A5034878518- Plant Reproductive Biology
- Nitrogen and Sulfur Effects on Brassica
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Genetic diversity and population structure
- Education, Psychology, and Social Research
- Myxozoan Parasites in Aquatic Species
- Plant Stress Responses and Tolerance
- Global Trade and Competitiveness
- Marine Ecology and Invasive Species
- Genomics and Phylogenetic Studies
- Atmospheric and Environmental Gas Dynamics
- Plant tissue culture and regeneration
- Hydrocarbon exploration and reservoir analysis
- Plant pathogens and resistance mechanisms
- Coal and Its By-products
- CRISPR and Genetic Engineering
- Regional Development and Policy
- Wheat and Barley Genetics and Pathology
- Yeasts and Rust Fungi Studies
- Sugarcane Cultivation and Processing
- Economic and Fiscal Studies
- Environmental Science and Water Management
- Genomics, phytochemicals, and oxidative stress
University of York
2016-2024
University of South Bohemia in České Budějovice
2012-2017
Sewanee: The University of the South
2014
Centro di Ricerca per l’Orticoltura
2013
Technology Centre Prague
2011
The genome sequence and genetic diversity of European ash (Fraxinus excelsior) trees reveals the species' varying susceptibility to dieback. Woodlands forests around world are increasingly susceptible spread pests pathogens resulting from climate change global trade. In particular, across Europe North America currently threatened by fungal disease dieback infestation emerald borer beetle, respectively. Against this background, Richard Buggs colleagues report first an tree, Fraxinus...
Durum wheat often faces water scarcity and high temperatures, two events that usually occur simultaneously in the fields. Here we report on stress responsive strategy of durum cultivars, characterized by different use efficiency, subjected to drought, heat a combination both stresses. The cv Ofanto (lower efficiency) activated large set well-known drought-related genes after drought treatment, while Cappelli (higher showed constitutive expression several induced modulation limited number...
Abstract Tree disease epidemics are a global problem, impacting food security, biodiversity and national economies. The potential for conservation breeding in trees is hampered by complex genomes long lifecycles, with most species lacking genomic resources. European Ash tree Fraxinus excelsior being devastated the fungal pathogen Hymenoscyphus fraxineus, which causes ash dieback disease. Taking this system as an example utilizing Associative Transcriptomics first time plant pathology study,...
Summary Polyploidy, the possession of multiple sets chromosomes, has been a predominant factor in evolution and success angiosperms. Although artificially formed allopolyploids show high rate genome rearrangement, genomes cultivars germplasm used for crop breeding were assumed stable structural variation under artificial selection process commercial remained little studied. Here, we show, using repurposed visualization method based on transcriptome sequence data, that rearrangement occurs...
Summary An updated platform was developed to underpin association genetics studies in the polyploid crop species Brassica napus (oilseed rape). Based on 1.92 × 10 12 bases of leaf mRNA seq data, functional genotypes, comprising 355 536 single‐nucleotide polymorphism markers and transcript abundance were scored across a genetic diversity panel 383 accessions using transcriptome reference 116 098 ordered coding DNA sequence ( CDS ) gene models. The use for Associative Transcriptomics first...
Clubroot is a destructive soil-borne pathogen of Brassicaceae that causes significant recurrent reductions in yield cruciferous crops. Although there some resistance oilseed rape (a crop type the species Brassica napus), genetic basis poorly understood. In this study, we used an associative transcriptomics approach to elucidate clubroot pathotype ECD 17/31/31 across diversity panel 245 accessions B. napus. A single nucleotide polymorphism (SNP) association analysis was performed with 256,397...
Significance Plant cell wall (PCW) composition determines the nature and quality of many biologically derived products and, therefore, is a major target for genetic improvement. However, identities functions genes involved in PCW synthesis are still not known. Genome-wide association mapping studies (GWAS) one few ways to identify these genes. collecting precise quantitative phenotype data at scale required GWAS significant challenge. Here, we demonstrate that high-density carbohydrate...
Cold treatment (vernalization) is required for winter crops such as rapeseed (Brassica napus L.). However, excessive exposure to low temperature (LT) in also a stress the semi-winter, early-flowering varieties widely cultivated China. Photosynthetic efficiency one of key determinants, and thus good indicator LT tolerance plants. So far, genetic basis underlying photosynthetic poorly understood rapeseed. Here current study used Associative Transcriptomics identify loci controlling gas...
