A5065080624- Nerve injury and regeneration
- Neuroscience and Neuropharmacology Research
- Nitric Oxide and Endothelin Effects
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Mitochondrial Function and Pathology
- S100 Proteins and Annexins
- Neuropeptides and Animal Physiology
- Traumatic Brain Injury and Neurovascular Disturbances
- Nerve Injury and Rehabilitation
- Neuroendocrine regulation and behavior
- Amino Acid Enzymes and Metabolism
- Electrospun Nanofibers in Biomedical Applications
- Spinal Cord Injury Research
- Signaling Pathways in Disease
- Stress Responses and Cortisol
- Diet and metabolism studies
- Cardiac Arrest and Resuscitation
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Neurological Disease Mechanisms and Treatments
- Tryptophan and brain disorders
- Neonatal and fetal brain pathology
- Axon Guidance and Neuronal Signaling
- HER2/EGFR in Cancer Research
Otto-von-Guericke University Magdeburg
2013-2023
University Hospital Magdeburg
2012-2021
Center for Excellence in Molecular Cell Science
2010-2012
Institute of Cell Biology
2010-2012
Institute of Neurobiology
2001-2002
Oregon Health & Science University
2001
Klinik und Poliklinik für Mund-, Kiefer- und Plastische Gesichtschirurgie
1999
Klinikum Magdeburg
1992
University of Göttingen
1991
Reactive oxygen species (ROS) have been implicated as an important causative factor in cell damage, including apoptosis and necrosis. Their proposed actions comprise lipid peroxidation, DNA destruction of the mitochondrial respiratory chain protein modifications. Recent experiments underline importance peroxynitrite, reaction product two potent reactive nitric oxide superoxide. Several fluorogenic compounds used order to determine ROS formation living cells. Besides dihydrorhodamine‐123...
S100B is considered an astrocytic in-situ marker and protein levels in cerebrospinal fluid (CSF) or serum are often used as biomarker for damage dysfunction. However, studies on the human brain rare. Thus, distribution of was studied by immunohistochemistry adult brains to evaluate its cell-type specificity. Contrary glial fibrillary acidic (GFAP), which selectively labels astrocytes shows only faint ependymal immunopositivity, a less uniform staining pattern seen case S100B. Cells with...
Cardiac arrest, and the associated arrest of blood circulation, immediately leads to permanent brain damage because exhaustion oxygen, glucose energy resources in brain. Most hippocampal CA1 neurons die during first week post insult. Molecular data concerning recovery after resuscitation are sparse limited early time period. Expression analysis marker genes via quantitative real-time RT-PCR enables follow up remodeling process. However, proper validation applied normalization strategy is a...
Mesenchymal stem cells (MSCs) were evaluated as an alternative source for tissue engineering of peripheral nerves. MSCs, transdifferentiated or Schwann cultured from male rats grafted into devitalized autologous muscle conduits bridging a 2-cm sciatic nerve gap in female rats. The differentiation potential MSCs and transformed cultivated cell–like was exploited using cocktail cytokines. Polymerase chain reaction the SRY gene confirmed presence implanted grafts. After 6 weeks, regeneration...
In the central nervous system (CNS), neuregulin-1 (NRG-1) proteins function in neuronal migration, differentiation, and survival of oligodendrocytes. The NRG-1 gene codes for at least 15 different isoforms, which may be classified on basis their molecular structure. At two haplotypes associated with schizophrenia. An abnormal expression pattern mRNA was found prefrontal cortex schizophrenic patients comparison to controls. We here show that NRG-1alpha isoform is significantly reduced white...
Accumulating evidence links calcium-overload and oxidative stress to atrial remodeling during fibrillation (AF). Furthermore, appears increase thrombogeneity, characterized by increased expression of adhesion molecules. The aim this study was assess mitochondrial dysfunction stress-activated signal transduction (nuclear factor-kappaB [NF-kappa B], lectin-like oxidized low-density lipoprotein receptor [LOX-1], intercellular molecule-1 [ICAM-1], hemeoxgenase-1 [HO-1]) in tissue AF. Ex vivo...
Purpose: It has been proposed in the literature that Fe 3 O 4 magnetic nanoparticles (MNPs) could be exploited to enhance or accelerate nerve regeneration and provide guidance for regenerating axons. MNPs create mechanical tension stimulates growth elongation of Particles suitable this purpose should possess (1) high saturation magnetization, (2) a negligible cytotoxic profile, (3) capacity magnetize mammalian cells. Unfortunately, materials currently available on market do not satisfy these...
Magnetic nanoparticles (MNPs) offer a large range of applications in life sciences. Applications neurosciences are one focus interest. Unfortunately, not all groups have access to or the possibility develop and produce them for their applications. Hence, they on commercially available particles. Little is known about uptake primary cells. Previously studies mostly reported cellular cell lines. Here we present systematic study magnetic by cells nervous system.We assessed internalization...
Somatostatin mediates its diverse physiological effects through a family of five G-protein-coupled receptors (sst 1 –sst 5 ); however, knowledge about the distribution individual somatostatin receptor proteins in mammalian brain is incomplete. In present study, we have examined regional and subcellular sst 4 rat CNS by raising anti-peptide antisera to C-terminal tail . The specificity affinity-purified antibodies was demonstrated using immunofluorescent staining HEK 293 cells stably...
Because glutathione (GSH) levels in glia play an important role cellular defense against oxidative and nitrosative stress, the present study was designed to GSH primary glial cell cultures. Here we used fluorescence microscopy spectroscopy with monochlorobimane for measurement of intracellular content. Monochlorobimane showed high specificity very little binding protein sulphydryls as ascertained from low intensity fraction cells well GSH-depleted cells. The formation...
In diseases associated with neuronal degeneration, such as Alzheimer's or cerebral ischemia, the cytosolic Ca2+ concentration ([Ca2+]cyt) is pathologically elevated. It still unclear, however, under which conditions induces either apoptotic necrotic cell death. Studying respiration and morphology of rat brain mitochondria, we found that extramitochondrial [Ca2+] above 1 M causes reversible release cytochrome c, a key trigger apoptosis. This event was NO-independent but required influx into...
Nerve allografts are highly antigenic and require the continuous use of immunosuppressive drugs. Neurotoxic complications from immunosuppressant therapy with FK 506 have been noted in central peripheral nervous system although an increased rate axonal regeneration has also noted. Regeneration nerve grafts was assessed a rat model clinically morphometrically after treatment for 2 6 weeks two different doses 506. Good all groups at weeks. A significantly higher axon count observed both...
Abstract 4,5‐diaminofluorescein diacetate (DAF‐2DA) is widely used as a fluorescent probe to detect endogenously produced nitric oxide (NO). Recent reports that refer the high sensitivity of DAF‐2 toward NO prompted us test its efficiency and specificity in mixed murine primary glial culture model, which NO‐synthesizing enzyme inducible synthase (iNOS) expressed by stimulation with lipopolysaccharide (LPS) interferon‐γ (IFN‐γ). Cultures were loaded DAF‐2DA fluorescence was measured using...