A5050557647- Wound Healing and Treatments
- Mesenchymal stem cell research
- Laser Applications in Dentistry and Medicine
- Dermatologic Treatments and Research
- Electrospun Nanofibers in Biomedical Applications
- Retinal Imaging and Analysis
- Periodontal Regeneration and Treatments
- Intraocular Surgery and Lenses
- Ophthalmology and Visual Impairment Studies
- Angiogenesis and VEGF in Cancer
- Glaucoma and retinal disorders
- Body Contouring and Surgery
- Lymphatic System and Diseases
- Mast cells and histamine
- Melanoma and MAPK Pathways
- Optical Imaging and Spectroscopy Techniques
- Reconstructive Surgery and Microvascular Techniques
- Neural Networks and Applications
- Retinal and Optic Conditions
- Fibroblast Growth Factor Research
- Cytokine Signaling Pathways and Interactions
- Cancer Cells and Metastasis
- Radiopharmaceutical Chemistry and Applications
- Tissue Engineering and Regenerative Medicine
- Brain Tumor Detection and Classification
First Affiliated Hospital of Jinan University
2016-2025
Affiliated Hospital of North Sichuan Medical College
2020-2025
North Sichuan Medical University
2020-2025
National University of Defense Technology
2023-2025
China Medical University Hospital
2023
China Medical University
2023
Jinan University
2022
Institute of Economics
2022
Second Xiangya Hospital of Central South University
2021
Central South University
2021
Background Wound healing is a complex process that relies on growth factors and stimulation of angiogenesis. Tissue engineering materials composed adipose-derived stem cells (ADSCs) silk fibroin (SF)/chitosan (CS) may be able to solve this problem. The aim study was investigate the wound-healing potential ADSC-seeded SF/CS in streptozotocin-induced diabetic rats. Materials Methods Thirty-six male Sprague-Dawley rats were purchased randomly assigned into 3 groups: control group (no graft),...
Adipose-derived stem cells (ADSCs) have been documented as possible candidates for skin rejuvenation. However, the effects of ADSC-derived exosomes on photoaged remain to be fully elucidated. This study was aimed at determining antiaging skin. Human ADSCs were isolated from adipose tissue healthy women and cultured in vitro. Then, extracted ADSCs, purified by ultracentrifugation, verified examination cell morphology using transmission electron microscopy identification specific biomarkers....
Angiogenesis is crucial in diabetic wound healing and often impaired foot ulcers (DFUs). Human dermal microvascular endothelial cells (HDMECs) are vital components angiogenesis; however, their functional transcriptomic characteristics DFU patients not well understood. This study aimed to comprehensively analyse HDMECs from healthy controls find the potential regulator of angiogenesis DFUs.HDMECs were isolated skin specimens via magnetic-activated cell sorting. The proliferation, migration...
The present study was conducted to investigate the effects of helium-neon (He-Ne) laser irradiation on proliferation, migration, and differentiation cultured human epidermal stem cells (ESCs).A He-Ne with a wavelength 632.8 nm is known have photobiological effects, widely used for accelerating wound healing; however, cellular mechanisms involved not been completely understood.The ESCs were prepared from foreskin, irradiated by using at 2 J/cm(2). ESC examined XTT assay, scratch flow...
Abstract Background Mesenchymal stem cells (MSCs) exert positive effects in chronic wounds. However, critical parameters, such as the most effective administration routes, remain unclear. Accordingly, purpose of this study was to compare topical and systemic transplantation MSCs on diabetic ischemic wound healing explored underlying mechanisms. Method A model created dorsal foot type 2 diabetes mellitus (T2DM) rat. Bone marrow-derived mesenchymal (BM-MSCs) were administered via two routes:...
Background Diabetic foot disease is a serious complication of diabetes mellitus. Patients with mellitus have 25% lifetime risk for developing ulcer, and between 14% 24% patients require major or minor lower limb amputation due to severe gangrene. However, decisions concerning whether amputate perform amputation, how best determine the plane remain unclear. Methods To consolidate current literature expert opinion make recommendations that will guide surgical diabetic ulcers. A total 23...
AIM: To assess the alterations in resting-state function connections between two cerebral hemispheres patients with optic neuritis (ON) and healthy controls (HCs). METHODS: A total of 12 ON (six males six females) HCs who were highly matched for sex, age, educational level recruited. They underwent functional magnetic resonance imaging (fMRI), testing brain activities assessed using degree centrality (DC) method. Correlation analysis mean DC values specific areas behavior performances was...
