the incidence tumors recently found China local breeds yellow chickens. study aims to obtain a better knowledge function and relevance ALV clinical disease avian leukosis, as well its unique effect on pathogenicity Three-yellow One-day-old chicks (one day old) were infected with ALV-A, ALV-B, ALV-J mono-infections, + J, ALV-B B J coinfections, via intraperitoneal injection, then grown isolators...
The facultative anaerobic Gram-positive bacterium
The geographical spread and inter-host transmission of the subgroup J avian leukosis virus (ALV-J) may be most important issues for epidemiology. An integrated analysis, including phylogenetic trees, homology modeling, evolutionary dynamics, selection analysis viral transmission, based on gp85 gene sequences 665 worldwide ALV-J isolates during 1988-2020, was performed. A new Clade 3 has been emerging evolved from dominating 1.3 Chinese Yellow-chicken, loss a α-helix or β-sheet protein...
One natural recombinant avian leukosis virus (ALV) strain GX14DJ3–18 was isolated from a native gamecock by DF-1 cell culture and identified with Polymerase Chain Reaction (PCR), immunofluorescence assay the viral genome's nucleotide sequencing. This revealed as novel sequence similarities of 95.4% Long Terminal Repeated (LTR), 95.8% 5′, UTR, 97.9% gag, 92.9% 3′untranslated regions (UTR) in ALV-J. Also we found 99.3% pol 99.0% gp37 ALV-E, 89.9% gp85 ALV-A. The simulated congenital infection...
Both reticuloendotheliosis and Marek's disease are neoplastic diseases of chickens caused by virus (REV) (MDV), respectively. The infection REV or MDV may lead to clinical tumors also result in immunosuppression easily allow secondary other pathogens. Here, we investigated a breeder flock three-yellow southern China that had been vaccinated with CVI988/Rispens at hatching experienced depression, weakness, reduction weight gain, an increased death rate after 120 d age. morbidity mortality...
Haemonchus contortus is an important parasite that causes disease seriously endangers ruminant animals cattle, sheep, goat, and camel. Here, we compared the proeomics analysis of three adult isolates from mouflons ( Ovis ammon ). A total 1,299 worm proteins were identified, 461 quantified, which 82 (108), 83 (97), 97 (86) significantly upregulated (downregulated) differentially expressed (DEPs) detected among pairwise comparisons (1-vs.-3, 2-vs.-3, 2-vs.-1). Liquid chromatography–tandem mass...
Subgroups A, B, E and J are the major subgroups of avian leukosis virus (ALV) infecting chickens. ALV infection has become endemic in China a significant negative effect on poultry industry. Consequently, there is an urgent need for specific, sensitive rapid method diagnosis eradication ALV. Therefore, we developed simple real-time loop-mediated isothermal amplification (LAMP) reaction timely detection common subgroups, whereby can be obtained 35 min under conditions at 63 °C, ability to...
One avian leukosis virus of subgroup J (ALV-J) strain GX14YYA1 was isolated from a commercial bivalent Newcastle disease (ND)–infectious bronchitis (IB) vaccine in our previous study. To evaluate the pathogenicity ALV-J-contaminated on chickens, day-old Three-Yellow chicks group I were vaccinated with ND-IB live by intranasal and eye drop at 1-day-old for primary vaccination 7-day-old secondary vaccination. Groups II III kept as normal noncontaminated blank control groups, respectively. The...
The reticuloendotheliosis virus (REV) and the Marek's disease (MDV) cause (RE) (MD) in poultry, respectively. According to epidemiological results obtained our laboratory from 2010 2017, positive rates of REV MDV co-infection remained at low levels. In present study, during period October 2018 July 2020, 4 clinical cases with high morbidity (5%-20%) mortality (2%-10%), caused by vv+ MDV-like strains, were diagnosed analyzed histopathological observation, cell cultures detection ELISA IFA,...
This study aims to investigate the probiotic properties of various isolated strains Lactiplantibacillus plantarum. Specifically, focus is on examining expression glutamic acid decarboxylase (GAD) gene and its role in production gamma-aminobutyric (GABA), a promising postbiotic metabolite. The investigation includes comprehensive analyses morphology, genetics, resilience against bile, NaCl, simulated pancreatin juice (SPJ), carbohydrate fermentation patterns, antibacterial activity,...
Multidrug-resistant (MDR) Acinetobacter baumannii (A. baumannii) is currently recognized not only as a significant nosocomial pathogen but also an emerging bacterial infection in food-producing animals, posing critical threat to global health. However, this hindrance detailed bioinformatic studies of MDR A. chicken origin due the lack its complete genome sequence. Here, we report whole-genome sequencing analysis Y03 isolated from chickens. The consists 1 circular chromosome and 4 plasmids,...