A5100701724- Retinal Development and Disorders
- Renin-Angiotensin System Studies
- Retinal Diseases and Treatments
- Gut microbiota and health
- Receptor Mechanisms and Signaling
- interferon and immune responses
- Virus-based gene therapy research
- Retinal and Optic Conditions
- Ocular Diseases and Behçet’s Syndrome
- Herpesvirus Infections and Treatments
- Systemic Lupus Erythematosus Research
- Adenosine and Purinergic Signaling
- Nitric Oxide and Endothelin Effects
- Hormonal Regulation and Hypertension
- Neuroinflammation and Neurodegeneration Mechanisms
- Nutrition, Genetics, and Disease
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Diet and metabolism studies
- Protein Hydrolysis and Bioactive Peptides
- Chronic Kidney Disease and Diabetes
- CRISPR and Genetic Engineering
- Oral microbiology and periodontitis research
- Proteins in Food Systems
- Advanced Fluorescence Microscopy Techniques
- Transgenic Plants and Applications
Guizhou University
2024
Tianjin Medical University
2022-2024
Southern Medical University
2024
Nanfang Hospital
2024
Air Force Medical University
2019-2024
Xijing Hospital
2019-2024
Hunan Normal University
2024
University of Florida
2014-2023
Institute of Endocrinology
2022
Guangxi University
2020-2022
Vectors derived from adeno-associated viruses (AAVs) have become important gene delivery tools for the treatment of many inherited ocular diseases in well-characterized animal models. Previous studies determined that viral capsid plays an essential role cellular tropism and efficiency transgene expression. Recently, it was shown phosphorylation surface-exposed tyrosine residues AAV2 targets particles ubiquitination proteasome- mediated degradation, mutations these lead to highly efficient...
Accumulating evidence indicates a key role of inflammation in hypertension and cardiovascular disorders. However, the inflammatory processes neurogenic remains to be determined. Thus, our objective present study was test hypothesis that activation microglial cells generation proinflammatory cytokines paraventricular nucleus (PVN) contribute hypertension. Intracerebroventricular infusion minocycline, an anti-inflammatory antibiotic, caused significant attenuation mean arterial pressure,...
Intermittent fasting (IF) protects against the development of metabolic diseases and cancer, but whether it can prevent diabetic microvascular complications is not known. In
Vectors based on adeno-associated virus serotype 2 (AAV2) have been used extensively in many gene-delivery applications, including several successful clinical trials for one type of Leber congenital amaurosis the retina. Many studies focused improving AAV2 transduction efficiency and cellular specificity by genetically engineering its capsid. We previously shown that vectors-containing single-point mutations capsid surface tyrosines serotypes AAV2, AAV8, AAV9 displayed significantly...
Gut microbiota** play important roles in the health and disease status of both humans animals. Little is known about whether heat stress changes composition gut microbiota chicken. The aim this study was to investigate effects on caecal microbiota, including growth performance as well HSP70 cortisol levels. Sixty 14-day-old female broilers were equally divided into 2 treatment groups with different housing temperatures for 28 D: a control group (C) at 24 26°C (HS) 34 38°C. contents broiler...
Despite evidence that hyperactivity of the vasodeleterious axis (ACE/angiotensin II (Ang II)/AT1 receptor) renin-angiotensin system (RAS) is associated with pathogenesis diabetic retinopathy (DR) use inhibitors this has met limited success in control pathophysiology. We investigated hypothesis enhancing local activity recently established protective RAS, ACE2/Ang-(1-7), using adeno-associated virus (AAV)-mediated gene delivery ACE2 or Ang-(1-7) would confer protection against...
Emerging evidences indicate that diminished activity of the vasoprotective axis renin-angiotensin system, constituting angiotensin-converting enzyme 2 (ACE2) and its enzymatic product, angiotensin-(1-7) [Ang-(1-7)] contribute to pathogenesis pulmonary hypertension (PH). However, long-term repetitive delivery ACE2 or Ang-(1-7) would require enhanced protein stability ease administration improve patient compliance. Chloroplast expression therapeutic proteins enables their bioencapsulation...
The angiotensin converting enzyme 2 (ACE2) catalyzes the degradation of Angiotensin II (Ang II) to generate Angiotensin-(1-7), which reduces inflammation and oxidative stress stimulated by Ang II. ACE2 has been shown be protective in cardiovascular metabolic diseases including diabetes its complications. However, challenge for clinical application is large-scale production high-quality with sufficient target tissue bioavailability. We developed an expression delivery system based on use...
