A5014406470- Cardiac Fibrosis and Remodeling
- Classical Philosophy and Thought
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Spine and Intervertebral Disc Pathology
- Philosophy, Ethics, and Existentialism
- Cardiovascular Function and Risk Factors
- Nietzsche, Schopenhauer, and Hegel
- Philosophy and Historical Thought
- Electrospun Nanofibers in Biomedical Applications
- Cardiac Structural Anomalies and Repair
- Elasticity and Material Modeling
- Phenomenology and Existential Philosophy
- Cervical and Thoracic Myelopathy
- Protease and Inhibitor Mechanisms
- Cardiac Valve Diseases and Treatments
- Cell Adhesion Molecules Research
- Signaling Pathways in Disease
- Collagen: Extraction and Characterization
- 3D Printing in Biomedical Research
- Tendon Structure and Treatment
- Medieval and Classical Philosophy
- Historical Philosophy and Science
- Philosophical Ethics and Theory
- Heat shock proteins research
University of Arkansas at Fayetteville
2022-2025
Clemson University
2018-2022
Northwestern University
2014-2017
University of Virginia
2013-2016
Duke Medical Center
2014-2016
Duke University Hospital
2014
Duke University
2007-2013
Transnational Press London
2012
University Hospital of North Norway
2007
Baylor University
1959
ABSTRACT Previous study claimed that disc degeneration may be preceded by structure and matrix changes in the intervertebral (IVD) which coincide with loss of distinct notochordally derived nucleus pulposus (NP) cells. However, fate notochordal cells their molecular phenotype change during aging human are still unknown. In this study, a set novel markers NP IVD tissue were revealed immunostaining flow cytometry. Furthermore, potential juvenilization regeneration laminin‐rich pseudo‐3D...
Background Mitral valve prolapse (MVP) is one of the most common forms cardiac disease and affects 2% to 3% population. Previous imaging reports have indicated that myocardial fibrosis in MVP described its association with sudden death. These data combined evidence for postrepair ventricular dysfunction surgical patients support a link between MVP. Methods Results We performed histopathologic analysis left (LV) biopsies from peripapillary regions, inferobasal LV wall apex on MVP, as well...
Aortic valve stenosis (AVS) patients experience pathogenic leaflet stiffening due to excessive extracellular matrix (ECM) remodeling. Numerous microenvironmental cues influence expression of ECM remodeling genes in tissue-resident valvular myofibroblasts, and the regulation complex myofibroblast signaling networks depends on patient-specific factors. Here, we combined a manually curated network with data-driven transcription factor predict gene signatures drug responses. Using transcriptomic...
Matrix metalloproteinases (MMPs) are a family of proteases that drive degradation extracellular matrix (ECM) across many tissues. MMP activity is antagonized by tissue inhibitors (TIMPs), resulting in complex multivariate system with isoforms and TIMP interacting network biochemical reactions - each their own distinct kinetic rates. This complexity makes it very difficult to identify which specific molecules most responsible for driving ECM turnover vivo therefore the promising therapeutic...
Myocardial Infarction (MI) is a major contributor to morbidity and mortality, wherein blood flow blocked portion of the left ventricle leads myocardial necrosis scar formation. Cardiac remodeling in response MI determinant patient prognosis, so many therapies are under development improve infarct healing. Part this involves vitro therapy screening which can be accelerated by engineered heart tissues (EHTs). Unfortunately, EHTs often over-simplify infarcted tissue microarchitecture neglecting...
ABSTRACT The extracellular matrix (ECM) of the human intervertebral disc is rich in molecules that interact with cells through integrin‐mediated attachments. Porcine nucleus pulposus (NP) have been shown to laminin (LM) isoforms LM‐111 and LM‐511 select integrins regulate biosynthesis cell attachment. Since NP lose many phenotypic characteristics age, attachment interaction ECM may be altered. Expression LM‐binding was quantified for using flow cytometry. cell‐ECM mechanism determined by...
