A5090079355- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Malaria Research and Control
- Microbial Metabolic Engineering and Bioproduction
- Lipid metabolism and biosynthesis
- Virology and Viral Diseases
- T-cell and Retrovirus Studies
- COVID-19 epidemiological studies
- Synthesis and biological activity
- Vector-Borne Animal Diseases
- X-ray Diffraction in Crystallography
- Cytomegalovirus and herpesvirus research
- Viral Infections and Immunology Research
- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- Hepatitis B Virus Studies
- Metabolomics and Mass Spectrometry Studies
- Crystallization and Solubility Studies
- Photosynthetic Processes and Mechanisms
- Vibrio bacteria research studies
- Hepatitis Viruses Studies and Epidemiology
- Computational Drug Discovery Methods
- Viral Infections and Outbreaks Research
Purdue University West Lafayette
2013-2023
The recent rapid spread of Zika virus and its unexpected linkage to birth defects an autoimmune neurological syndrome have generated worldwide concern. is a flavivirus like the dengue, yellow fever, West Nile viruses. We present 3.8 angstrom resolution structure mature virus, determined by cryo-electron microscopy (cryo-EM). similar other known structures, except for ~10 amino acids that surround Asn(154) glycosylation site in each 180 envelope glycoproteins make up icosahedral shell....
Abstract The outbreak of Zika virus (ZIKV) in 2016 created worldwide health emergency which demand urgent research efforts on understanding the biology and developing therapeutic strategies. Here, we present a time-resolved chemical proteomic strategy to track early-stage entry ZIKV into host cells. was labeled its surface with probe, carries photocrosslinker covalently link virus-interacting proteins living cells UV exposure at different time points, biotin tag for subsequent enrichment...
Dietary fat absorption by the small intestine is a multistep process that regulates uptake and delivery of essential nutrients energy. One step this temporary storage dietary in cytoplasmic lipid droplets (CLDs). The mobilization thought to be regulated proteins associate with CLD; however, mechanistic details are currently unknown. In study we analyzed proteome CLDs isolated from enterocytes harvested mice following challenge. analysis identified 181 associated CLD fraction, which 37 known...
Cleavage of the flavivirus premembrane (prM) structural protein during maturation can be inefficient. The contribution partially mature virions that retain uncleaved prM to pathogenesis primary infection is unknown. To investigate this question, we characterized functional properties newly-generated dengue virus (DENV) prM-reactive monoclonal antibodies (mAbs) in vitro and using a mouse model DENV disease. Anti-prM mAbs neutralized virion state–dependent manner. Alanine scanning mutagenesis...
Highlights•Zika virus (ZIKV) infects chicken neural progenitors and causes microencephaly•ZIKV strongly certain brain segments or boundaries (signaling centers)•ZIKV induces cell death decreases proliferation within infected regions•Infected signaling centers show reduced morphogen expression abated functionSummaryZika is associated with severe neurodevelopmental impairments in human fetuses, including microencephaly. Previous reports examining progenitor tropism of ZIKV organoid animal...
We report the design and synthesis of a series new 5-chloropyridinyl esters salicylic acid, ibuprofen, indomethacin, related aromatic carboxylic acids for evaluation against SARS-CoV-2 3CL protease enzyme. These ester derivatives were synthesized using EDC in presence DMAP to provide various good excellent yields. Compounds are stable purified by silica gel chromatography characterized 1H-NMR, 13C-NMR, mass spectral analysis. synthetic evaluated our vitro 3CLpro inhibition assay authentic...
Selection and development of monoclonal antibody (mAb) therapeutics against pathogenic viruses depends on certain functional characteristics. Neutralization potency, or the half-maximal inhibitory concentration (IC50) values, is an important characteristic candidate therapeutic antibodies. Structural insights into bases neutralization potency differences between antiviral neutralizing mAbs are lacking. In this report, we present cryo-electron microscopy (EM) reconstructions three...
Aedes aegypti mosquitoes are the primary vectors transmitting dengue virus (DENV), one of most aggressive re-emerging pathogens worldwide causing more than 390 million infections per year. The spread is greatly dependent upon successful replication within both human host and mosquito vector. Much effort has been placed in understanding dynamics transmission organisms, but little known about global impact DENV on metabolic pathways. Previous studies have demonstrated perturbations albopictus...
Cytoplasmic lipid droplets (CLDs) are present and dynamically regulated in enterocytes, the absorptive cells of intestine, response to dietary fat. CLDs have a neutral core surrounded by phospholipid monolayer with associated proteins that diverse cellular functions. The objective this study was determine proteome enterocytes following high fat challenge. We isolated from C57BL6 mice, two hours after 200 μl olive oil gavage. CLD fraction via sucrose gradient ultracentrifugation analyzed HPLC...