A5015203512- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Phosphodiesterase function and regulation
- Memory and Neural Mechanisms
- Sleep and Wakefulness Research
- Endoplasmic Reticulum Stress and Disease
- RNA Research and Splicing
- Microbial Metabolic Engineering and Bioproduction
- Ion channel regulation and function
- Protist diversity and phylogeny
- Biochemical and Molecular Research
- RNA regulation and disease
- Genetics and Neurodevelopmental Disorders
- Enzyme Structure and Function
- Mitochondrial Function and Pathology
- Photosynthetic Processes and Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Retinal Development and Disorders
- Glycosylation and Glycoproteins Research
- Microtubule and mitosis dynamics
- Peroxisome Proliferator-Activated Receptors
University of California, San Diego
2018-2024
Neurocrine Biosciences (United States)
2024
University of Pennsylvania
2012-2017
Rensselaer Polytechnic Institute
2010
Brief periods of sleep loss have long-lasting consequences such as impaired memory consolidation. Structural changes in synaptic connectivity been proposed a substrate storage. Here, we examine the impact brief deprivation on dendritic structure. In mice, find that five hours decreases spine numbers selectively hippocampal area CA1 and increased activity filamentous actin severing protein cofilin. Recovery normalizes these structural alterations. Suppression cofilin function prevents loss,...
A kinase-anchoring proteins (AKAPs) organize compartmentalized pools of protein kinase (PKA) to enable localized signaling events within neurons. However, it is unclear which the many expressed AKAPs in neurons target PKA complexes important for long-lasting forms synaptic plasticity and memory storage. In forebrain, anchoring gravin recruits a complex containing PKA, PKC, calmodulin, PDE4D (phosphodiesterase 4D) β2-adrenergic receptor. Here, we show that mice lacking α-isoform have deficits...
Long-lasting forms of long-term potentiation (LTP) represent one the major cellular mechanisms underlying learning and memory. One fundamental questions in field LTP is why different molecules are critical for long-lasting induced by diverse experimental protocols. Further complexity stems from spatial aspects signaling networks, such that some function dendrite spine. We investigated whether evidence can be unified creating a spatial, mechanistic model multiple pathways hippocampal CA1...
Hyperpolarization‐activated and cyclic nucleotide‐gated (HCN) channels mediate the I h current in murine hippocampus. Disruption of by knockout HCN1, HCN2 or tetratricopeptide repeat‐containing Rab8b‐interacting protein has been shown to affect physiological processes such as synaptic integration maintenance resting membrane potentials well several behaviors mice, including depressive‐like anxiety‐like behaviors. However, potential involvement HCN4 isoform these is unknown. Here, we assessed...
Genetic manipulation of cells and tissue by RNA interference has significantly contributed to the functional characterization individual proteins their role in physiological processes. Despite its versatility, can have detrimental side effects, including reduced cell viability. We applied recombinant adeno-associated viruses stereotaxic injection into murine hippocampus express different short hairpin (shRNA) constructs along with eGFP. Tissue responses were assessed immunohistochemically...
SUMMARY Glucose, the primary cellular energy source, is metabolized through glycolysis initiated by rate-limiting enzyme Hexokinase (HK). In energy-demanding tissues like brain, HK1 dominant isoform, primarily localized on mitochondria, crucial for efficient glycolysis-oxidative phosphorylation coupling and optimal generation. This study unveils a unique mechanism regulating activity, glycolysis, dynamics of mitochondrial coupling, mediated metabolic sensor O-GlcNAc transferase (OGT). OGT...