- Plant Stress Responses and Tolerance
- Plant Genetic and Mutation Studies
- Wheat and Barley Genetics and Pathology
- Heat shock proteins research
- Aluminum toxicity and tolerance in plants and animals
- Fermentation and Sensory Analysis
- Heavy metals in environment
- Meat and Animal Product Quality
- Horticultural and Viticultural Research
- Genetics and Plant Breeding
- Crop Yield and Soil Fertility
- Plant-Microbe Interactions and Immunity
- Fungal Biology and Applications
- Plant biochemistry and biosynthesis
- Biosensors and Analytical Detection
- Plant responses to elevated CO2
- Polysaccharides and Plant Cell Walls
- Enzyme-mediated dye degradation
- Advanced biosensing and bioanalysis techniques
- bioluminescence and chemiluminescence research
- Microbial Metabolism and Applications
- Wine Industry and Tourism
- Plant Molecular Biology Research
- Yeasts and Rust Fungi Studies
- Plant Gene Expression Analysis
University of Salento
2015-2024
Istituto Nazionale di Fisica Nucleare, Sezione di Lecce
1997-2007
Istituto Sperimentale per la Zootecnia
1999
ABSTRACT Water deficit is a severe environmental stress and the major constraint on plant productivity with an evident effect growth. The aim of this work was to study Triticum Aegilops seedlings differing in their response drought at physiological molecular levels. identification resistant sensitive genotypes firstly based relative water content (RWC) measurement. Further characterization contrasting performed level by determination RWC, loss rate (WLR) free proline after different hours...
Durum wheat often faces water scarcity and high temperatures, two events that usually occur simultaneously in the fields. Here we report on stress responsive strategy of durum cultivars, characterized by different use efficiency, subjected to drought, heat a combination both stresses. The cv Ofanto (lower efficiency) activated large set well-known drought-related genes after drought treatment, while Cappelli (higher showed constitutive expression several induced modulation limited number...
Saccharomyces cerevisiae is the yeast species predominating alcoholic fermentation of grape must. The aim this research was to evaluate impact indigenous S. strains biodiversity on aroma wines from Negroamaro grapes. Grapes collected in two different producing micro districts Salento (Southern Italy), were subjected natural and populations isolated. Fifteen for each selected tested by assay order their specific contribute volatiles composition sensory produced wines. aromatic profile...
Heavy metal excess in soil represents a critical problem for crop productivity. Among these pollutants, cadmium (Cd) is one of the most dangerous terms food-chain contamination. Two durum wheat near-isogenic lines (NILs) and 12 commercial varieties (cultivars Arcangelo, Aureo, Aziziah, Cappelli, Cirillo, Creso, Iride, Maestrale, Parsifal, Russello, Strongfield, Svevo) were exposed to nontoxic level Cd evaluate its concentration grains, roots, shoots, as well effects on biomass production....
Among cereals, durum wheat (Triticum turgidum L. subsp. durum) accumulates cadmium (Cd) at higher concentration if grown in Cd-polluted soils. Since accumulation is a risk for human health, the international trade organizations have limited acceptable of Cd edible crops. Therefore, cultivars accumulating low grains should be preferred by farmers and consumers. To identify response to presence Cd, transcriptomes roots shoots Creso Svevo were sequenced after 50-day exposure 0.5 μM hydroponic...
Due to global climate change, complex combinations of stresses are expected occur, among which the interaction between pathogens and drought stress may have a significant effect on growth yield. In this study, Xylella fastidiosa (Xf)-resistant cultivar Leccino susceptible one Cellina di Nardò were subjected (a) individual stress, (b) Xf infection (c) combination both conditions. Here we report physiological response in water content leaves modulation expression level seven genes responsive...
Heat and drought stress have emerged as major constraints for durum wheat production. In the Mediterranean area, their negative effect on crop productivity is expected to be exacerbated by occurring climate change. Xyloglucan endotransglucosylase/hydrolases (XTHs) are chief enzymes in cell wall remodelling, whose relevance expansion morphogenesis suggests a central role responses. this work potential of XTHs abiotic tolerance was investigated wheat. The separate effects dehydration heat...
The ERY4 laccase gene of Pleurotus eryngii is not biologically active when expressed in yeast. To explain this finding, we analysed the role C-terminus Ery4 protein by producing a number its different mutant variants. Two categories genes were produced and yeast: (i) mutants carrying C-terminal deletions (ii) site-specific mutations at their C-terminus. Investigation catalytic properties recombinant enzymes indicated that each novel variant acquired affinities activity for various...
Drought causes massive crop quality and yield losses. Limiting the adverse effects of water deficits on is an urgent goal for a more sustainable agriculture. With this aim, six chicory varieties were subjected to drought conditions during seed germination at week-old plant growth stage, in order identify some morphological and/or molecular markers resistance. Selvatica, Zuccherina di Trieste Galatina varieties, with high vegetative development, showed major index, greater seedling...
Heat shock proteins play an essential role in preventing deleterious effects of high temperatures. In many plants, HSP101 has a central heat stress survival. We report the isolation and characterization four cDNAs corresponding to different members durum wheat gene family. Expression analysis revealed differences their induction. Accordingly, genes are differently regulated, therefore having distinct roles response thermotolerance acquisition. These findings important for further dissection...
A yeast strain was isolated during a study on vineyard-associated strains from Apulia in Southern Italy. ITS and LSU D1/D2 rDNA sequences showed this not to belong any known species described as the type of Ogataea uvarum sp. nov., close relative O. philodendri. Several secondary peaks appeared sequences, suggesting internal heterogeneity among copies rDNA. This hypothesis tested by sequencing single clones marker region. The analyses different levels variability throughout operon with...