A5030854033- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- COVID-19 diagnosis using AI
- Child Nutrition and Water Access
- Malaria Research and Control
- Viral Infections and Outbreaks Research
- Clinical Nutrition and Gastroenterology
- Lymphatic System and Diseases
- Nutrition and Health in Aging
- Childhood Cancer Survivors' Quality of Life
- Complement system in diseases
- Neonatal Respiratory Health Research
- HIV-related health complications and treatments
- vaccines and immunoinformatics approaches
- Poverty, Education, and Child Welfare
- HIV Research and Treatment
- Biosensors and Analytical Detection
- Neonatal and fetal brain pathology
- Parasites and Host Interactions
- Infant Health and Development
- Fetal and Pediatric Neurological Disorders
- Invertebrate Immune Response Mechanisms
- Scientific Computing and Data Management
- Simulation Techniques and Applications
- Viral gastroenteritis research and epidemiology
Wellcome Sanger Institute
2025
Kenya Medical Research Institute
2019-2024
Wellcome Trust
2020-2023
The Childhood Acute Illness and Nutrition Network
2019-2022
Children admitted to hospital with an acute illness and concurrent severe malnutrition [complicated (CSM)] have a high risk of dying. The biological processes underlying their mortality are poorly understood. In this case-control study nested within multicenter randomized controlled trial among children CSM in Kenya Malawi, we found that blood metabolomic proteomic profiles robustly differentiated who died ( n = 92) from those survived 92). Fatalities were characterized by increased...
Abstract Background Cumulative malaria parasite exposure in endemic regions often results the acquisition of partial immunity and asymptomatic infections. There is limited information on how host-parasite interactions mediate maintenance chronic symptomless infections that sustain transmission. Methods Here, we determined gene expression profiles population corresponding host peripheral blood mononuclear cells (PBMCs) from 21 children (< 15 years). We compared who were defined as...
<ns4:p><ns4:bold>Background.</ns4:bold> International recommendations for the control of coronavirus disease 2019 (COVID-19) pandemic emphasize central role laboratory testing severe acute respiratory syndrome 2 (SARS-CoV-2), etiological agent, at scale. The availability reagents, equipment and qualified staff are important bottlenecks to achieving this. Elsewhere, pooled (i.e. combining multiple samples in same reaction) has been suggested increase capacities period.</ns4:p><ns4:p>...
Background. International recommendations for the control of coronavirus disease 2019 (COVID-19) pandemic emphasize central role laboratory testing severe acute respiratory syndrome 2 (SARS-CoV-2), etiological agent, at scale. The availability reagents, equipment and qualified staff are important bottlenecks to achieving this. Elsewhere, pooled (i.e. combining multiple samples in same reaction) has been suggested increase capacities period. Methods. We discuss our experience with SARS-CoV-2...
<ns4:p><ns4:bold>Background:</ns4:bold> The global COVID-19 outbreak relies on a quantitative real-time polymerase chain reaction (qRT-PCR) for the detection of severe acute respiratory syndrome coronavirus (SARS-CoV-2), to facilitate roll-out patient care and infection control measures. There are several qRT-PCR assays with little evidence their comparability. We report alterations developers’ recommendations sustain testing capability in our setting, where supply reagents is...
<ns4:p><ns4:bold>Introduction</ns4:bold>: Many acutely ill children in low- and middle-income settings have a high risk of mortality both during after hospitalisation despite guideline-based care. Understanding the biological mechanisms underpinning may suggest optimal pathways to target for interventions further reduce mortality. The Childhood Acute Illness Nutrition (CHAIN) Network (<ns4:ext-link xmlns:ns5="http://www.w3.org/1999/xlink" ext-link-type="uri"...
Abstract HIV infection affects up to 30% of children presenting with severe acute malnutrition (SAM) in Africa and is associated increased mortality. Children SAM are treated similarly regardless status, although mechanisms nutritional recovery and/or not well understood. We performed a secondary analysis clinical trial plasma proteomics data among complicated Kenya Malawi. Compared without (n = 113), HIV-infected 54) had evidence (false discovery rate (FDR) corrected p < 0.05) metabolic...
<ns4:p><ns4:bold>Background:</ns4:bold> The COVID-19 pandemic relies on real-time polymerase chain reaction (qRT-PCR) for the detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), to facilitate roll-out patient care and infection control measures. There are several qRT-PCR assays with little evidence their comparability. We report alterations developers’ recommendations sustain testing capability in a resource-limited setting.</ns4:p><ns4:p> <ns4:bold>Methods:</ns4:bold>...
<ns4:p><ns4:bold>Introduction</ns4:bold>: Many acutely ill children in low- and middle-income settings have a high risk of mortality both during after hospitalisation despite guideline-based care. Understanding the biological mechanisms underpinning may suggest optimal pathways to target for interventions further reduce mortality. The Childhood Acute Illness Nutrition (CHAIN) Network (<ns4:ext-link xmlns:ns5="http://www.w3.org/1999/xlink" ext-link-type="uri"...
<ns4:p><ns4:bold>Background:</ns4:bold> <ns4:italic>Plasmodium falciparum</ns4:italic> causes the deadliest form of malaria in humans. Upon infection, host’s infected red blood cells (iRBCs) are remodelled by exported parasite proteins order to provide a niche for development and maturation.</ns4:p><ns4:p> <ns4:bold>Methods:</ns4:bold> Here we analysed role three PHISTb Pf3D7_0532400, Pf3D7_1401600, Pf3D7_1102500 expressing recombinant evaluated antibody responses against these using immune...
Abstract The syndrome of kwashiorkor is a striking phenotype childhood severe malnutrition (SM) comprising oedema, fatty liver, and skin hair changes. Despite high fatality, the aetiology pathophysiology remain enigmatic, including role serum albumin on oedema development. Here, we demonstrate that associated with presence severity among severely malnourished children. Further, in two independent cohorts children Malawi Kenya, show albumin-independent mechanisms are SM, oxidative stress...
<ns4:p><ns4:bold>Background:</ns4:bold> <ns4:italic>Plasmodium falciparum</ns4:italic> causes the deadliest form of malaria in humans. Upon infection, host’s infected red blood cells (iRBCs) are remodelled by exported parasite proteins to provide a niche for development and maturation.</ns4:p><ns4:p> <ns4:bold>Methods:</ns4:bold> Here we analysed role three PHISTb Pf3D7_0532400, Pf3D7_1401600, Pf3D7_1102500 expressing recombinant evaluated antibody responses against these using immune sera...
Abstract Cumulative malaria parasite exposure in endemic regions often results the acquisition of partial immunity and asymptomatic infections. There is limited information on how host-parasite interactions mediate maintenance chronic symptomless infections that sustain transmission. Here, we have determined gene expression profiles population corresponding host peripheral blood mononuclear cells (PBMCs) from 21 children (< 15 years). We compared who were defined as uninfected, those with...
Abstract HIV infection affects up to 30% of children presenting with severe acute malnutrition (SAM) in Africa and is associated increased mortality. Children SAM are treated similarly regardless status, although mechanisms nutritional recovery and/or not well understood. We performed a secondary analysis clinical trial plasma proteomics data among complicated Kenya Malawi. Compared without (n = 113), HIV-infected 54) had evidence (false discovery rate (FDR) corrected p<0.05) metabolic...