A5065744995- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Zebrafish Biomedical Research Applications
- Memory and Neural Mechanisms
- Neurotransmitter Receptor Influence on Behavior
- Tryptophan and brain disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroendocrine regulation and behavior
- Ion channel regulation and function
- X-ray Diffraction in Crystallography
- Cholinesterase and Neurodegenerative Diseases
- Epilepsy research and treatment
- Crystallization and Solubility Studies
- Chromatography in Natural Products
- Berberine and alkaloids research
- Pain Mechanisms and Treatments
- Stress Responses and Cortisol
- Neuropeptides and Animal Physiology
- Neonatal and fetal brain pathology
- Computational Drug Discovery Methods
- Pain Management and Placebo Effect
- Spinal Cord Injury Research
- Diet and metabolism studies
- Nicotinic Acetylcholine Receptors Study
- Bipolar Disorder and Treatment
Medical University of Lublin
2015-2024
University of Oslo
2019-2023
AGH University of Krakow
2021
Abstract Objective To pinpoint the earliest cellular defects underlying seizure onset (epileptogenic period) during perinatal brain development in a new zebrafish model of Dravet syndrome (DS) and to investigate potential disease‐modifying activity 5HT 2 receptor agonist fenfluramine. Methods We used CRISPR/Cas9 mutagenesis introduce missense mutation, designed perturb ion transport function all channel isoforms, into scn1lab , orthologue SCN1A (encoding voltage‐gated sodium alpha subunit...
Central cholinergic dysfunction contributes to acute spatial memory deficits produced by ethanol administration. Donepezil and rivastigmine elevate acetylcholine levels in the synaptic cleft through inhibition of cholinesterases—enzymes involved degradation. The aim our study was reveal whether donepezil (acetylcholinesterase inhibitor) (also butyrylcholinesterase attenuate impairment as induced administration Barnes maze task (primary latency number errors finding escape box) rats....
Zingiber officinale is one of the most frequently used medicinal herbs in Asia. Using rodent seizure models, it was previously shown that hydroethanolic extract exerts antiseizure activity, but active constituents responsible for this effect have not been determined. In paper, we demonstrated methanolic anticonvulsant activity pentylenetetrazole (PTZ)-induced hyperlocomotion assay larval zebrafish. Next, isolated 6-gingerol (6-GIN)—a major constituent rhizoma. We observed 6-GIN exerted...
Gastric cancer (GC) has high incidence rates and constitutes a common cause of mortality. Despite advances in treatment, GC remains challenge therapy which is why novel treatment strategies are needed. The interest natural compounds increased significantly recent years because their numerous biological activities, including anti-cancer action. isolation the bioactive from Coptis chinensis Franch was carried out with Centrifugal Partition Chromatography (CPC) technique, using biphasic solvent...
Pterostilbene (PTE), a natural dimethylated analog of resveratrol, possesses numerous health-beneficial properties. The ability PTE to cross the blood-brain barrier raised possibility that this compound may modulate central nervous system functions, including seizure activity. aim our study was investigate activity in larval zebrafish pentylenetetrazole (PTZ) assay and three acute tests mice, i.e., maximal electroshock threshold (MEST), 6 Hz-induced psychomotor intravenous (iv) PTZ tests....
Abstract The CACNA1A gene encodes the pore-forming α1 subunit of voltage-gated P/Q type Ca 2+ channels (Ca v 2.1). Mutations in this gene, among others, have been described patients and rodents suffering from absence seizures episodic ataxia 2 with/without concomitant seizures. In study, we aimed for first time to assess phenotypic behavioral alterations larval zebrafish with partial cacna1aa knockdown, placing special emphasis on changes epileptiform-like electrographic discharges brains....
Palmatine (PALM) and berberine (BERB) are widely identified isoquinoline alkaloids among the representatives of Berberidaceae botanical family. The antiseizure activity BERB was shown previously in experimental epilepsy models. We assessed effect PALM a pentylenetetrazole (PTZ)-induced seizure assay zebrafish, with as an active reference compound. Both were isolated from methanolic root extract Berberis sibirica by counter-current chromatography, their ability to cross blood–brain barrier...
