A5062786745- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Genomics and Phylogenetic Studies
- Infective Endocarditis Diagnosis and Management
- Enterobacteriaceae and Cronobacter Research
- Microbial infections and disease research
- Complement system in diseases
- Influenza Virus Research Studies
- Reproductive tract infections research
- Pneumocystis jirovecii pneumonia detection and treatment
- Virology and Viral Diseases
- Respiratory viral infections research
- Antibiotic Resistance in Bacteria
- Peptidase Inhibition and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Cystic Fibrosis Research Advances
- Bacterial Identification and Susceptibility Testing
- RNA and protein synthesis mechanisms
- Computational Drug Discovery Methods
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
National Center for Immunization and Respiratory Diseases
2017-2024
Centers for Disease Control and Prevention
2018-2024
CDC Foundation
2019-2024
Office of Infectious Diseases
2018
Georgia Institute of Technology
2017
Use of eculizumab (Soliris, Alexion Pharmaceuticals), a terminal complement inhibitor, is associated with 1,000-fold to 2,000-fold increased incidence meningococcal disease (1). Administration vaccines recommended for patients receiving before beginning treatment (2,3). Sixteen cases were identified in recipients the United States during 2008-2016; among these, 11 caused by nongroupable Neisseria meningitidis. Fourteen had documentation receipt at least 1 dose vaccine onset. Because remain...
This article reviews the risk of meningococcal disease following eculizumab treatment in patients who have received vaccine. Early Release/July 7, 2017/66. Use (Soliris, Alexion Pharmaceuticals), a terminal complement inhibitor, is associated with 1000-fold to 2000-fold increased incidence (1Food and Drug Administration. briefing information for November 18, 2014, meeting Safety Risk Management Advisory Committee....
Gonorrhea, a sexually transmitted disease, causes substantial global morbidity and economic burden. New prevention control measures for this disease are urgently needed, as strains resistant to almost all classes of antibiotics available treatment have emerged. Previous reports demonstrate that cross-protection from gonococcal infections may be conferred by meningococcal serogroup B (MenB) outer membrane vesicle (OMV)-based vaccines. Among 1,525 common proteins shared across the genomes both...
Abstract Background Haemophilus influenzae (Hi) can cause invasive diseases such as meningitis, pneumonia, or sepsis. Typeable Hi includes six serotypes (a through f), each expressing a unique capsular polysaccharide. The capsule, encoded by the genes within capsule locus, is major virulence factor of typeable Hi. Non-typeable (NTHi) does not express and associated with non-invasive diseases. Methods A total 395 293 NTHi isolates were characterized whole genome sequencing (WGS). Phylogenetic...
Invasive meningococcal disease is mainly caused by Neisseria meningitidis serogroups A, B, C, X, W, and Y. The serogroup typically determined slide agglutination serogrouping (SASG) real-time PCR (RT-PCR).
Historically, the major cause of meningococcal epidemics in meningitis belt sub-Saharan Africa has been Neisseria meningitidis serogroup A (NmA), but incidence substantially reduced since introduction a conjugate vaccine starting 2010. We performed whole-genome sequencing on isolates collected post-2010 to assess their phylogenetic relationships and inter-country transmission. total 716 invasive between 2011 2016 from 11 countries were sequenced for molecular characterization by three WHO...
Recent reports have indicated a rise of invasive disease caused by Haemophilus influenzae serotype (Hia) in North America and some European countries. The whole-genome sequences for total 410 Hia isolates were obtained from 12 countries spanning the years 1998 to 2019 underwent phylogenetic comparative genomic analysis order characterize major strains causing genetic variation present among factors contributing virulence antimicrobial resistance. Among isolate received, 408 passed our...
Predictions of vaccine efficacy against Neisseria meningitidis serogroup B (NmB) disease are hindered by antigenic variability, limiting the representativeness individual NmB isolates. A qualitative human serum bactericidal assay using endogenous complements subjects (enc-hSBA) enables large panels isolates to be tested. 110-isolate panel was randomly selected from 442 invasive United States cases reported Centers for Disease Control (CDC) 2000 2008. Typing analyses confirmed is...
Bacterial meningitis is a life-threatening infection that remains public health concern. commonly caused by the following species: Neisseria meningitidis, Streptococcus pneumoniae, Listeria monocytogenes, Haemophilus influenzae and Escherichia coli. Here, we describe BMScan (Bacterial Meningitis Scan), whole-genome analysis tool for species identification of bacterial meningitis-causing closely-related pathogens, an essential step case management disease surveillance. relies on reference...
