A5026293971- Hepatitis C virus research
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Animal Virus Infections Studies
- vaccines and immunoinformatics approaches
- Viral Infections and Immunology Research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Hepatitis Viruses Studies and Epidemiology
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Immunodeficiency and Autoimmune Disorders
- Viral Infections and Outbreaks Research
- T-cell and B-cell Immunology
- Analytical Chemistry and Chromatography
- Tuberculosis Research and Epidemiology
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Systemic Lupus Erythematosus Research
- Glycosylation and Glycoproteins Research
- Immune cells in cancer
Transgene (France)
2012-2023
bioMérieux (France)
2001-2016
Centre National de la Recherche Scientifique
1984-2016
Inserm
1996-2013
Saint Louis University
2013
Virginia Mason Medical Center
2013
Hôpital de la Croix-Rousse
2010
Hospices Civils de Lyon
2010
Université de Lyon
2010
Université Claude Bernard Lyon 1
2010
Genomic RNA from the human prototype strain H of hepatitis C virus (HCV-H) has been molecularly cloned and sequenced. The HCV-H sequence reported consists 9416 nucleotides including 5' 3' untranslated regions. shows 96% amino acid identity with American isolate HCV-1 but only 84.9% Japanese isolates HCV-J HCV-BK. In addition to hypervariable region (region V) previously identified in putative E2 domain, three other variable domains were identified: V1 (putative E1), V2 E2), V3 NS5). These...
The presence of hepatitis C virus (HCV) negative strand RNA in extrahepatic compartments based on PCR detection assays has been suggested many reports with a very heterologous rate (from 0 to 100%). In this study, we have analyzed the HCV hepatic (liver biopsies, n = 20) and (sera, 32; PBMC, 26 fresh bone marrow cells, 8) from infected patients three different reverse transcriptase (RT)-PCR-based using primers located 5' noncoding region, or without tag selected display viral loads (10(5)-3...
To investigate which hairpin structures within the 5′ untranslated region of hepatitis C virus (HCV) are necessary for cap‐independent translation, mutants were constructed that lack one or more structures. Here we demonstrate, by constructing precisely defined deletion mutants, with exception most located structure, on shows an increase each predicted hairpins is found to be essential translation. In addition, demonstrate HCV 5′UTR driven translation stimulated poliovirus 2A pro co‐expression.
Vectors expressing the first 58 amino acids of hepatitis C virus (HCV) nucleocapsid alone or as a fusion protein with middle (pre-S2 and S) major (S) surface antigens B (HBV) were constructed. Intramuscular immunization BALB/c mice chimeric constructs in form naked DNA elicited humoral responses to from both viruses within 2 6 weeks postinjection. No anti-HCV obtained immunized vector HCV sequence nonfusion context. Sera chimera-injected specifically recognized capsid HBV enzyme-linked...
We have isolated cDNA clones from the 5' end of Hutchinson strain hepatitis C virus. Sequences encoding various segments HCV structural region were fused to gene for glutathione S-transferase and analyzed expression virus-capsid fusion proteins. With a set these proteins, both human chimpanzee immune responses capsid studied. An immunodominant epitope was located within amino-terminal portion that is preferentially recognized by antibodies in virus-positive sera. In addition, analyses...
<h3>Objective</h3> To assess a new adenovirus-based immunotherapy as novel treatment approach to chronic hepatitis B (CHB). <h3>Methods</h3> TG1050 is non-replicative adenovirus serotype 5 encoding unique large fusion protein composed of truncated HBV Core, modified Polymerase and two Envelope domains. We used recently described HBV-persistent mouse model based on recombinant adenovirus-associated virus an over length genome that induces the production HBsAg, HBeAg infectious particles...