Gene therapy represents an attractive approach for the treatment of eye diseases such as glaucoma. Ocular administration viral vectors produces localized retinal gene expression with reduced risks side effects reported systemic vectors. Recombinant adeno-associated (AAV) have proven effective in producing long-term expression, due to stable integration DNA into genome and lack host immune response virus. Recently developed AAV constructs using chicken β-actin (CBA) promoter drive highly...
Dysfunction of endothelial nitric oxide synthase (eNOS) has been implicated in the pathogenesis diabetic vascular complications. This study was undertaken to determine role eNOS development retinopathy (DR), by investigating functional consequences its deficiency state.Diabetes induced eNOS-knockout (eNOS(-/-)) and C57B/6 mice streptozotocin (STZ) injection. Retinal vasculature evaluated albumin extravasation, quantitatively measure permeability, trypsin-digested retinal preparations,...
Rationale: Despite overwhelming evidence of the importance brain renin–angiotensin system (RAS), very existence intrinsic RAS remains controversial. Objective: To investigate hypothesis that (pro)renin receptor (PRR) is physiologically important in regulation and cardiovascular functions. Methods Results: PRR broadly distributed within neurons cardiovascular-relevant regions. The physiological functions were studied supraoptic nucleus (SON) because this region showed greater levels mRNA...
Uveitis is a common cause of vision loss. The renin angiotensin system (RAS), which plays vital role in cardiovascular system, potent mediator inflammation and has been implicated the pathogenesis uveitis. A newly identified axis RAS, ACE2/Ang-(1-7)/Mas, emerged as novel target because it counteracts deleterious effect II. purpose this study was to investigate endogenous ACE2 activation preventing endotoxin-induced uveitis (EIU) mice.ACE2 activator diminazene aceturate (DIZE) administered...
Hyperactivity of the renin-angiotensin system (RAS) resulting in elevated Angiotensin II (Ang II) contributes to all stages inflammatory responses including ocular inflammation. The discovery angiotensin-converting enzyme 2 (ACE2) has established a protective axis RAS involving ACE2/Ang-(1–7)/Mas that counteracts proinflammatory and hypertrophic effects deleterious ACE/AngII/AT1R axis. Here we investigated hypothesis enhancing systemic local activity by oral delivery ACE2 Ang-(1–7)...
Delivering neurotherapeutics to target brain-associated diseases is a major challenge. Therefore, we investigated oral delivery of green fluorescence protein (GFP) or myelin basic (MBP) fused with the transmucosal carrier cholera toxin B subunit (CTB), expressed in chloroplasts (bioencapsulated within plant cells) brain and retinae triple transgenic Alzheimer's disease (3×TgAD) mice, across blood-brain barriers (BBB) blood-retinal (BRB). Human neuroblastoma cells internalized GFP when...
Therapeutic advances for pulmonary hypertension (PH) have been incremental because of the focus on vasculature in PH pathology. Here, we evaluate concept that is, rather, a systemic disorder involving interplay among multiorgan systems, including brain, gut, and lungs. Therefore, objective this study was to hypothesis is associated with dysfunctional brain-gut-lung axis global overexpression ACE2 (angiotensin-converting enzyme 2) rebalances protects against PH. knockin wild-type (WT;...
Inhibition of vascular endothelial growth factor (VEGF) for the management pathological ocular neovascularization associated with diseases such as neovascular age-related macular degeneration is a proven paradigm; however, monthly intravitreal injections are required optimal treatment. We have previously shown that novel, secreted anti-VEGF molecule sFLT01 delivered by injection an AAV2 vector (AAV2-sFLT01) gives persistent expression and efficacious in murine model retinal...
Endothelial progenitor cells (EPCs), critical for mediating vascular repair, are dysfunctional in a hyperglycemic and/or hypercholesterolemic environment. Their dysfunction contributes to the progression of diabetic macro- and microvascular complications. Activation "cholesterol-sensing" nuclear receptors, liver X receptors (LXRα/LXRβ), protects against atherosclerosis by transcriptional regulation genes important promoting cholesterol efflux inhibiting inflammation. We hypothesized that LXR...