A5026869426- Biochemical effects in animals
- Migraine and Headache Studies
- Cannabis and Cannabinoid Research
- Alcohol Consumption and Health Effects
- Barrier Structure and Function Studies
- Neuroscience of respiration and sleep
- S100 Proteins and Annexins
- Adenosine and Purinergic Signaling
- Neuropeptides and Animal Physiology
- Insect and Pesticide Research
- Connexins and lens biology
- RNA modifications and cancer
- Free Radicals and Antioxidants
- Thermal Regulation in Medicine
- Forensic Toxicology and Drug Analysis
- Neonatal and fetal brain pathology
- Amino Acid Enzymes and Metabolism
- Dietary Effects on Health
- Alzheimer's disease research and treatments
- Cerebrospinal fluid and hydrocephalus
- Molecular Biology Techniques and Applications
- CRISPR and Genetic Engineering
- Neuroscience and Neural Engineering
- Heme Oxygenase-1 and Carbon Monoxide
- Receptor Mechanisms and Signaling
University of Arizona
2020-2025
The University of Texas Southwestern Medical Center
2024-2025
Southwestern University
2024
Abstract The endocannabinoid system's significance in maintaining blood–brain barrier (BBB) integrity under physiological and pathological conditions is suggested by several reports, but the underlying molecular mechanisms are not well understood. In this paper, we investigated effects of depletion 2‐arachidonoylglycerol (2‐AG), one main endocannabinoids central nervous system, on BBB using pharmacological tools. Female Sprague–Dawley rats were injected with diacylglycerol lipase α (DAGLα)...
RNA sequencing studies on human dorsal root ganglion (hDRG) from patients suffering neuropathic pain show upregulation of OSM, linking this IL-6 family cytokine to disorders. In mice, however, OSM signaling causes itch behaviors through a direct effect its cognate receptor expressed uniquely by pruriceptive sensory neurons. We hypothesized that an expansion in function OSM-OSM (OSMR) disorders humans could be explained species differences expression and signaling. Our situ hybridization...
Abstract Pharmacological tools for chronic visceral pain management are still limited and inadequate. A 3 adenosine receptor (A AR) agonists effective in different models of persistent pain. Recently, their activity has been related to the block N-type voltage-gated Ca 2+ channels (Ca v 2.2) dorsal root ganglia (DRG) neurons. The present work aimed evaluate efficacy AR reducing postinflammatory hypersensitivity both male female rats. Colitis was induced by intracolonic instillation...
Introduction The high prevalence and severe symptoms of migraines in humans emphasizes the need to identify underlying mechanisms that can be targeted for therapeutic benefit. Clinical Endocannabinoid Deficiency (CED) posits reduced endocannabinoid tone may contribute migraine development other neuropathic pain conditions. While strategies increase levels n-arachidonoylethanolamide have been tested, few studies investigated targeting more abundant endocannabinoid, 2-arachidonoylgycerol, as...
Disruption of blood-brain barrier integrity and dramatic failure brain ion homeostasis including fluctuations pH occurs during cortical spreading depression (CSD) events associated with several neurological disorders, migraine aura, traumatic injury stroke. NHE1 is the primary regulator in central nervous system. The goal current study was to investigate role sodium-hydrogen exchanger type 1 (NHE1) blood (BBB) CSD contributions this antiporter on xenobiotic uptake. Using immortalized cell...
Abstract CRISPR-Cas9 editing is now the leading method for genome and being advanced treatment of human disease. CRIPSR could have many applications neurological diseases, including pain but traditional viral vector delivery approaches neurotoxicity limiting their use. Overcoming these issues open door treatments diseases like intractable where dorsal root ganglia (DRG) would be desired target. To this end, we describe a simple viral-vector-independent transfection primary DRG (hDRG) neurons...
Pathologies of the blood brain barrier (BBB) have been linked to a multitude CNS disorders whose pathology is poorly understood. Cortical spreading depression (CSD) has long postulated be involved in underlying mechanisms these disease states, yet full understanding remains elusive. This study utilized an vitro model BBB with b.End3 murine endothelial cells investigate role CSD by characterizing effects release major pronociceptive substances on functional integrity using TEER screening,...
Cortical spreading depression (CSD) is a pathophysiological mechanism underlying headache disorders, including migraine. Blood-brain barrier (BBB) permeability increased during CSD. Recent papers have suggested that heat shock proteins (HSP) contribute to the integrity of blood-brain barrier. In this study, possible role HSP90 in CSD-associated leak at endothelial cell was investigated using an vitro model, for blood-endothelial (BEB), and vivo model with intact BBB. We measured trans...
Protocol for generating suspensions of fixed human nuclei single-nucleus transcriptomics.
Protocol for generating suspensions of fixed neuronal nuclei (NeuN+) from human central nervous system tissue single-nucleus transcriptomics.
Background: Mounting evidence supports the role of endocannabinoid system in neurophysiology, includ-ing blood-brain barrier (BBB) function. Recent work has demonstrated that activation re-ceptors can mitigate insults to BBB during neurological disorders, like traumatic brain injury, cortical spreading depression and stroke. As alterations are associated with worsening clinical outcomes these conditions, studies herein sought examine impact depletion on integrity. Methods: Barrier integrity...
Mounting evidence supports the role of endocannabinoid system in neurophysiology, including blood-brain barrier (BBB) function. Recent work has demonstrated that activation receptors can mitigate insults to BBB during neurological disorders like traumatic brain injury, cortical spreading depression, and stroke. As alterations are associated with worsening clinical outcomes these conditions, studies herein sought examine impact depletion on integrity. Barrier integrity was investigated vitro...
Pathologies of the blood-brain barrier (BBB) have been linked to a multitude central nervous system (CNS) disorders whose pathology is poorly understood. Cortical spreading depression (CSD) has long postulated be involved in underlying mechanisms these disease states, yet complete understanding remains elusive. This study seeks utilize an vitro model with brain endothelial cell (b.End3) murine endothelioma cells investigate role CSD BBB by characterizing effects release major pronociceptive...
Cortical spreading depression (CSD) is a pathophysiological mechanism underlying headache disorders, including migraine. Blood-brain barrier (BBB) permeability increased during CSD. Recent papers have suggested that heat-shock proteins (HSP) contribute to the integrity of blood-brain barrier. In this study, possible role HSP90 in CSD-associated leak at endothelial cell was investigated using an vitro model, for blood (BEB), and vivo model with intact BBB. We measured trans electric...
This study examines the roles of 2-AG hydrolyzing enzymes MAGL and ABHD6 as they pertain to headache development within KCl model migraine. We seek further our understanding how modulation endocannabinoid system may underlie migraine development.