A5029006331- Microbial Community Ecology and Physiology
- Bacterial biofilms and quorum sensing
- Polar Research and Ecology
- Plant-Microbe Interactions and Immunity
- Antimicrobial agents and applications
- Antimicrobial Peptides and Activities
- Collagen: Extraction and Characterization
- Antimicrobial Resistance in Staphylococcus
- Bone Tissue Engineering Materials
- Legume Nitrogen Fixing Symbiosis
- Wound Healing and Treatments
- Oral microbiology and periodontitis research
- Antibiotic Resistance in Bacteria
- Protist diversity and phylogeny
- Biochemical and Structural Characterization
- Antibiotic Use and Resistance
- Pharmaceutical and Antibiotic Environmental Impacts
- Silk-based biomaterials and applications
- Plant tissue culture and regeneration
- Mycorrhizal Fungi and Plant Interactions
- Biocrusts and Microbial Ecology
- Marine Biology and Environmental Chemistry
- Legionella and Acanthamoeba research
- Laser Applications in Dentistry and Medicine
- Diverse Scientific Research in Ukraine
Institute of Molecular Biology and Genetics
2020-2025
National Academy of Sciences of Ukraine
2020-2025
Kyiv National University of Technologies and Design
2020-2025
Vytautas Magnus University
2024
Danylo Zabolotny Institute of Microbiology and Virology
2018-2019
Several model plants are known to respond bacterial quorum sensing molecules with altered root growth and gene expression patterns induced resistance plant pathogens. These compounds may represent novel elicitors that could be applied as seed primers enhance cereal crop pathogens abiotic stress improve yields. We investigated whether the acyl-homoserine lactone N-hexanoyl-L-homoserine (C6-HSL) impacted winter wheat (Triticum aestivum L.) germination, development productivity, using two...
The choice of effective biocides used for routine hospital practice should consider the role disinfectants in maintenance and development local resistome how they might affect antibiotic resistance gene transfer within microbial population. Currently, there is little understanding different contribute to eDNA release that may subsequent environmental retention. Here, we investigated from mature biofilms two opportunistic model strains Pseudomonas aeruginosa ATCC 27853 (PA) Staphylococcus...
Introduction Periodontal diseases are known to be associated with polymicrobial biofilms and inflammasome activation. A deeper understanding of the subgingival cytological (micro) landscape, role extracellular DNA (eDNA) during periodontitis, contribution host immune eDNA persistence, may improve our mechanisms underlaying severe forms periodontitis. Methods In this work, biolfilms developing on biologically neutral polyethylene terephthalate films placed in gingival cavities patients...
Collagen and its derivates are typically obtained by extracting them from fresh animal tissues. Lately, however, there has been an increased interest in obtaining collagen other sources, such as waste material, because of the growing trend to replace synthetic materials with sustainable, natural counterparts various industries, well ensure a rational revalorization. In this paper, was non-tanned leather production, taken at different stages production process: limed pelt, delimed fleshings....
Pseudomonas fluorescens SBW25 is a model soil- and plant-associated bacterium capable of forming variety air–liquid interface biofilms in experimental microcosms on plant surfaces. Previous investigations have shown that cellulose the primary structural matrix component robust well-attached Wrinkly Spreader biofilm, as well fragile Viscous Mass biofilm. Here, we demonstrate both include extracellular DNA (eDNA) which can be visualized using confocal laser scanning microscopy (CLSM),...
Novel antibiotic combinations may act synergistically to inhibit the growth of multidrug-resistant bacterial pathogens but predicting which combination will be successful is difficult, and standard antimicrobial susceptibility testing not identify important physiological differences between planktonic free-swimming biofilm-protected surface-attached sessile cells. Using a nominally macrolide-resistant model Klebsiella pneumoniae strain (ATCC 10031) we demonstrate effectiveness several...