Many people around the world suffer from neurodegenerative diseases associated with cognitive impairment. As life expectancy increases, this number is steadily rising. Therefore, it extremely important to search for new treatment strategies and discover substances potential neuroprotective and/or cognition-enhancing effects. This study focuses on investigating of astragaloside IV (AIV), a triterpenoid saponin proven acetylcholinesterase (AChE)-inhibiting activity naturally occurring in root...
The global increase in resorting to artificial nutritional formulas replacing breastfeeding has been identified among the complex causes of obesity epidemic infants and children. One factors recently recognized influence metabolism weight gain is kynurenic acid (KYNA), an agonist G protein-coupled receptor (GPR35). Therefore aim study was determine concentration KYNA comparison with its level human breast milk evaluate developmental changes rats exposed enriched diet during time...
The main aim of the study was to assess acetylcholinesterase-inhibitory potential triterpenoid saponins (astragalosides) found in roots Astragalus mongholicus. For this purpose, TLC bioautography method applied and then IC50 values were calculated for astragalosides II, III IV (5.9 μM; 4.2 μM, 4.0 respectively). Moreover, molecular dynamics simulations carried outto affinity tested compounds POPC POPG-containing lipid bilayers, which case are models blood-brain barrier (BBB). All determined...
Postnatal depression is a common and severe complication of childbirth. It an important public health problem with significant implications for both mothers children. The exact mechanisms underlying the factors influencing occurrence postnatal remain unclear. literature suggests that certain dietary deficiencies during pregnancy period may contribute to greater risk maternal depression. This review focuses on role selenium in collects evidence from published interventional observational...
(R)-7 [(R)-AS-1] showed broad-spectrum antiseizure activity across in vivo mouse seizure models: maximal electroshock (MES), 6 Hz (32/44 mA), acute pentylenetetrazol (PTZ), and PTZ-kindling. A remarkable separation between CNS-related adverse effects was also observed. In vitro studies with primary glia cultures COS-7 cells expressing the glutamate transporter EAAT2 enhancement of uptake, revealing a stereoselective positive allosteric modulator (PAM) effect, further supported by molecular...
The pharmacological activation of the GPR39 receptor has been proposed as a novel strategy for treating seizures; however, this hypothesis not verified experimentally. TC-G 1008 is small molecule agonist increasingly used to study function but validated using gene knockout. Our aim was assess whether produces anti-seizure/anti-epileptogenic effects in vivo and are mediated by GPR39. To obtain goal we utilized various animal models seizures/epileptogenesis knockout mice model. Generally,...
In our recent studies, we identified compound N-benzyl-2-(2,5-dioxopyrrolidin-1-yl)propanamide (AS-1) as a broad-spectrum hybrid anticonvulsant which showed potent protection across the most important animal acute seizure models such maximal electroshock (MES) test, subcutaneous pentylenetetrazole (s.c. PTZ) and 6-Hz (32 mA) test in mice. Therefore, AS-1 may be recognized candidate for new effective different types of human epilepsy with favorable safety margin profile determined rotarod aim...
Preclinical models of seizures and epilepsy in rodents contributed substantially to the discovery currently available antiseizure medications. These were also broadly used for investigation processes epileptogenesis. Nevertheless, rodent pose some limitations, thus, new using alternative species are high demand. The aim this study was describe a model seizures/epilepsy induced by cholinomimetic agent, pilocarpine (PILO), larval zebrafish.
Glutamate is essential for learning and memory processes, acute chronic exposures to ethanol (or protracted abstinence) alter glutamatergic transmission. In the current study, we investigated effects of VU-29, positive allosteric modulator metabotropic glutamate 5 (mGlu5) receptor, on ethanol- withdrawal-induced impairment novel object recognition (NOR) task in rats. The influence VU-29 (30 mg/kg) retrieval was measured (a) at 4-h delay after administration, as well (b) withdrawal (24 48 h)...