Treatment of chronic hepatitis B (CHB) typically requires life-long administration drugs. Cohort and pre-clinical studies have established the link between a functional T-cell-mounted immunity resolution infection. TG1050 is an adenovirus 5-based vaccine that expresses HBV polymerase domains core surface antigen has shown immunogenicity antiviral effects in mice. We performed phase 1 clinical trial to assess safety explore early efficacy CHB patients. This randomized, double blind,...
In a murine model, we have compared humoral and T-helper (Th) responses induced following genetic immunization with two hepatitis C virus (HCV) plasmid-derived immunogens: plasmid expressing the fulllength nucleocapsid (CAP) as nonsecreted antigen (pCMVC2) amino-terminal part of CAP secreted (pS2S.C2N). BALB/c mice, intramuscular injection either IgG2a antibodies associated Thl-like profile characterized by in vitro splenic production interleukin-2 (IL-2) interferon-γ (IFN-γ). The pS2S.C2N...
Abstract Broad T cell and B responses to multiple HCV antigens are observed early in individuals who control or clear infection. The prevailing hypothesis has been that similar immune induced by prophylactic immunization would reduce acute virus replication protect exposed from chronic Here, we demonstrate of naïve chimpanzees with a multicomponent vaccine robust HCV-specific responses, all vaccinees heterologous chimpanzee-adapted 1b J4 significantly reduced viral RNA serum 84%, liver 99%...
The relationship between hepatitis C virus RNA and virus-associated antibodies (antibody against the putative capsid protein C-100 antibody) was determined by nested polymerase chain reaction enzyme-linked immunosorbent assay in serial serum samples obtained from eight chimpanzees experimentally infected with virus. Three different patterns emerged data: first (group 1) acute resolving transient appearance of HCV (two cases). second 2) had chronic persistent positivity (four cases) third 3)...
Primary human hepatocytes were used to develop a culture model for in vitro propagation of hepatitis C virus (HCV). Production positive- strand full-length viral RNA cells and supernatants was monitored by PCR methods targeting three regions the genome: 5' NCR, 3' X-tail envelope glycoprotein E2. De novo synthesis negative-strand also demonstrated. Evidence gradual increase components over 3 month period obtained two quantitative assays: one evaluation genomic titre (quantitative PCR)...
Prophylactic hepatitis C virus (HCV) vaccine trials with human volunteers are pending. There is an important need for immunological end points which correlate efficacy and do not involve invasive procedures, such as liver biopsies. By using a multicomponent DNA priming-protein boosting strategy, naïve chimpanzees were immunized against HCV structural proteins (core, E1, E2) well nonstructural (NS3) protein. Following immunization, exposure to the heterologous 1b J4 subtype resulted in peak...
Vero cells were cotransfected with pSV2neo and a recombinant plasmid containing the varicella-zoster virus (VZV) open reading frame 62 (ORF62). Three neomycin-resistant cell lines isolated shown to complement two different ICP4 mutants (tsB21 d120) of herpes simplex (HSV) type 1 (HSV-1). VZV-specific RNA could not be detected in these lines, but following infection tsB21, 4.3-kilobase VZV transcript was detected. This increased quantity when infected presence cycloheximide. A protein 175...
Three oligonucleotide primer combinations selected from the 5′ noncoding, nucleocapsid and putative nonstructural regions of hepatitis C virus genome were compared in a nested polymerase chain reaction assay with respect to sensitivity specificity for detection viral RNA chimpanzeeinfected human-infected sera. Sera both acute chronic phase infection obtained 13 animals inoculated five different non-A, non-B strains seven cardiac surgery patients who had develop after transfusion been tested...
Hepatitis C virus (HCV) is a leading cause of chronic viral hepatitis worldwide. The study antibody-mediated neutralization has been hampered by the lack an efficient and high-throughput cell culture system for neutralization. HCV structural proteins have shown to assemble into noninfectious HCV-like particles (HCV-LPs). Similar serum-derived virions, HCV-LPs bind enter human hepatocytes hepatoma lines. In this study, we developed HCV-LP-based model systematic functional analysis antiviral...