To evaluate alterations in conjunctival vascular density (CVD) and macular capillary (MCD) female patients with type 2 diabetes mellitus (T2DM) gestational (GDM) using optical coherence tomography angiography (OCTA). A total of 60 participants were recruited, comprising 20 T2DM, GDM, healthy age-matched controls (HCs). OCTA was used to assess superficial deep retinal plexuses. Subsequently, changes MCD analyzed a circular segmentation method (C1-C6), hemispheric quadrant [superior right...
Abstract This study was conducted to explore the therapeutic potential of human adipose‐derived stem cells (ADSCs) irradiated with a low‐level laser (LLL). Cultured ADSCs were treated 650‐nm GaAlAs irradiation at 2, 4 and 8 J cm −2 . Cell proliferation quantified by MTT assays, cytokine secretion determined enzyme‐linked immunosorbent adipogenic differentiation examined oil red O staining. Additionally, expression profiles putative ADSC surface markers analyzed quantitative real‐time PCR. In...
Background. When vascular endothelial cells are subjected to external stimuli, paracrine hormones and cytokines act on adjacent cells. The regulation of the biological behaviour is closely related maintenance organ function occurrence development disease. However, it unclear whether affect involved in wound repair through autocrine mechanisms ultimately play a role healing. We aimed verify effect functions Materials Methods. ELISA was used detect platelet-derived growth factor, basic...
Acute pancreatitis, a common exocrine inflammatory disease affecting the pancreas, is characterized by intense abdominal pain and multiple organ dysfunction. However, alterations in retinal blood vessels among individuals with acute pancreatitis remain poorly understood. This study employed optical coherence tomography angiography (OCTA) to examine superficial deep patients pancreatitis. Sixteen diagnosed (32 eyes) 16 healthy controls were recruited from First Affiliated Hospital of Nanchang...
Abstract Autologous fat transplantation has been applied widely in clinic. However, the low survival rate is still a problem to be solved. Studies shows that human adipose‐derived stem cells (ADSCs) transfected by vascular endothelial growth factor (VEGF) can improve of autologous transplantation. Our study evaluate effects conditioned medium VEGF‐transfected (VEGF‐ADSCs‐CM) on ADSCs were isolated and with MOI = 40. The was divided into three groups, VEGF‐ADSCs‐CM group, normal‐ADSCs‐CM...
Abstract Background Recent evidence suggests that angiotensin II (Ang ) plays a role in cutaneous wound healing. Mesenchymal stem cells ( MSC s) are known as rich source of re‐establish healed skin. However, the potential impact Ang on differentiation into keratinocytes is still unknown. Objective The present study was conducted to explore effect bone marrow‐derived s BM ‐ keratinocytes. Methods Bone were isolated from rat marrow and cultured. expression type 1 AT 2 receptors examined by...
Adipose‑derived stem cells (ADSCs) are mesenchymal that often used in regenerative medicine. Maintaining ADSC viability is important, as this optimizes the curative effects of cell therapy. However, optimal conditions for preservation remain unknown. The present study aimed to acquire a better protocol storage by comparing various solutions and temperatures preservation, order suggest most effective methods short‑term clinical use. ADSCs from passage 2 were suspended comprising 0.9% NaCl,...
Nonlinear functions are widely used as activation in artificial neural networks, which have a great impact on the fitting ability of networks. Due to complexity function, computation function and its derivative requires lot computing resources time during training. In order improve computational efficiency derivatives back-propagation this paper proposes method based piecewise linear approximation calculate function. This is hardware-friendly universal, it can efficiently compute various...
Clinical treatment of diabetic refractory ulcers is impeded by chronic inflammation and cell dysfunction associated with wound healing. The significant clinical application bFGF in healing limited its instability vivo. Sulfur has been applied for the skin diseases clinic antibiosis. We previously found that sulfur incorporation improves ability selenium nanoparticles to accelerate healing, yet toxicity still poses a risk application. To obtain materials high pro-regeneration activity low...
To study functional brain abnormalities in patients with eye trauma (ET) and to discuss the pathophysiological mechanisms of ET.
Background The present study was conducted to compare 2 purification methods for isolation of human adipose-derived stromal vascular fraction or stem cells (ADSCs) based on red blood cell (RBC) lysis with 155 mM ammonium chloride (NH4Cl) and hypotonic sodium (NaCl) solution, try develop a safe, convenient, cost-effective method clinical applications. Methods Adipose-derived RBC were harvested from the fatty fluid portions liposuction aspirates, respectively. suitable concentration NaCl...