In the first 2 years after a nationwide mass vaccination campaign of 1-29-year-olds with meningococcal serogroup A conjugate vaccine (MenAfriVac) in Burkina Faso, carriage and disease due to Neisseria meningitidis were nearly eliminated. We aimed assess long-term effect MenAfriVac on herd immunity.We did four cross-sectional studies people aged 9 months 36 two districts Faso between May 2, 2016, Nov 6, 2017. Demographic information oropharyngeal swabs collected. Meningococcal isolates...
We characterized 22 meningococcal disease cases due to nongroupable Neisseria meningitidis, a rare cause of invasive disease. Disease presentation and severity were similar those for serogroupable However, 7 (32%) patients had complement deficiency or abnormal testing results, highlighting the importance cases.
To monitor the burden and changes in Haemophilus influenzae (Hi) disease, direct real-time PCR (drt-PCR) assays have been developed for Hi detection monoplex form its six serotypes triplex form, directly from cerebrospinal fluid (CSF) specimens. These target phoB gene species (Hi-phoB) serotype-specific genes region II of capsule biosynthesis locus (Hi-abf Hi-cde), identified through comparative analysis non-Hi whole-genome sequences. The lower limit (LLD) is 293 CFU/mL Hi-phoB assay ranged...
Abstract Rollout of meningococcal serogroup A conjugate vaccine in Africa started 2010, aiming to eliminate meningitis outbreaks, belt countries. Since then, studies have been conducted, primarily using isolates, assess the impact on distribution strains region. Here, we implemented an innovative, culture-free whole-genome sequencing approach almost 400 clinical specimens collected between 2017 and 2019 from cases 6 African About 50% provided high-quality sequence data for comprehensive...
Effective laboratory-based surveillance and public health response to bacterial meningitis depends on timely characterization of pathogens. Traditionally, characterizing pathogens such as Neisseria meningitidis (Nm) Haemophilus influenzae (Hi) required several biochemical molecular tests. Whole genome sequencing (WGS) has enabled the development pipelines capable given pathogen with equivalent results many traditional Here, we present Bacterial Meningitis Genomic Analysis Platform (BMGAP): a...
In 2015 and 2016, meningococcal carriage evaluations were conducted at two universities in the United States following mass vaccination campaigns response to Neisseria meningitidis serogroup B (NmB) disease outbreaks. A simultaneous evaluation was also a university near one of outbreaks, where no NmB cases reported occurred. total ten cross-sectional rounds conducted, resulting 1,514 isolates collected from 7,001 unique participants; 1,587 individuals swabbed multiple time points (repeat...
In January and February 2015, Neisseria meningitidis serogroup B (NmB) outbreaks occurred at two universities in the United States, mass vaccination campaigns using MenB vaccines were initiated as part of a public health response. Meningococcal carriage evaluations conducted concurrently with these third university, where no NmB outbreak occurred. isolates (N = 1,514) obtained from characterized for capsule biosynthesis by whole-genome sequencing (WGS). Functional polysaccharide synthesis...
Background: In 2019, 5 cases of invasive Haemophilus influenzae serotype b (Hib) occurred in the Anchorage region Alaska over a period 16 days. No had preceding 26 months. Methods: Hib isolates from 2005 through 2019 were analyzed using whole-genome sequencing (WGS). Rates compared with CDC’s Active Bacterial Core surveillance (ABCs) data. Results: A total 33 2019. Of associated cluster, 2 (40%) adults and all region. contrast, only 14% (4/28) noncluster this ( P < 0.01). Two cluster...
http://rampdb.biology.gatech.edu.
The impact of serogroup B meningococcal (MenB) vaccines on carriage is not completely understood. Using whole-genome sequencing data, we assessed the diversity and distribution MenB vaccine antigens (particularly FHbp) among 1,514 isolates recovered from vaccinated unvaccinated students at three U.S. universities, two which underwent MenB-FHbp mass vaccination campaigns following disease outbreaks.
Togo has reported seasonal meningitis outbreaks caused by non-Neisseria meningitidis serogroup A (NmA) pathogens since the introduction of meningococcal conjugate vaccine (MACV, MenAfriVac) in 2014. From 2016 to 2017, NmW several outbreaks. In early 2019, a NmC outbreak was detected Savanes region and its investigation is described here. Under case-based surveillance, epidemiological clinical data, cerebrospinal fluid specimens were collected for every suspected case meningitis. Specimens...