Phase II clinical trials have reported that acute treatment of surgical skin wounds with the therapeutic peptide alpha Connexin Carboxy-Terminus 1 (αCT1) improves cutaneous scar appearance by 47% 9-month postsurgery. While Cx43 and ZO-1 been identified as molecular targets αCT1, mode-of-action in mitigation at cellular tissue levels remains to be further characterized. Scar histoarchitecture αCT1 vehicle-control treated within same patient were compared using biopsies from a I trial 29-day...
Regional control of fibrosis after myocardial infarction is critical for maintaining structural integrity in the infarct while preventing collagen accumulation non-infarcted areas. Cardiac fibroblasts modulate matrix turnover response to biochemical and biomechanical cues, but complex interactions between signaling pathways confound efforts develop therapies regional scar formation. We employed a logic-based ordinary differential equation model fibroblast mechano-chemo signal transduction...
Many studies have shown that mechanical forces can alter collagen degradation by proteases, and this mechanochemical effect may potentially serve an important role in determining extracellular matrix content organization load-bearing tissues. However, it is not yet known whether mechano-sensitive depends on particular protease isoforms, nor byproducts be altered loading. In study, we tested the hypothesis different types of proteases exhibit sensitivities to loading both rates byproducts....
Extracellular matrix remodeling after myocardial infarction occurs in a dynamic environment which local mechanical stresses and biochemical signaling species stimulate the accumulation of collagen-rich scar tissue. It is well-known that cardiac fibroblasts regulate post-infarction turnover by secreting proteins, proteases, protease inhibitors response to both stimuli stretch, but how these act together dictate cellular responses still unclear. We developed screen fibroblast-secreted proteins...
Chordoma cells can generate solid-like tumors in xenograft models that express some molecular characteristics of the parent tumor, including positivity for brachyury and cytokeratins. However, there is a dearth markers relate to chordoma tumor growth, as well cell lines needed advance treatment. The objective this study was isolate novel primary source analyze growth mouse model comparison with established U-CH1 U-CH2b lines.Primary from sacral chordoma, called "DVC-4," were cultured...
Arterial hypertension can lead to structural changes within the heart including left ventricular hypertrophy (LVH) and eventually failure with preserved ejection fraction (HFpEF). The initial diagnosis of HFpEF is costly generally based on later stage remodeling; thus, improved predictive diagnostic tools offer potential clinical benefit. Recent work has shown value multibiomarker plasma panels for classification patients LVH HFpEF. We hypothesized that machine learning algorithms could...
The scar that forms after a myocardial infarction is often characterized by highly disordered architecture but generally exhibits some degree of collagen fiber orientation, with resulting mechanical anisotropy. When viewed in finer detail, however, the heterogeneity sample clear, different subregions exhibiting orientations. In this work, we used multiscale finite element model to explore consequences terms behavior. To do so, previously obtained alignment maps rat slices (n = 15) generate...
Abstract Background Cardiac Resynchronization Therapy (CRT) is a widely used, device-based therapy for patients with left ventricle (LV) failure. Unfortunately, many do not benefit from CRT, so there potential value in identifying this group of non-responders before CRT implementation. Past studies suggest that predicting response will require diverse variables, including demographic, biomarker, and LV function data. Accordingly, the objective study was to integrate variable types into...
Autofluorescence (AF) poses challenges for detecting proteins of interest in situ when employing immunofluorescence (IF) microscopy. This interference is particularly pronounced strongly autofluorescent tissues such as myocardium, where tissue AF can be comparable to IF. Although various histochemical methods have been developed achieve effective suppression different types tissue, their applications on myocardial samples not well validated. Due inconsistency across structures sometypes it...
Research documents how spatial location in American metropolitan areas influences individuals' and families' life chances due to differential access opportunity. Racial residential segregation concentrated poverty interact create an especially vulnerable population within cities. Less research has been performed about the detailed perceptions of residents who live spatially isolated neighborhoods with high levels poverty. Using in-depth interviews a Buffalo, New York, neighborhood, we...