The genetic diversity of 94 accessions winter oilseed rape (Brassica napus L.), representing past and contemporary material utilized in the Czech breeding programmes, was determined using microsatellites (SSRs), ISSRs AFLPs. All three kinds markers differed range observed polymorphism differentiated clearly each accession. Altogether 89 SSR, 1003 AFLP 53 ISSR were evaluated. Their average rates polymorphic bands 100%, 53.9% 90.6%, respectively, indicating high among accessions. greatest...
Calcium (Ca) and magnesium (Mg) are essential plant nutrients vital for human animal nutrition. Biofortification of crops has previously been suggested to alleviate widespread Ca Mg deficiencies. In this study, new candidate genes influencing the leaf accumulation were identified in young Brassica napus plants using associative transcriptomics ionomics datasets. A total 247 166 SNP markers associated with concentration, respectively, after false discovery rate correction removal SNPs low...
Abstract The development of more productive crops will be key to addressing the challenges that climate change, population growth and diminishing resources pose global food security. Advanced ‘omics techniques can help accelerate breeding by facilitating identification genetic markers for use in marker‐assisted selection. Here, we present validation a new Associative Transcriptomics platform important oilseed crop Brassica juncea . To develop this platform, established pan‐transcriptome...
Rapeseed is a crop of global importance but there need to broaden the genetic diversity available address breeding objectives. Radiation mutagenesis, supported by genomics, has potential supersede genome editing for both gene knockout and copy number increase, detailed knowledge molecular outcomes radiation treatment lacking. To this, we produced re-sequenced panel 1133 M
Abstract Multistep phosphorelay (MSP) signaling integrates hormonal and environmental signals to control both plant development adaptive responses. Type-A RESPONSE REGULATOR (RRA) genes, the downstream members of MSP cascade cytokinin primary response are thought mediate primarily negative feedback regulation (cytokinin-induced) signaling. However, transcriptional data also suggest involvement RRA genes in stress-related By employing evolutionary conservation with well-characterized...
Large nitrogen, phosphorus and potassium fertiliser inputs are used in many crop systems. Identifying genetic loci controlling nutrient accumulation may be useful breeding strategies to increase use efficiency reduce financial environmental costs. Here, variation leaf nitrate concentration across a diversity population of 383 genotypes Brassica napus was characterised. Genetic nitrate, were then identified through Associative Transcriptomics using single nucleotide polymorphism (SNP) markers...
Microspore embryogenesis and cytoplasmic male sterility system (CMS) are two approaches widely exploited in Brassica napus breeding for production of homozygous doubled haploid (DH) lines F1 hybrids respectively. Cytoplasmic is one the most important pollination systems hybrid seed utilisation to quickly prepare fully fertility restorer CMS Ogu-INRA very beneficial. Generally, only a small part microspore-derived embryos used plant regeneration, without any knowledge about their properties....
The selection of desirable genotypes with recessive characteristics, such as self-incompatible plants, is often difficult or even impossible and represents a crucial barrier in accelerating the breeding process. Molecular approaches based on molecular markers can allow breeders to overcome this limitation. use self-incompatibility an alternative hybrid oilseed rape. Unfortunately, stable phenotype-based very time-consuming. development reliable for detecting plants functional genes great...
Non-native species have aroused scientific interest because of their ability to successfully colonise areas which they been introduced, despite sometimes limited genetic variation compared native range. These establish themselves with the aid some pre-existing features favouring them in new environment. Pectinatella magnifica (Leidy, 1851), freshwater magnificent bryozoan, is non-native Europe and Asia. This study was designed determine diversity population structure P. colonies collected...
Until now in Europe has not been cultivated any hybrid cultivar of oilseed rape based on the Shaan 2A cytoplasmic male sterility (CMS), a widely used CMS system China. The aim Czech breeders is to produce new, improved cultivars rapeseed this system. Sterile line (S; rf/rf), its corresponding maintainers (N; rf/rf) and fertility restorers Rf/Rf) were analyzed molecular level for presence functional cytoplasm. Two new primer pairs covering CMS-associated gene (so called orf224-1) present...
In this study, the genetic diversity and relationships among eight millet genera were investigated by molecular morphological data analyses. Sixty-nine accessions analysed using amplified fragment length polymorphism (AFLP) markers, evaluated for traits. Eight AFLP primer pairs successfully 779 bands scored all accessions, with a high level of detected. Nei’s distance varied from 0.0123 to 0.4246 Shannon’s index was estimated at 0.9708. The neighbour joining tree, unweighted...