A therapeutic combination of azithromycin (AZM) and colistin methanesulfonate (CMS) was shown to be effective against both non-PDR PDR Klebsiella pneumoniae biofilms in vitro. These anti-biofilm effects, however, may not correlate with effects observed standard plate assays, nor will they representative vivo action. After all, biofilm-associated infection processes are also impacted by the presence wound bed components, such as host cells or fluids, which can all affect antibiotic...
Antimicrobial resistance recognised as a major global health problem and it poses significant challenge in conflict zones, such the Russia-Ukraine war. This case study focuses on 32-year-old soldier who sustained combat-related injuries, including extensive wound infections caused by multidrug-resistant pan-resistant bacteria was successfully treated with azithromycin-meropenem combination therapy. The emergence of bacteria, particularly pandrug-resistant strain Pseudomonas aeruginosa,...
Staphylococcus aureus (S. aureus) is a causative agent of many hospital- and community-acquired infections with the tendency to develop resistance all known antibiotics. Therefore, development novel antistaphylococcal agents urgent need. Sortase A considered promising molecular target for agents. The main aim this study was identify sortase inhibitors. In order find agents, we performed phenotypic screening library containing 15512 compounds against S. ATCC43300. docking hits using DOCK...
Biofilm formation, or microfouling, is a basic strategy of bacteria to colonise surface and may happen on surfaces any nature whenever are present. Biofilms hard eradicate due the matrix in which reside, consisting strong, adhesive adaptive self-produced polymers such as eDNA functional amyloids. Targeting biofilm be promising prevent formation. Here, femtosecond laser irradiation was used modify stainless steel order introduce either conical spike groove textures. The resulting topography...
Deschampsia antarctica and Colobantus quitensis are the only two vascular plants colonized on Antarctic continent, which is usually exposed to extreme environments. Endophytic bacteria residing within plant tissues can exhibit diverse adaptations that contribute their ecological success potential benefits for hosts. This study aimed characterize 12 endophytic bacterial strains isolated from these plants, focusing functional roles like growth promotion, antifungal activities, tolerance salt...
Several model plants are known to respond bacterial quorum sensing molecules with altered root growth and gene expression patterns induced resistance plant pathogens. These compounds may represent novel elicitors that could be applied as seed primers enhance cereal crop pathogens abiotic stress improve yields. We investigated whether the acyl-homoserine lactone N-hexanoyl-L-homoserine (C6-HSL) impacted winter wheat (Triticum aestivum L.) germination, development productivity, using two...
Metal-based nanoparticles (MNPs) are promoted as effective compounds in the treatment of bacterial infections and possible alternatives to antibiotics. These MNPs known affect a broad spectrum microorganisms using multitude strategies, including induction reactive oxygen species interaction with inner structures cells. The aim this review was summarise latest studies about effect metal-based on pathogenic biofilm formed wounds, examples Gram-positive bacterium Staphylococcus aureus...
This study presents high-quality draft genome assemblies of six bacterial strains isolated from the roots wheat grown in soil contaminated with cadmium. The results this will help to elucidate at molecular level how heavy metals affect interactions between beneficial rhizobacteria and crop plants.
The establishment of O2 gradients in liquid columns by bacterial metabolic activity produces a spatially-structured environment. This high-O2 region at the top that represents an un-occupied niche which could be colonised biofilm-competent strains. We have used this to develop experimental model system using soil-wash inocula and serial-transfer approach investigate changes community-based biofilm-formation productivity. involved 10 transfers mixed-community or biofilm-only samples over...
The choice of effective biocides used for routine hospital practice should consider the role disinfectants in maintenance and development local resistome how they might affect antibiotic resistance gene transfer within microbial population. Currently, there is little understanding different contribute to eDNA release that may subsequent environmental retention. Here we investigated affected from mature biofilms two opportunistic model strains Pseudomonas aeruginosa ATCC 27853 (PA)...
Keywords: fluorescence microscopy, styryl dyes, S. aureus, functional amyloids, bacterial biofilm, laser scanning confocal microscopy